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Sorafenib free base

Manufactured by LC Laboratories
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Sorafenib (free base) is a chemical compound supplied by LC Laboratories. It is a small-molecule tyrosine kinase inhibitor. The core function of this product is as a research tool for scientific investigations.

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Lab products found in correlation

Fluorescein sodium salt, by Merck Group (1 mentions) N hexane, by Merck Group (1 mentions) Ethyl vinyl ether, by Merck Group (1 mentions) Palladium on carbon, by Merck Group (1 mentions) M chloroperbenzoic acid, by Merck Group (1 mentions) Cyanine5 amine, by Lumiprobe (1 mentions) Triton x 100, by Merck Group (1 mentions) Pluronic f 127, by Merck Group (1 mentions) Polyvinyl alcohol (pva), by Merck Group (1 mentions) Human serum albumin (hsa), by Merck Group (1 mentions) Acetone, by Merck Group (1 mentions) Dichloromethane, by Merck Group (1 mentions) Mannitol, by Merck Group (1 mentions) Sodium azide, by Merck Group (1 mentions) Polysorbate 80, by Merck Group (1 mentions) Magnevist, by Bayer (1 mentions) D trehalose dehydrate, by Merck Group (1 mentions) Resomer rgp d 5055, by Evonik (1 mentions) Resomer rg 502h, by Evonik (1 mentions) Adp glo kinase assay, by Promega (1 mentions)

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Sorafenib (90 citations)

3 protocols using sorafenib free base

1

Polymer-Based Emulsifier Synthesis

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Poly(vinyl alcohol)-(PVA, Mw=30,000-70,000, 87-90% hydrolysed), polyoxyethylene sorbitan monooleate-(Tween 80) and Pluronic F68 emulsifiers; ethanol, acetone, dimethyl sulfoxide (DMSO), sodium azide, 1-ethyl-3(3dimethylaminopropyl) carbodiimide (EDC), Nhydroxysuccinimide (NHS) and 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT), abs. toluene, tripropylamine, p-toluenesulfonyl hydrazide, xylenes, BHT, 1,4dioxane, TFA, abs. THF, LiAlH 4 , abs. diethyl ether, Nmethylmorpholine N-oxide, OsO 4 , n-hexane, 1,5cyclooctadiene, m-chloroperbenzoic acid, 1,6-heptadiene-4-ol, palladium on carbon, ethyl vinyl ether and fluorescein sodium salt were obtained from Sigma Aldrich, ruthenium catalyst G2 was from Materia Inc. Sorafenib (free base) was purchased from LC Laboratories (US). Cyanine 5 amine was the product of Lumiprobe Gmbh (Germany).
Feczkó T., Merza G., Babos G., Varga B., Gyetvai E., Trif L., Kovács E, & Tuba R. (2019). Preparation of cubic-shaped sorafenib-loaded nanocomposite using well-defined poly(vinyl alcohol alt-propenylene) copolymer. International journal of pharmaceutics, 562.
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2

Polymeric Nanoparticle Formulation Development

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Resomer® RG 502H (PLGA, lactide: glycolide: 50:50, inherent viscosity: 0.16–0.24 dL/g), Resomer® RG 752H (PLGA, lactide:glycolide: 75:25 inherent viscosity 0.14–0.22 dL/g), and block copolymer Resomer® RGP d5055 (PEG-PLGA, PEG content: 3–7% (m/m), inherent viscosity: 0.93 dL/g) were obtained from Evonik Industries AG (Essen, Germany). PVA (Mw = 30,000–70,000, 87–90% hydrolysed), polysorbate 80, Triton X-100, Pluronic F127, poly(methacrylic acid sodium salt) emulsifiers, dichloromethane (DCM), acetone, dimethyl sulfoxide (DMSO), sodium azide, D-trehalose dehydrate, mannitol, polyethyleneimine (PEI) (MW 25 kDa), 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC), N-hydroxysuccinimide (NHS), Gd-DTPA, and HSA were obtained from Sigma Aldrich (St. Louis, MO, USA). Sorafenib (free base) was purchased from LC Laboratories (Woburn, MA, USA). Magnevist® was purchased from Bayer AG (Leverkusen, Germany). The micro bicinchoninic acid (µBCA) protein assay kit was bought from Pierce Biotechnology, Inc. (Waltham, MA, USA).
Feczkó T., Piiper A., Pleli T., Schmithals C., Denk D., Hehlgans S., Rödel F., Vogl T.J, & Wacker M.G. (2019). Theranostic Sorafenib-Loaded Polymeric Nanocarriers Manufactured by Enhanced Gadolinium Conjugation Techniques. Pharmaceutics, 11(10), 489.
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3

Synthesis and Kinase Inhibition Assays

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The detailed synthetic procedures and methods of t-CUPM were described in the supplementary data. t-AUCB was also synthesized with our previously published method [14 (link)]. Sorafenib (free base) was purchased from LC Laboratories (Worburn, MA).
sEH enzyme assay followed our previous published method[14 (link)]. Recombinant kinase activity assay of b-Raf and c-Raf was performed using the ADP-Glo™ Kinase Assay (Promega, Madison, WI) as described by the manufacturer. IC50 values were calculated by quantifying the end-point ADP production from each kinase reaction. Individual data sets were performed in duplicate and each IC50 was determined by three separate experiments. The data were fit to a saturation curve using KaleidaGraph graphing program (Synergy Software) to determine the inhibition at 50% activity (IC50).
Liao J., Hwang S.H., Li H., Yang Y., Yang J., Wecksler A.T., Liu J.Y., Hammock B.D, & Yang G.Y. (2015). Inhibition of mutant KrasG12D–initiated murine pancreatic carcinoma growth by a dual c-Raf and soluble epoxide hydrolase inhibitor t-CUPM. Cancer letters, 371(2), 187-193.
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