Anti tubulin antibody t6074

Manufactured by Merck Group

Anti-tubulin antibody (T6074) is a laboratory reagent used in various biochemical and cell biology applications. It is a highly specific antibody that binds to tubulin, a key structural protein found in the cytoskeleton of cells. The antibody can be used to detect and visualize tubulin in samples, such as in immunofluorescence microscopy or Western blotting experiments.

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Anti-tubulin (542 citations) T6074 (118 citations) Anti-tubulin antibody (103 citations) Anti-α-tubulin antibody (T6074; (5 citations) Anti-tubulin (#T6074, (3 citations)

3 protocols using anti tubulin antibody t6074

Molecular Mechanisms of Cell Differentiation

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C2C12, 3T3-L1, and HEK293 cells were purchased from ATCC and maintained as instructed. Differentiation of C2C12 was performed as reported (Burattini et al., 2004 (link)). Transfection in C2C12 was performed using DharmaFECT1 as instructed (Thermo Scientific). 7,8-DHF was purchased from Tokyo Chemical Industry. HFD (40% kcal) was obtained from Research Diet. Human insulin was obtained from Eli Lilly. Other chemicals were purchased from Sigma-Aldrich. Antibodies against pAkt S473, IR, pIR Y1150/1151 (#3024), pAMPKa T172 (#2535), AMPKα (#2603), pACC S79 (#3661), ACC (#3676), pERK T202/ Y204 (#9106), ERK (#9102), pCREB S133 (#9198), CREB (#4820), and TrkB (4603) were purchased from Cell Signaling. Anti-Akt1 (sc5298) and anti-pTrkB Y706 (sc135645) antibodies were obtained from Santa Cruz Biotechnology. Anti-UCP1 antibody (ab155117) was obtained from Abcam. Anti-tubulin antibody (T6074) was obtained from Sigma-Aldrich.

Chan C.B., Tse M.C., Liu X., Zhang S., Schmidt R., Otten R., Liu L, & Ye K. (2015). Activation of Muscular TrkB by its Small Molecular Agonist 7,8-Dihydroxyflavone Sex-Dependently Regulates Energy Metabolism in Diet-Induced Obese Mice. Chemistry & biology, 22(3), 355-368.

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Investigating BDNF Signaling in C2C12 Cells

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C2C12 cells were purchased from the American Type Culture Collection (ATCC; Manassas, VA) and maintained as instructed by ATCC. Transfection of C2C12 cells was performed using DharmaFECT1 (Thermo Fisher Scientific, Waltham. MA) according to manufacturer’s instructions. Recombinant human BDNF and anti-BDNF antibody were purchased from Abcam (Cambridge, UK). Human insulin was obtained from Elli Lily and Company (Indianapolis, IN). All other chemicals were purchased from Sigma-Aldrich (St. Louis MO). Antibodies against pAkt S473 (#9271), pAMPKα T172 (#2535), AMPKα (#2603), pACC S79 (#3661), ACC (#3676), pCREB S133 (#9198), CREB (#4820), and TrkB (#4603) were purchased from Cell Signaling Technology (Danvers, MA). All autophagy and mTOR signaling pathway antibodies were also obtained from Cell Signaling Technology. Anti-Akt1 (sc5298), anti-IRβ (sc53742), and anti-pTrkB Y706 (sc135645) antibodies were obtained from Santa Cruz Biotechnology (Dallas, TX). Anti-PPARα (#8934) and PGC-1α (#54481) were obtained from Abcam while anti-tubulin antibody (T6074) was obtained from Sigma-Aldrich. [1-¹⁴C]-Palmitic acid was purchased from PerkinElmer (Waltham, MA). Adenoviruses that express shBdnf were synthesized by Cyagen (Santa Clara, CA) based on a previously reported sequence (63 (link)).

Yang X., Brobst D., Chan W.S., Tse M.C., Herlea-Pana O., Ahuja P., Bi X., Zaw A.M., Kwong Z.S., Jia W.H., Zhang Z.G., Zhang N., Chow S.K., Cheung W.H., Louie J.C., Griffin T.M., Nong W., Hui J.H., Du G.H., Noh H.L., Saengnipanthkul S., Chow B.K., Kim J.K., Lee C.W, & Chan C.B. (2019). Muscle-generated BDNF is a sexually dimorphic myokine that controls metabolic flexibility. Science signaling, 12(594), eaau1468.

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Immunoblotting of Inflammasome Proteins

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Anti-phospho PKD #2051, anti-PKD3 (D57E6) #5655, anti-PKD2 (D1A7) #8188, anti-human caspase-1 (D7F10) #3866, anti-cleaved Gasdermin-D (E7H9G) #36425, were from Cell Signaling Technologies. The anti-tubulin antibody (#T6074) was from Sigma. The anti-ASC antibody coupled to PE (#653904) used for ASC speck analysis by flow cytometry was from Biolegend.

Heiser D., Rubert J., Unterreiner A., Maurer C., Kamke M., Bodendorf U., Farady C.J., Roediger B., & Bornancin F. (2021). Evaluation of protein kinase D auto-phosphorylation as biomarker for NLRP3 inflammasome activation.

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