Transcription assay system

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Transcription Assay System is a laboratory instrument designed for the quantitative analysis of gene expression and transcriptional activity. It provides a reliable and accurate method for measuring the level of RNA transcripts in a given sample.

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Lab products found in correlation

Fluostar optima plate reader, by BMG Labtech (2 mentions) Rocaglamide, by Enzo Life Sciences (1 mentions) Fluostar optima, by BMG Labtech (1 mentions)

Spelling variants of this product

EZ-Detect Transcription Factor Assay System ELISA kit (2 citations)

3 protocols using transcription assay system

Evaluation of NF-κB Binding Affinity

Cited 1 time

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The NF-κB assay was performed according to established protocol (12 (link)). Tested samples were dissolved in dimethyl sulfoxide (DMSO). Nuclear extract from HeLa cells was evaluated using the Transcription Assay System (Pierce Biotechnology, Rockford, IL, USA). The assay was used to evaluate the binding affinity to biotinylated consensus sequence of the NF-κB subunit p65. Luminescence was detected using Fluostar Optima plate reader (BMG Labtech Inc,). Rocaglamide was used as a positive control.

Acuña U.M., Mo S., Zi J., Orjala J, & Carcache de Blanco E.J. (2018). Hapalindole H Induces Apoptosis as an Inhibitor of NF-κB and Affects the Intrinsic Mitochondrial Pathway in PC-3 Androgen-insensitive Prostate Cancer Cells. Anticancer research, 38(6), 3299-3307.

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Quantifying NF-κB p65 Binding Affinity

Cited 1 time

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The NF-κB p65 assay was performed according to a previously established protocol^{15 (link)}. Nuclear extracts were prepared from HeLa cells and the Transcription Assay System (Pierce Biotechnology, Rockford, IL) was used to evaluate the binding affinity of the NF-κB subunit p65 to the biotinylated consensus sequence. Luminescence was detected using a Fluostar Optima plate reader (BMG Labtech Inc, Durham, NC). Rocaglamide (Enzo Life Sciences, Inc., Farmingdale, NY, USA) was used as a positive control.

Acuña U.M., Ezzone N., Rakotondraibe L.H, & Carcache de Blanco E.J. (2021). Activity in MCF-7 Estrogen-Sensitive Breast Cancer Cells of Capsicodendrin from Cinnamosma fragrans. Anticancer research, 41(12), 5935-5944.

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NF-κB Transcription Assay Protocol

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The NF-κB assay was carried out according to established protocol.⁴ In brief, a nuclear extract was prepared from HeLa cells. The Transcription Assay System (Pierce Biotechnology, Rockford, IL) was used to evaluate the binding affinity to a biotinylated consensus sequence of the NF-κB subunit p65. Luminescence was detected using Fluostar Optima plate reader (BMG Labtech Inc, Durham, NC). Rocaglamide was used as a positive control.

Acuña U.M., Zi J., Orjala J, & Carcache de Blanco E.J. (2015). Ambiguine I Isonitrile from Fischerella ambigua Induces Caspase-Independent Cell Death in MCF-7 Hormone Dependent Breast Cancer Cells. International journal of cancer research, 49(1), 1655-1662.

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