Five human CC cell lines (HPV 16-positive SiHa and CaSki cells, HPV 18-positive HeLa and SW756 cells, and HPV-negative C33A cells) were obtained from the American Type Culture Collection (Manassas, VA, USA). All cell lines were authenticated using short tandem repeat (STR) profiling. All mycoplasma-free cells were cultured at 37 °C in a humidified incubator with 5% CO 2 and grown in Eagle's minimum essential medium (EMEM; Siha, HeLa and C33A cells), RPMI-1640 medium (Caski cells), or Leibovitz's L-15 medium (SW756 cells) (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 2 mM L-glutamine, penicillin (100 units/ml), and streptomycin (100 µg/ml).
Hpv 16 positive siha and caski cells
Manufactured by American Type Culture Collection
Sourced in United States
HPV 16-positive SiHa and CaSki cells are human cervical cancer cell lines derived from cervical carcinomas. These cell lines are positive for human papillomavirus type 16 (HPV 16) and are commonly used in research related to cervical cancer and HPV.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
C33a cells, by American Type Culture Collection (1 mentions)
Leibovitz s l 15 medium, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
3 protocols using hpv 16 positive siha and caski cells
1
Culturing Human Cervical Cancer Cell Lines
2
Culturing Human Cervical Cancer Cell Lines
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Five human CC cell lines (HPV 16-positive SiHa and CaSki cells, HPV 18-positive HeLa and SW756 cells, and HPV-negative C33A cells) were obtained from the American Type Culture Collection (Manassas, VA, USA). All cell lines were authenticated using short tandem repeat (STR) pro ling. All mycoplasma-free cells were cultured at 37°C in a humidi ed incubator with 5% CO 2 and grown in Eagle's minimum essential medium (EMEM; Siha, HeLa and C33A cells), RPMI-1640 medium (Caski cells), or Leibovitz's L-15 medium (SW756 cells) (Invitrogen; Thermo Fisher Scienti c, Inc.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scienti c, Inc.), 2 mM L-glutamine, penicillin (100 units/ml), and streptomycin (100 μg/ml).
3
Establishment and Characterization of Cervical Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Five human CC cell lines (HPV 16-positive SiHa and CaSki cells, HPV 18-positive HeLa and SW756 cells, and HPV-negative C33A cells) were obtained from the American Type Culture Collection (Manassas, VA, USA). All cell lines were authenticated using short tandem repeat (STR) profiling. All mycoplasma-free cells were cultured at 37 °C in a humidified incubator with 5% CO2 and grown in Eagle’s minimum essential medium (EMEM; Siha, HeLa and C33A cells), RPMI-1640 medium (Caski cells), or Leibovitz’s L-15 medium (SW756 cells) (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.), 2 mM L-glutamine, penicillin (100 units/ml), and streptomycin (100 μg/ml).
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