DA and 5-HT contents in the dialysate fractions were analyzed by HPLC with electrochemical detection. Chromatography was performed using an Ultimate 3000 System (Dionex, Sunnyvale, CA, USA), a Coulochem III electrochemical detector (model 5300; ESA, Chelmsford, MA, USA) with 5020 guard cell, 5014B microdialysis cell, and Hypersil Gold-C18 analytical column (3 × 100 mm; Thermo Scientific, Waltham, MA, USA). The mobile phase was composed of 0.1 M potassium phosphate buffer adjusted to pH 3.6, 0.5 mM EDTA, 16 mg/L 1-octanesulfonic acid sodium salt, and 2% methanol. The flow rate during analysis was set at 0.7 mL/min. The applied potential was + 600 mV for the guard cell, and E1 = − 50 mV and E2 = + 300 mV for the microdialysis cells, with a sensitivity set at 50 nA/V. The chromatographic data was processed by Chromeleon v. 6.80 (Dionex) software, run on a personal computer.
5020 guard cell
Manufactured by Thermo Fisher Scientific
Sourced in United States
The 5020 guard cell is a laboratory equipment component designed to monitor and control the environment within an analytical system. It serves as a protective barrier, regulating the flow and composition of gases or liquids entering the system. The core function of the 5020 guard cell is to maintain the integrity and stability of the analytical conditions, ensuring reliable and accurate results.
Automatically generated - may contain errors
3 protocols using 5020 guard cell
1
Quantification of DA and 5-HT in Dialysate
2
HPLC Analysis of Neurotransmitters
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DA and 5-HT contents in the dialysate fractions were analyzed by HPLC with electrochemical detection. Chromatography was performed using an Ultimate 3000 System (Dionex, Sunnyvale, CA, USA), a Coulochem III electrochemical detector (model 5300; ESA, Chelmsford, MA, USA) with a 5020 guard cell, a 5014B microdialysis cell and a Hypersil Gold-C18 analytical column (3 × 100 mm; Thermo Scientific, Waltham, MA, USA). The mobile phase was composed of 0.1 M potassium phosphate buffer adjusted to pH 3.6, 0.5 mM EDTA, 16 mg/L 1-octanesulfonic acid sodium salt, and 2% methanol. The flow rate during analysis was set at 0.7 mL/min. The applied potential of a guard cell was + 600 mV, while those of microdialysis cells were: E1 = − 50 mV and E2 = + 300 mV with a sensitivity set at 50 nA/V. The chromatographic data was processed by Chromeleon v. 6.80 (Dionex) software, run on a personal computer.
3
HPLC-EC Analysis of DA and 5-HT
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DA and 5-HT contents in the dialysate fractions were analyzed by HPLC with electrochemical detection. Chromatography was performed using an Ultimate 3000 System (Dionex, Sunnyvale, CA, USA), a Coulochem III electrochemical detector (model 5300; ESA, Chelmsford, MA, USA) with a 5020 guard cell, a 5014B microdialysis cell and a Hypersil Gold-C18 analytical column (3 × 100 mm; Thermo Scientific, Waltham, MA, USA). The mobile phase was composed of 0.1 M potassium phosphate buffer adjusted to pH 3.6, 0.5 mM EDTA, 16 mg/L 1-octanesulfonic acid sodium salt and 2% methanol. The flow rate during analysis was set at 0.7 mL/min. The applied potential of a guard cell was + 600 mV, while those of the microdialysis cells were E1 = -50 mV and E2 = + 300 mV with a sensitivity set at 50 nA/V. The chromatographic data were processed by Chromeleon v. 6.80 (Dionex) software, run on a personal computer.
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