Analytical grade solvents were used. Reagents and 2-hydroxypropyl-β-cyclodextrin (2HP-β-CD ) were purchased from Sigma-Aldrich or Acros Chemical Co. Ltd., and β-Lapachone was synthesized following a previously reported procedure [38 (link)] (Fig 1
2 hydroxypropyl β cyclodextrin 2hp β cd
Manufactured by Merck Group
Sourced in United States
2-hydroxypropyl-β-cyclodextrin (2HP-β-CD) is a chemically-modified cyclodextrin molecule. Cyclodextrins are a class of cyclic oligosaccharides that can form inclusion complexes with a variety of guest molecules. 2HP-β-CD is a water-soluble derivative of the naturally-occurring β-cyclodextrin.
Automatically generated - may contain errors
Lab products found in correlation
Merck silica gel 60 f254 plates, by Merck Group (1 mentions)
Ir 780 iodide, by Merck Group (1 mentions)
Resiquimod r848, by Selleck Chemicals (1 mentions)
Polyvinyl alcohol (pva), by Merck Group (1 mentions)
Hoechst 33342, by Yeasen (1 mentions)
Lysotracker red dnd 99, by Yeasen (1 mentions)
Tenofovir, by Biosynth (1 mentions)
Sodium dodecyl sulphate (sds), by Merck Group (1 mentions)
Mw 1450, by Merck Group (1 mentions)
Tert amyl alcohol, by Merck Group (1 mentions)
Tert butanol, by Merck Group (1 mentions)
4 protocols using 2 hydroxypropyl β cyclodextrin 2hp β cd
1
Synthesis and Purification of β-Lapachone
2
Resiquimod-loaded PLGA Nanoparticle Synthesis
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Resiquimod (R848) was purchased from Selleck (Houston, TX, USA). Poly(D, L-lactic-co-glycolic acid) (PLGA) (LA: GA = 50:50, 10–20 kDa) and cell counting kit-8 (CCK-8) were purchased from MedChenExpress (Monmouth Junction, NJ, USA). Poly(vinyl alcohol) (PVA), the heptamethine indocyanine dye IR-780 iodide, and 2-hydroxypropyl-β-cyclodextrin (2-HP-β-CD) were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). The anti-mouse TNFR2 (CD120b) antibody was purchased from Bio X Cell (West Lebanon, NH). Hoechst 33,342 and LysoTracker Red DND-99 were purchased from Yeasen Biotechnology (Shanghai, China). All other reagents used were of the highest commercially available grade.
3
Formulation and Characterization of Antiviral Vaginal Gels
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Tenofovir (TFV, lot: FT104801501) was provided by Carbosynth Limited (Berkshire, UK). Dapivirine (DPV; lot: 60416PIL04) was kindly provided by the International Partnership for Microbicides (IPM, Silver Spring, MD, USA). Hydroxypropylmethyl cellulose—Methocel® K4M (HPMC; lot: SK27012N11) was kindly supplied by Colorcon Ltd. (Kent, UK). 2-Hydroxypropyl-β-cyclodextrin (2HPβCD; lot: BCBQ9423V), Polysorbate—Tween® 60 (P60; lot: MKBT3178V), and sodium dodecyl sulphate (SDS; lot: STBJ1530) were acquired from Sigma–Aldrich (St. Louis, MO, USA). Technical-grade water (lot: 9V011540) was acquired from Panreac (Barcelona, Spain).
All other reagents in this study were of analytical grade and were used without further purification. Demineralised water was used in all cases, except in the preparation of the gels, when technical-grade water was used.
All other reagents in this study were of analytical grade and were used without further purification. Demineralised water was used in all cases, except in the preparation of the gels, when technical-grade water was used.
4
Formulating Peptides for NOD Mice
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Three peptides, which have relevance to type 1 diabetes in the non obese diabetic (NOD) mouse model of type 1 diabetes, with different hydrophobicities were used in the study: WE14 (WSRMDQLAKELTAE), a peptide of chromogranin A, m31 (YVRPLWVRME), a mimotope that stimulates highly diabetogenic CD4 T lymphocytes in the NOD mouse [23] , and pro-insulin B9-23 (SHLVEALYLVCGERG) (GL Biochem, Shanghai, China). The solubility of these peptides was tested in water, acetic acid, tert-butanol and tert-amyl alcohol (Sigma-Aldrich, Gillingham, UK). After determining the best combination of solvents for all three peptides, 10 µg of peptide was further formulated with 1 µg of the following excipients to improve the MN coating efficiency and assist dissolution in the skin: poly vinyl alcohol MW2000 (PVA 2000), polyethylene glycol MW1450 (PEG 1450), pluronic 127 (PF127), pluronic 68 (PF68) or 2-hydroxypropyl-β-cyclodextrin (2HPβCD) (Sigma-Aldrich, Gillingham, UK).
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