8 ohdg assay kit

Malondialdehyde (MDA) and 8-hydroxy-2’-deoxyguanosine (8-OHdG) levels were analyzed using the MDA assay kit (Sigma-Aldrich, St. Louis, MO, USA) and the 8-OHdG assay kit (Abcam, Cambridge, MA, USA), respectively. Reduced glutathione (GSH) and oxidized glutathione (GSSG) levels were determined using the GSH detection kit (Enzo Life Sciences, Farmingdale, NY, USA). Activities of catalase and superoxide dismutase (SOD) were measured using commercial kits (Invitrogen, Carlsbad, CA, USA). All analyses were performed following the manufacturers’ instructions.

Levels of 8-OHdG, an RNA nucleoside that is an oxidized derivative of guanosine, were quantified using the 8-OHdG Assay Kit (Abcam, Inc., UK) following to the manufacturer's instructions. The absorbance of each treatment group was determined at 450 nm, and results were presented as ng of 8-OHdG/ml.

An automatic analyzer (Hitachi, Osaka, Japan) was used for analyzing serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. Hepatic malondialdehyde (MDA) concentrations were assessed using the MDA assay kit (Sigma-Aldrich, St. Louis, MO, USA). Serum 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels were analyzed using the 8-OHdG assay kit (Abcam, Cambridge, MA, USA). Hepatic levels of GSH and oxidized GSH (GSSG) were assessed using the GSH detection kit (Enzo Life Sciences, Farmingdale, NY, USA). Serum levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β were determined using ELISA kits (R&D Systems, Minneapolis, MN, USA). Hepatic myeloperoxidase (MPO) activities were measured using the MPO activity assay kit (Abcam, Cambridge, MA, USA).