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Cba array software

Manufactured by BD
Sourced in United States

CBA array software is a tool designed for the analysis and visualization of data from cell-based assays (CBA). The software provides functionality for processing, analyzing, and presenting results from CBA experiments.

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2 protocols using cba array software

1

Cytokine Profiling of Splenocyte-A20 Co-culture

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Splenocyte/A20 cultures were incubated at 37°C and 5% CO2 for 72 h. Culture supernatant was collected and secreted IFN-γ, TNF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, and IL-13 cytokines assayed using the mouse cytometric bead array flex kit (BD Biosciences, USA) following the manufacturer's protocol. Samples were analyzed using a FACSArray instrument (BD Biosciences, USA) using the CBA array software (BD Biosciences, USA).
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2

Cytokine Secretion Profiling in Splenocyte/A20 Co-cultures

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Splenocyte/A20 cultures were incubated at in 96-well U-bottom plates in 200 μl of complete media at 37°C and 5% CO2 for 72 h. Culture supernatant was collected and stored at −80°C prior to assay. Secreted IFN-γ, TNF, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12p70, and IL-13 cytokines were analyzed using the mouse cytometric bead array flex kit (BD Biosciences, USA) following the manufacturer's protocol. Samples were acquired using a FACSArray instrument (BD Biosciences, USA) and data analyzed using the CBA array software (BD Biosciences, USA).
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