Cas block

Manufactured by Abcam

The CAS-block is a laboratory equipment designed to provide a controlled temperature environment for various applications. It serves as a heating block for incubating and maintaining sample temperatures during experimental procedures. The core function of the CAS-block is to ensure consistent and reliable temperature regulation for your samples.

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Lab products found in correlation

Cas block, by Thermo Fisher Scientific (3 mentions) Vt1000, by Leica (1 mentions) A12381, by Thermo Fisher Scientific (1 mentions) Alexa fluor conjugated phalloidin, by Thermo Fisher Scientific (1 mentions) Cas block, by Cell Signaling Technology (1 mentions) Ab14955, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Prolong gold, by Thermo Fisher Scientific (1 mentions) A11001, by Thermo Fisher Scientific (1 mentions) Dm5500b microscope, by Leica (1 mentions) Glial fibrillary acidic protein (gfap), by Abcam (1 mentions) Dapi counterstain, by Vector Laboratories (1 mentions) Fitc conjugated fluorescent secondary antibody, by Abcam (1 mentions) Paraformaldehyde (pfa), by Thermo Fisher Scientific (1 mentions)

4 protocols using cas block

Immunostaining of GAD65+67 and Somatostatin in Mouse Brain

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Nine-week-old female mice were deeply anesthetized with ketamine-xylazine followed by cold perfusion to the heart of PBS and 4% PFA. The removed brain was kept overnight at 4°C in 4%–4% PFA. The next day, the brain was washed in PBS (three times, 20 min each) before thin 40 µm coronal slices were made using a vibratome (Leica VT1000S). Slices were briefly washed with CAS-BLOCK (Life Technologies) before being incubated in CAS-BLOCK (300 µl) overnight with GAD65+67 antibodies (ab183999 Abcam, 1:1000) or Somatostatin (BMA Biomedicals Peninsula Laboratories T-4102, 1:100). The slices were then washed in PBS (three times, 20 min each) and incubated with CAS-BLOCK and secondary antibodies (Abcam ab150063, 1:500) for 3 hr. Antifade Mounting Medium (VECTASHIELD) was applied to prevent slice bleaching.

Matityahu L., Malgady J.M., Schirelman M., Johansson Y., Wilking J.A., Silberberg G., Goldberg J.A, & Plotkin J.L. (2022). A tonic nicotinic brake controls spike timing in striatal spiny projection neurons. eLife, 11, e75829.

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VE-Cadherin Immunostaining Protocol

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HUVEC in micropattern experiments were fixed for 10 min in 4% PFA, washed with PBS, then permeabilized for 20 min in 0.2% Triton X-100. Cells were blocked for 1 hr at RT with CAS-block (Life Technologies- 00-8120), then incubated in VE-cadherin primary antibody (1:100, Cell Signaling- D87F2) overnight in CAS-block at 4°C. Cells were washed in PBS, then incubated with Alexa-fluor-conjugated anti-species secondary antibodies (1:500) plus Alexa-fluor-conjugated phalloidin (1:250, Invitrogen- A12381) and DRAQ7 (1:1,000, Abcam- ab109202) in CAS-block for 3 hr at RT. For internalization experiments, after VE-cadherin labeling, internalization and subsequent fixation, HUVEC were incubated with Alexa-fluor-conjugated phalloidin (1:250, Invitrogen- A12381) plus Alexa-fluor-conjugated anti-mouse secondary antibody (1:500) and DRAQ7 in CAS-block for 3 hr at RT. Coverslips were mounted onto slides using Prolong Diamond antifade and sealed with nail polish.

Wylie L.A., Mouillesseaux K.P., Chong D.C, & Bautch V.L. (2018). Developmental SMAD6 Loss Leads to Blood Vessel Hemorrhage and Disrupted Endothelial Cell Junctions. Developmental biology, 442(2), 199-209.

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Immunostaining of Histone H3 in Tadpoles

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Fixed tadpoles were permeabilized by washing 3 × 15 min in PBS +0.01% Triton X-100 (PBT). Tadpoles were blocked for 1 h at room temperature in 10% CAS-block (Invitrogen #00–8120) in PBT. Then, tadpoles were incubated in primary antibody [1:1000 mouse anti-Histone H3 (phospho S10), Abcam ab14955] diluted in 100% CAS-block overnight at 4°C. Tadpoles were then washed 3 × 10 min at room temperature in PBT and blocked for 30 min in 10% CAS-block in PBT. Secondary antibody (goat anti-mouse 488, ThermoFisher A11001) was diluted 1:500 in 100% CAS-block and incubated for 2 h at room temperature. Tadpoles were then washed 3 × 10 min in PBT followed by a 10-min incubation in 1:2000 DAPI (Sigma D9542) before being washed with 1xPBS for 10 more minutes. Isolated tails were mounted on slides in ProLong Gold (ThermoFisher P36930). Images were acquired using a Leica DM 5500 B microscope using a 10X objective and processed using FIJI image analysis software.

Patel J.H., Ong D.J., Williams C.R., Callies L.K, & Wills A.E. (2022). Elevated pentose phosphate pathway flux supports appendage regeneration. Cell reports, 41(4), 111552.

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Dual Immunofluorescence and FISH Tissue Analysis

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Tissue microarray (TMA) paraffin sections were mounted on silanized
slides, baked for 5 minutes at 90 °C, and then de-paraffinized in
xylene. Slides were briefly dehydrated in 100% ethanol followed by 35
minutes in hot 1 mM EDTA. Sections were rinsed in dH₂O and
incubated with GFAP (Abcam) diluted in CAS Block (Invitrogen Corporation).
Slides were washed in 1X PBS and then incubated with FITC conjugated
fluorescent secondary antibody (Abcam) diluted in CAS Block. There is an
additional wash in 1XPBS followed by fixation in 4% paraformaldehyde (USB
Corporation) for 20 minutes. Slides were dehydrated in an ethanol series and
air dried. 20 μl of PTCH1/CEP9 (with GFAP) probe working solution
(Empire genomics) was applied to the hybridization area, a 24x50 mm
coverslip is placed over the top, and the edges of the coverslip were sealed
with a continuous bead of rubber cement. The slide and probe were
co-denatured at 95°C for 4 minutes and hybridized 24 hours or more at
37°C in a humidified chamber. Slides were then washed in 2X saline
sodium citrate buffer (SSC)/0.1%NP40 at 70°C for 1 minute and DAPI
counterstain (Vector Laboratories) was applied as well as a glass coverslip.
Visualization of the dual FISH/immunoflourescence signals was accomplished
by use of a fluorescent microscope with standard filters.

Yao M., Ventura P.B., Jiang Y., Rodriguez F.J., Wang L., Perry J.S., Yang Y., Wahl K., Crittenden R.B., Bennett M.L., Qi L., Gong C.C., Li X.N., Barres B.A., Bender T.P., Ravichandran K.S., Janes K.A., Eberhart C.G, & Zong H. (2020). Astrocytic trans-differentiation completes a multicellular paracrine feedback loop required for medulloblastoma tumor growth. Cell, 180(3), 502-520.e19.

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