To induce effector T cells and Tregs in vitro, naïve CD4+ or CD8+ T cells (1×105/well) were isolated from wild type, GPR41 (−/−), and GPR43(−/−) mice and activated with plate-coated anti-CD3 (5 μg/ml) and soluble anti-CD28 (2 μg/ml) in 96-well plates for 5-6 days in the presence or absence of Sodium Acetate (C2), Sodium Propionate (C3), or Sodium Butyrate (C4) at indicated concentrations. In some experiments where low TCR activation is required, the anti-CD3 was used at 1 μg/ml. RPMI 1640 medium (10% FBS) containing rapamycin (25 nM, Enzo), metformin (1 mM), or Trichostatin (TSA, 10 nM, Enzo) were used for the T cell culture. For Th17/Tc17 cells, hTGF-β1 (5 ng/ml), mIL-6 (20 ng/ml), mIL-1β (10 ng/ml), mIL-23 (10 ng/ml), mIL-21(10 ng/ml), mTNF-α (20 ng/ml), anti-mIL-4 (11B11, 10 Qg/ml), and anti-mIFN-γ (XMG1.2, 10 Qg/ml) were used. For Th1/Tc1 cells, hIL-2 (100 U/ml), mIL-12 (10 ng/ml), and anti–mIL4 (10 Tg/ml) were used. For the non-polarized condition (Tnp), hIL-2 (100 U/ml) was used.
Trichostatin tsa
Manufactured by Enzo Life Sciences
Trichostatin (TSA) is a histone deacetylase (HDAC) inhibitor. It functions by inhibiting HDAC activity, which can lead to the accumulation of acetylated histones.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using trichostatin tsa
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In vitro Effector T Cell and Treg Induction
2
In vitro Effector T Cell and Treg Induction
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To induce effector T cells and Tregs in vitro, naïve CD4+ or CD8+ T cells (1×105/well) were isolated from wild type, GPR41 (−/−), and GPR43(−/−) mice and activated with plate-coated anti-CD3 (5 μg/ml) and soluble anti-CD28 (2 μg/ml) in 96-well plates for 5-6 days in the presence or absence of Sodium Acetate (C2), Sodium Propionate (C3), or Sodium Butyrate (C4) at indicated concentrations. In some experiments where low TCR activation is required, the anti-CD3 was used at 1 μg/ml. RPMI 1640 medium (10% FBS) containing rapamycin (25 nM, Enzo), metformin (1 mM), or Trichostatin (TSA, 10 nM, Enzo) were used for the T cell culture. For Th17/Tc17 cells, hTGF-β1 (5 ng/ml), mIL-6 (20 ng/ml), mIL-1β (10 ng/ml), mIL-23 (10 ng/ml), mIL-21(10 ng/ml), mTNF-α (20 ng/ml), anti-mIL-4 (11B11, 10 Qg/ml), and anti-mIFN-γ (XMG1.2, 10 Qg/ml) were used. For Th1/Tc1 cells, hIL-2 (100 U/ml), mIL-12 (10 ng/ml), and anti–mIL4 (10 Tg/ml) were used. For the non-polarized condition (Tnp), hIL-2 (100 U/ml) was used.
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