Frozen small intestinal tissue sections of IgER-TG mice were stained with PE-labeled mouse anti-human FcεRI (Cra1; eBioscience) and Alexa Fluor® 647labeled- anti mouse CD11c. Specificity of the staining was confirmed with appropriate isotype control antibodies and tissue from WT mice. Immunofluorescence histochemistry of human GI tissue was performed as described using discarded tissue of a published cohort 79 (link). All microscopy was performed at the Harvard Digestive Disease Center Core Facility.
Pe labeled mouse anti human fcεri cra1
Manufactured by Thermo Fisher Scientific
The PE-labeled mouse anti-human FcεRI (Cra1) is a monoclonal antibody that binds to the high-affinity IgE receptor (FcεRI) expressed on the surface of human mast cells and basophils. This antibody is conjugated with the fluorescent dye phycoerythrin (PE), allowing for the detection and analysis of FcεRI-positive cells through flow cytometry.
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2 protocols using pe labeled mouse anti human fcεri cra1
1
Quantifying FcεRI+ Myeloid Cells in Mouse and Human GI
2
Quantifying FcεRI+ Myeloid Cells in Mouse and Human GI
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Frozen small intestinal tissue sections of IgER-TG mice were stained with PE-labeled mouse anti-human FcεRI (Cra1; eBioscience) and Alexa Fluor® 647labeled- anti mouse CD11c. Specificity of the staining was confirmed with appropriate isotype control antibodies and tissue from WT mice. Immunofluorescence histochemistry of human GI tissue was performed as described using discarded tissue of a published cohort 79 (link). All microscopy was performed at the Harvard Digestive Disease Center Core Facility.
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