Sections from frozen tissues were cut by cryostat (Leica Microsystems, Wetzlar, Germany). For detection of Laminin, the frozen section was immunostained by using anti-laminin antibody (ab80, Abcam, Cambridge, UK; 1:100) and Alexa Fluor 488-conjugated second antibody (Life Technologies; 1:400) with Can Get Signal Immunostain according to manufacturer’s protocol (Toyobo, Osaka, Osaka). Images were acquired with microscopes (BZ-9000, Keyence, Osaka, Osaka) equipped with imaging software (BZ-II, Keyence). Cross-sectional area was calculated by using ImageJ software (version: 1.53e).
Alexa fluor 488 conjugated second antibody
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom, United States
The Alexa Fluor 488-conjugated second antibody is a fluorescent-labeled secondary antibody used in various immunodetection techniques. It is designed to specifically bind and detect primary antibodies, enabling visualization and signal amplification in applications such as immunohistochemistry, flow cytometry, and Western blotting.
Automatically generated - may contain errors
2 protocols using alexa fluor 488 conjugated second antibody
1
Laminin Immunostaining in Frozen Tissue
2
Immunostaining Slow-Twitch Muscle Fibers
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For detection of slow-twitch muscle fibers, muscle sections were immunostained by using anti-slow skeletal myosin heavy chain antibody (1:200, NOQ7.5.4D; Sigma-Aldrich, St. Louis, MO, USA) and Alexa Fluor 488-conjugated second antibody (1:400; Life Technologies, Carlsbad, CA, USA) with Can Get Signal Immunostain according to manufacturer's protocol (Toyobo, Osaka, Osaka). Images were acquired using a fluorescent stereo microscope (Leica, Germany).
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