treated with 4% paraformaldehyde and stained with DAPI (4′,6-diamidino-2-phenylindole,
1.0 μg/mL) in the dark. DAPI stained cells were examined by
using a fluorescence confocal scanning microscope (Zeiss, Germany).21 (link)
The Fluorescence Confocal Scanning Microscope is a high-resolution imaging instrument that uses a focused beam of light to scan and capture images of a sample. It is designed to provide detailed, high-contrast images of fluorescently labeled specimens by selectively illuminating and detecting light from a specific focal plane within the sample.
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