B95-8-ZHT cells which are derived from B95-8 marmoset cells and stably express EBV BZLF1 in a 4-hydroxytamoxifen-dependent manner21 (link), were cultured in RPMI1640 (Nacalai Tesque, Kyoto, Japan) supplemented with 10% fetal bovine serum (FBS; Merck, Darmstadt, Germany), streptomycin/penicillin, and 2-mercaptoethanol (Nacalai Tesque). LCLs were cultured in RPMI1640 (Nacalai Tesque) supplemented with 20% FBS (Merck), streptomycin/penicillin, 2-mercaptoethanol (Nacalai Tesque), K3 (Ajinomoto Bio-Pharma Services, Osaka, Japan), Ciclosporin (Novartis Pharma, Basel, Switzerland), IL-6, and BAFF (R & D systems, Minneapolis, MN, USA) (LCL medium).
Ciclosporin
Manufactured by Novartis
Sourced in Japan, Switzerland
Ciclosporin is a laboratory product manufactured by Novartis. It is a cyclic peptide compound used for research and scientific applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ciclosporin
1
EBV BZLF1 Inducible B95-8 Cell Culture
2
Cerebellum Nanoparticle Transplantation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Twenty-four hours before transplantation, mice were injected intraperitoneally with 10 mg/kg ciclos-porin (Novartis, Basel, Switzerland). Stereotaxic injections of GNPs into the cerebellum were carried out as previously described.5 (link) Following injection, Alzet pumps (DURECT, Cupertino, CA, USA) filled with ciclosporin (10 mg/kg per day) were transplanted subcutaneously and replaced monthly. Procedures for sample preparation and immunolabeling were performed as described.5 (link) All antibodies and concentrations used are listed in Table2 . For BrdU incorporation, mice received BrdU intraperitoneally (50 mg/kg) every alternate day for 30 days, starting at 5 months, and sacrificed at 7 months. After antigen retrieval, sections were treated with 2 mol/L HCl for 30 min followed by 0.1 mol/L Sodium Borate for 5 min, and normal labeling performed.
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