Mitomycin C, Genticin, Penicillin, Streptomycine, Neomycin, M16 and M2 media, Plasmid purification kit, Tyrodes acid and leukemia Inhibitory Factor were purchased from Sigma (Germany). PMSG (Folligon) and hCG (Chorulon) were purchased from MSD Animal Health (New Zealand). DMEM, FCS and trypsin was obtained from Gibco BRL (France). RNA and DNA extraction kits were bought from Intron (Korea). Endofree Plasmid Maxi Kit was purchased from Qiagene (USA). High Pure PCR Purification Kit was from Roche (Germany). EW and R1 embryonic stem cells and pEGFP-C1 were kindly donated by Dr. Karim Nayernia at Institute of Molecular Medicine and Cell Therapy, Düsseldorf, Germany and GENEOCELL, Canada.
To generate knockout mouse, ES cells were cultured and electroporated with the gene targeting vector. The genetically-modified cells were selected and transferred into embryos. The modified ES cells along with the cells of embryo were used to produce chimeric mice. Crosses between the chimeras and wild type mice were resulted heterozygous offspring.
To generate knockout mouse, ES cells were cultured and electroporated with the gene targeting vector. The genetically-modified cells were selected and transferred into embryos. The modified ES cells along with the cells of embryo were used to produce chimeric mice. Crosses between the chimeras and wild type mice were resulted heterozygous offspring.