Reichert microscope

Manufactured by Reichert Technologies

Sourced in United States

The Reichert microscope is a precision optical instrument designed for visual examination and analysis of microscopic specimens. It utilizes advanced optics to provide high-magnification, clear, and detailed images of samples. The microscope's core function is to enable detailed observation and inspection of small-scale objects and materials.

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Lab products found in correlation

Axiocam erc5, by Zeiss (2 mentions) Stemi 2000 c, by Zeiss (2 mentions) Mira3 xmu, by TESCAN (1 mentions) Stemi 508, by Zeiss (1 mentions) Mira 3 xmu scanning electron microscope, by TESCAN (1 mentions) Dichloran rose bengal chloramphenicol agar, by BD (1 mentions) Tween 80, by Tokyo Chemical Industry (1 mentions) Neubauer s improved counting chamber hemocytometer, by Marienfeld (1 mentions)

3 protocols using reichert microscope

Preparation and Imaging of Cross-Sections

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The CitoPress-10 hot mounting press (Struers, DK) was used for the PolyFast and LevoFast resins.
A Leica EM ACE 600 high-vacuum coater was used for the application of a carbon coating of 30 nm in Preparation B.
The grinding and polishing of the cross-sections were done as described in Jaques and Zikmundová (unpublished). Before the sample was reached, the polishing mode was changed from wet to dry^{47 (link)}.
The first observations of the cross-sections were made under the Stemi 2000-C and Stemi 508 stereomicroscopes (Zeiss, DE) and a Reichert microscope (Reichert Technologies, US) coupled to an Axiocam ERc 5 s (Zeiss, DE).
The scanning electron microscope used for this study is a MIRA3 XMU (Tescan, CZ). Both the high-vacuum (5 mbar to 9 × 10–5 mbar) mode and the low-vacuum (0.07 mbar to 5 mbar) mode with a secondary electron detector (LVSTD mode) were used.

Jaques V.A., Zikmundová E., Holas J., Zikmund T., Kaiser J, & Holcová K. (2022). Conductive cross-section preparation of non-conductive painting micro-samples for SEM analysis. Scientific Reports, 12, 19650.

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Microscopic Imaging of Micro-Samples

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The visual description of the micro-sample and cross-section surface was made under the Stemi 2000-C and the Stemi 508 stereo microscopes (both Zeiss, DE) and a Reichert microscope (Reichert Technologies, US), both coupled to an Axiocam ERc 5 s (Zeiss, DE).
The high-resolution imaging was performed in the StAN laboratory of CEITEC-BUT using the MIRA3 XMU scanning electron microscope (Tescan, CZ; high-vacuum 5 mbar to 9 × 10⁻⁵ mbar). A low-vacuum (0.07–5 mbar) secondary electron detector (LVSTD; Tescan, CZ) was used.

Jaques V.A., Zikmundová E., Holas J., Zikmund T., Kaiser J, & Holcová K. (2022). Cyclododecane shaping, sublimation rate and residue analysis for the extraction of painting micro-samples from resin cross-sections. Scientific Reports, 12, 19654.

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Fungal Spore Preservation and Quantification

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Frozen isolates of each mold strain were allowed to thaw on ice and spotted onto the surface of malt extract agar plates (Difco, Franklin Lakes, NJ), followed by incubation at 25°C for 30 d until spore formation. Plates were flooded with PBS containing 0.1% Tween 80 (Tokyo Chemical Industry Co. Ltd., Tokyo, Japan), and then gently scraped using a sterile cell spreader to release spores and mycelia. Mycelia were filtered from the mold suspensions by passing through 4 layers of sterile cheesecloth. Spore formation was confirmed microscopically (Reichert microscope, Reichert Technologies, Depew, NY). In sterile 50-mL conical tubes (VWR, Radnor, PA), spore suspensions were supplemented with glycerol at 50% (vol/vol). Spore suspensions were stored at -80 ± 2°C until use.
For each mold strain, spore suspension concentrations were determined by microscopy with a Neubauer's improved counting chamber hemocytometer (Paul Marienfeld GmbH & Co. KG, Lauda-Königshofen, Germany). Spore counts were confirmed by plating in duplicate on dichloran rose bengal chloramphenicol agar (Becton, Dickinson and Co., Sparks, MD), followed by incubation at 25°C for 5 d.

Makki G.M., Kozak S.M., Jencarelli K.G, & Alcaine S.D. (2021). Evaluation of the efficacy of commercial protective cultures to inhibit mold and yeast in cottage cheese. Journal of dairy science, 104(3).

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