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1100 capillary hplc system

Manufactured by Agilent Technologies

The 1100 capillary HPLC System is a high-performance liquid chromatography (HPLC) instrument designed for analytical and preparative-scale separations. It features a capillary-scale flow path, enabling the use of small sample volumes and flow rates. The system is equipped with a variety of detectors and modules to support a range of HPLC applications.

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2 protocols using 1100 capillary hplc system

1

HPLC Analysis of Modified Placental DNA

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An Agilent 1100 capillary HPLC System equipped with a Synergi C18 analytical column was used for HPLC analysis of native and modified analog of human placental DNA. General chromatographic conditions were as follows: C18 column (2 mm × 150 mm with 4 μm particle size); eluant A, 5 mM aqueous ammonium acetate buffer, pH 7; eluant B, Acetonitrile (CH3N) gradient solution the CH3N concentration was raised from 0 to 4.0% in the first 5 min; from 4.0 to 6.5% over 30 min; held at 6.5% for 5 min, and then raised to 90% to wash residual material off the column at a constant flow rate of 500 μL/min. DNA bases were detected by diode array detector (DAD) at 254 nm, their absorption maximum. LC-MS analyzes of CEdG standard were carried out using a Micromass Quattro Ultima Triple Quadrupole Mass Spectrometer interfaced to an Agilent 1100 capillary HPLC System.
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2

Mass Spectrometry Analysis of Synthesized Compounds

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The synthesized compounds were analyzed using a Finnigan TSQ Quantum Discovery instrument (Thermo Electron, San Jose, CA) coupled to an Agilent 1100 Capillary HPLC system. The MS was operated in the negative ion ESI mode. A 250 × 10 mm, 5 μm, C18 Luna column and a Krudkatcher disposable precolumn filter (Phenomenex, Torrance, CA) were used. The column was eluted at 10 μL/min with a linear gradient of CH3CN in 15 mM NH4OAc. CH3CN was brought from 15% to 75% in 30 min, then the organic solvent was held at 75% for 5 min followed by a 14 min reequilibration at 15% CH3CN. The ESI source was set in the negative ion mode as follows: voltage 5 kV; current 50 μA; heated ion transfer tube 330 °C. The metabolites were measured by MS/MS using selected reaction monitoring (SRM) mode. The collision energy for all transitions was 15 eV, and the Ar collision gas pressure was 1.0 mTorr. The MS settings of the ion source were optimized using standard solutions of compounds 6 and 9, 7 and 10, and 8 and 11 to produce maximum MS/MS signals at m/z 356 → m/z 209, m/z 338 → 209 and m/z 354 → 225, respectively.
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