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Biotinylated anti human igg pab

Manufactured by Southern Biotech
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Biotinylated anti-human IgG (pAb) is a polyclonal antibody that has been conjugated with biotin. It is designed for use in various immunoassay and detection techniques.

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Lab products found in correlation

Streptavidin hrp, by R&D Systems (2 mentions) Rabbit anti hmg1 2 3 pab, by Santa Cruz Biotechnology (2 mentions) Protein a g plus agarose, by Santa Cruz Biotechnology (2 mentions) Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Horseradish peroxidase hrp linked anti rabbit igg pab, by Cell Signaling Technology (1 mentions)

2 protocols using biotinylated anti human igg pab

1

Immunoprecipitation of rTim-3Fc Protein

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5 μg of recombinant mouse Tim-3 human IgG1 chimeric protein (rTim-3Fc) (R&D Systems, Minneapolis, MN) was added to 500 μL supernatant and immunoprecipitated with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Dallas, TX). Proteins were resolved, western blotted, and incubated with rabbit anti-HMG1/2/3 (pAb) (Santa Cruz Biotechnology), biotinylated anti-human IgG (pAb) (SouthernBiotech, Birmingham, AL), HRP-linked anti-rabbit IgG (pAb) (Cell Signaling Technology, Danvers, MA), and streptavidin-HRP (R&D Systems) followed by visualization with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rochester, NY).
Dolina J.S., Braciale T.J, & Hahn Y.S. (2014). Liver-primed CD8+ T cells suppress antiviral adaptive immunity through galectin-9-independent T-Cell immunoglobulin and mucin 3 engagement of high-mobility group box 1 in mice. Hepatology (Baltimore, Md.), 59(4), 1351-1365.
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2

Immunoprecipitation and Immunoblotting of Tim-3

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We added 5μg of recombinant (r) mouse Tim-3 human immunoglobulin G (IgG)1 chimeric protein (rTim-3Fc; R&D Systems, Minneapolis, MN) to 500 μL of supernatant and immunoprecipitated with Protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Dallas, TX). Proteins were resolved, western blotted, and incubated with rabbit anti-HMG1/2/3 (pAb; Santa Cruz Biotechnology), biotinylated anti-human IgG (pAb; SouthernBiotech, Birmingham, AL), horseradish peroxidase (HRP)-linked anti-rabbit IgG (pAb; Cell Signaling Technology, Danvers, MA), and streptavidin-HRP (R&D Systems), followed by visualization with SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rochester, NY).
Dolina J.S., Braciale T.J, & Hahn Y.S. (2014). Liver-primed CD8+ T cells suppress antiviral adaptive immunity through galectin-9-independent T-Cell immunoglobulin and mucin 3 engagement of high-mobility group box 1 in mice. Hepatology (Baltimore, Md.), 59(4), 1351-1365.
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