All experiments were approved by and performed in agreement with the guidelines of the Institutional Animal Care and Use Committee (IACUC) at University of Colorado School of Medicine. 10 week-old mice used in experiments were: DAT-IRES-Cre heterozygote mice (Slc6a3IRES-Cre, Jackson Laboratory stock #006660), ChAT-IRES-Cre heterozygote mice (Chattm2(cre)Lowl/J, Jackson Laboratory stock #006410), VGLUT2 conditional knockdown (Slc17a6+/fl ; Slc6a3+/IRESCre Jackson Laboratory stock #006660 x #007583), CaMKII-Cre heterozygote mice (generated from WT and homozygous B6.Cg-Tg(Camk2a-cre)T29-1Stl/J, Jackson Laboratory stock# 005359), and wild-type C57BL/6J mice (Jackson Laboratory stock #000664). Both male and female mice were used for all experiments.
Slc6a3irescre
Manufactured by Jackson ImmunoResearch
Slc6a3IRESCre, is a genetic construct that contains the Slc6a3 gene, which encodes the dopamine transporter, and an IRES-Cre sequence. The IRES-Cre element allows for the expression of Cre recombinase under the control of the Slc6a3 promoter. This tool can be used for genetic manipulation and studies related to the dopamine system.
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Lab products found in correlation
3 protocols using slc6a3irescre
1
Mouse Genetics for Neuroscience Research
2
Cre-expressing Mouse Model for Neuroscience
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Male mice (8–12 weeks old) expressing Cre recombinase under the control of the DA transporter (DATcre) (Slc6a3IRESCre, Jackson stock 006660, The Jackson Laboratory) were bred on a C57BL/6J genetic background. Mice and adult (10 months) male and female Lewis rats (Envigo) were maintained under standard temperature conditions with a 12:12‐h light:dark cycle; conventional diet and water were available ad libitum. All experiments were approved by the University of Pittsburgh Institutional Animal Care and Use Committee (for rats), and the University of California, San Diego (UCSD) Institutional Animal Care and Use Committee (for mice), which are AAALAC‐accredited. Animals were cared for in accordance with all appropriate animal care guidelines according to the ARRIVE guidelines for reporting animal research. All efforts were made to ameliorate animal suffering. Rodent immunohistochemistry procedures can be found in Appendix S1 .
3
Genetic Manipulation of Dopamine and VGLUT2 Neurons
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Mice were used in accordance with protocols approved by the University of California, San
Diego Institutional Animal Care and Use Committee (protocol no. S12080). Mice expressing
Cre under the control of DAT (Slc6a3IRESCre,
Jackson stock no. 006660) or VGLUT2
(Slc17a6IRESCre, Jackson stock no. 016963)
regulatory elements were obtained from The Jackson Laboratory and then bred in house. Mice
lacking VGLUT2 in dopamine neurons (VGLUT2 cKOs)
(Slc17a6flox/flox;
Slc6a3+/IRESCre) were generated as
described previously.23 (link) Mice were (>10 generations) back-crossed to
C57BL/6 background. Both sexes were used for experiments. Mice were group-housed on a 12 h
light–dark cycle and with food and water available ad libitum.
Diego Institutional Animal Care and Use Committee (protocol no. S12080). Mice expressing
Cre under the control of DAT (Slc6a3IRESCre,
Jackson stock no. 006660) or VGLUT2
(Slc17a6IRESCre, Jackson stock no. 016963)
regulatory elements were obtained from The Jackson Laboratory and then bred in house. Mice
lacking VGLUT2 in dopamine neurons (VGLUT2 cKOs)
(Slc17a6flox/flox;
Slc6a3+/IRESCre) were generated as
described previously.23 (link) Mice were (>10 generations) back-crossed to
C57BL/6 background. Both sexes were used for experiments. Mice were group-housed on a 12 h
light–dark cycle and with food and water available ad libitum.
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