The chromatographic analysis of ibuprofen and diclofenac in the muscle tissue and water samples was performed using Ultra-High-Performance Liquid Chromatography coupled with triple Quadrupole Mass Spectrometry Acquity H-Class from Waters (Milford, Massachusets, USA). The operation of the equipment and the quanti cation of the data was performed with MassLynx 4.1 software. Simultaneous determination of the two drugs was performed with a UHPLC KINETEX Core-Shell C18 column (1.7µm C18 100Å, 50 x 2.1mm) from Phenomenex (Torrance, California, United States). To perform the separation, reversed-phase gradient elution was used as shown in Table 2, with a total execution time of 7.0 min. The mobile phase consisted of 0.05% acetic acid (A) and acetonitrile: MeOH (50:50 v/v) (B), with an injection ow of 0.3mL/min and injection volume of 20 μL.
The column was maintained at a temperature of 40 ° C and the sample at 15 ° C. The capillary voltage of Waters Xevo TQD was set at 3.5 kV for ESI -. The source temperature was set at 130 ° C and the desolvation gas temperature at 350 ° C, with a ow of 1000 L/h and a cone gas ow of 100 L/h. To obtain greater sensitivity of the two drugs, conditions such as precursor ion, retention times, cone voltage, collision energy, and ion ratios were optimized, and are described in detail in Table 3.
The column was maintained at a temperature of 40 ° C and the sample at 15 ° C. The capillary voltage of Waters Xevo TQD was set at 3.5 kV for ESI -. The source temperature was set at 130 ° C and the desolvation gas temperature at 350 ° C, with a ow of 1000 L/h and a cone gas ow of 100 L/h. To obtain greater sensitivity of the two drugs, conditions such as precursor ion, retention times, cone voltage, collision energy, and ion ratios were optimized, and are described in detail in Table 3.