Responsiveness to an inescapable stressful situation was measured using the TST test. Mice were suspended by the tail to a hook using adhesive tape. The time spent immobile, thought to reflect a resignation state, was recorded during a 6-min session through an automated device (Bioseb). Mice which climbed their tail were removed from analysis.
Automated device
Manufactured by Bioseb
Sourced in France
The Automated device is a piece of laboratory equipment designed to perform automated tasks. It features programmable functionality to execute various operations with precision and consistency. The core function of this device is to automate repetitive or complex procedures, thereby improving efficiency and reliability in the laboratory setting.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using automated device
1
Tail Suspension Test for Stress Resilience
2
Evaluating Antidepressant-like Effects of G. japonicum Extract
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To verify whether G. japonicum extract has antidepressant-like effects, the TST immobility was performed 24 hours after PAT. The mice were suspended by the tail using an adhesive tape attached to a hook connected to a strain gauge. The automated device (BioSeb, Chaville, France) was used to score immobility during the last 6 min.
3
Behavioral Assessments of Depressive Symptoms
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Depressive behaviors were assessed by the tail suspension test (TST), forced swim test (FST) and the sucrose intake test (SIT). The protocol procedures of behavioral tests were referred to previous works [20 (link)]. Briefly, TST was performed as the tails of mice were wrapped with tape from the base to the end and placed upside down on a hook using tape. The time when the mice last did not actively try to escape was quantified using an automated device (BioSeb, Chaville, France). FST was performed 24 h after the TST as described previously. In brief, each mouse was placed individually in a transparent cylinder (height: 25 cm; diameter: 15 cm) filled with water. An analysis of immobility and activity time in the last 4 min was conducted using SMART v3.0 software (Panlab SL, Barcelona, Spain) after each mouse had swum for 6 min. SIT measured 1% sucrose intake for 1 h between 19:00 and 20:00 every Monday. Prior to the SIT, all mice were denied water and food. After the test, the mice were allowed to eat and drink freely.
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