Masson s trichrome stain kit

Fresh liver tissues were fixed overnight in 10% neutral buffered formalin. Hematoxylin and eosin staining was performed using a commonly used protocol. Collagen staining was performed using Masson’s trichrome stain kit from Thermo Fisher Scientific (cat #87019). Histology was assessed by a pathologist with representative images captured by the Olympus VS120 slide scanning system shown in the figures.

To label the newly formed bone, two doses of calcein (25 mg/kg, catalog no. C0875, Sigma-Aldrich) were injected intraperitoneally (i.p.) to 8-month-old male WT and 5xFAD mice eight days apart. Seventy-two hours after the second calcein injection, mice were euthanized for tissue harvest. Tissue processing and histomorphometry on the distal portion of the femur were performed at the MGH Center for Skeletal Research Histology & Histomorphometry Core according to the American Society for Bone and Mineral Research guidelines (Dempster et al., 2013 (link)). The Masson's Trichrome stain kit from Thermo Fisher (catalog no. 87019) was used to visualize osteoblasts, and the Leukocyte Acid Phosphatase (TRAP) Kit from Sigma-Aldrich (catalog no. 387A) was used to identify osteoclasts, with Fast Green (catalog no. F7252, Sigma-Aldrich) as counterstaining.