CFU assay was started at 48 h after gene editing. For each condition, 1.1 ml of semi-solid methylcellulose medium (StemCell Technologies) containing 300 cells and with 10 ng/ml IL-3 (+IL-3 treatment) or without IL-3 (−IL-3 treatment) were plated in a well of a SmartDish (StemCell Technologies) in duplicates. The cells were incubated at 37°C, 5% O2, and 5% CO2 for 14 d. The resulting progenitor colonies were counted and scored with STEMVision analysis (StemCell Technologies) as per the manufacturer’s instruction.
Stemvision analysis
Manufactured by STEMCELL
Sourced in United States
STEMVision is a versatile image analysis software designed for the quantification and analysis of cells and colonies. It offers a user-friendly interface and a range of advanced tools to support various cell-based assays and applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using stemvision analysis
1
CFU Assay for Progenitor Cell Analysis
2
Colony Forming Unit Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CFU assay was started at 72 h after editing. For each condition, 1.1 ml of semi-solid methylcellulose medium (StemCell Technologies) containing 500 cells was plated in a well of a SmartDish (StemCell Technologies) in duplicates. The cells were incubated at 37°C, 5% O2, and 5% CO2 for 14 d. The resulting progenitor colonies were counted and scored with STEMVision analysis (StemCell Technologies) as per the manufacturer’s instruction. Colonies were picked and subjected to sequencing. Shortly, colonies were washed with PBS and then resuspended in 25 µl DNA-QuickExtract solution. PCRs were performed and sent for Sanger sequencing.
3
Colony Forming Unit Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CFU assay was started at 48 h post-genome editing. For each condition, 1.1 mL of semi-solid methylcellulose medium (StemCell Technologies, Seattle, WA, USA) containing 300 cells was plated in a well of a SmartDish (StemCell Technologies, Seattle, WA, USA) in duplicates and then the cells were incubated at 37°C, 5% O2, and 5% CO2 for 14 days. The resulting progenitor colonies were counted and scored with STEMVision analysis (StemCell Technologies, Seattle, WA, USA) following the manufacturer’s instructions.
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