Mouse anti human mouse rat gapdh g3pdh monoclonal antibody

NB1RGB cells were seeded into 6-well plates at a density of 100,000 cells/well and infected next day with the indicated retroviral supernatants (OSKM) for 24 h. Cells were treated with 100 μg/mL CHX on day 5 after infection for varying lengths of time (0h, 8h, 24h, 48h, 56h, and 72h). Culture medium was changed next day after CHX treatment. Total proteins were collected and subjected to Western blot for KLF4, GAPDH, Ubiquitin. Briefly, the cells were harvested at indicated time point with 0.2 mL of Laemmli sample buffer and boiled at 95°C for 5 min. Automatic capillary Western Blot analysis was performed with Wes device (Protein Simple) with Anti-Mouse Detection Module kit (Protein Simple). Antibodies used: Anti-KLF4 polyclonal Rabbit Ig Antibodies at 1:50 dilution (MBL), Mouse Anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody 1:2000 dilution (R&D), Ubiquitin (P4D1) Mouse mAb 1:50 dilution (Cell Signaling Technology).

NB1RGB cells were seeded into 6-well plates at a density of 100,000 cells/well and infected the next day with the indicated retroviral supernatants (OSKM) for 24 h. On day 5 after infection, total proteins were collected and subjected to Western blot for KLF4 and GAPDH. Automatic capillary Western Blot analysis was performed using Wes device (Protein Simple) with Anti-Mouse Detection Module kit (Protein Simple). Antibodies used: anti-FLAG monoclonal mouse antibody (Sigma), mouse anti-Human/Mouse/Rat GAPDH/G3PDH Monoclonal Antibody (R&D).