Bms433771

Manufactured by Merck Group

Sourced in Germany

BMS433771 is a piece of laboratory equipment developed by Merck Group. It is designed for use in scientific research and analysis. The core function of BMS433771 is to perform specific tasks required in a laboratory setting, but a detailed description cannot be provided while maintaining an unbiased and factual approach.

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Lab products found in correlation

4 protocols using bms433771

Compound Preparation for Cell Assay

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CPM (S1146) was purchased from Selleck Chemicals. RSV604 (SML1695) and BMS433771 (BMS0022) were purchased from Sigma–Aldrich. ALS8176 (HY–12983A), GS5806 (HY–16727), and Ziresovir (HY–109142) were purchased from MedChem Express. Compounds were solubilized at 8 mM in 100% v/v DMSO, then serially diluted to the desired concentrations in DMEM with 2% fetal bovine serum (FBS) and 0.25% v/v DMSO in the assay.

Gao Y., Cao J., Xing P., Altmeyer R, & Zhang Y. (2021). Evaluation of Small Molecule Combinations against Respiratory Syncytial Virus In Vitro. Molecules, 26(9), 2607.

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Protein-Anhydride Interactions for Biomedical Applications

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3-hydroxyphthalic anhydride (HP), maleic anhydride (ML), succinic anhydride (SU), human serum albumin (HSA), chicken ovalbumin (OVA), bovine serum albumin (BSA), and β-lactoglobulin (β-LG) were purchased from Sigma (St. Louis, MO, USA). Cell Counting Kit-8 (CCK-8) was bought from Dojindo Molecular Technologies, Inc. (Dojindo, Japan). 2,4,6-trinitrobenzenesulfonic acid (TNBS) was purchased from Sigma. ρ-hydroxyphenylglyoxal (ρ-HPG) was purchased from Fisher Scientific Co. (Valley Park, VA, USA). BMS433771 and TMC353121 were purchased from Sigma. Anti-RSV antibody (NCL-RSV3) was from Leica Biosystems (Leica Biosystems Newcastle Ltd., Newcastle Upon Tyne, UK). Rabbit anti-mouse HRP antibody was purchased from Dako (Dako, Glostrup, Denmark). Low melting point agarose was bought from Invitrogen (Invitrogen, Carlsbad, CA, USA).

Sun Z., Wang Q., Jia R., Xia S., Li Y., Liu Q., Xu W., Xu J., Du L., Lu L, & Jiang S. (2015). Intranasal Administration of Maleic Anhydride-Modified Human Serum Albumin for Pre-Exposure Prophylaxis of Respiratory Syncytial Virus Infection. Viruses, 7(2), 798-819.

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Preparation of Lentiviral Pseudotypes with Diverse Envelope Proteins

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Lentiviral pseudotypes were prepared as described previously (16 (link)). The viral plasmids used consisted of (i) a packaging plasmid containing the HIV gag-pol genes (pRV8.74) (33 (link)); (ii) a transfer plasmid containing a firefly luciferase reporter gene flanked by lentiviral long terminal repeats (pWPI-F-Luc-BLR) (34 (link)); and (iii) an envelope plasmid coding the RSV glycoprotein (hRSV-F), Synagis- (K272E) or fusion inhibitor (K394R)-resistant F-mutations (16 (link)), VSV-G (pczVSV-G) (35 (link)), rabies virus (RABV) glycoprotein (36 (link)), or empty vector control (pcDNA3.1). Synagis (Palivizumab; AbbVie, Wiesbaden, Germany) and BMS-433771 (Sigma-Aldrich, St. Louis, MO, USA) were used as controls.

Sake S.M., Kosch C., Blockus S., Haid S., Gunesch A.P., Zhang X., Friesland M., Trummer S.B., Grethe C., Kühnel A., Rückert J., Duprex W.P., Huang J., Rameix-Welti M.A., Empting M., Fischer N., Hirsch A.K., Schulz T.F, & Pietschmann T. (2022). Respiratory Syncytial Virus Two-Step Infection Screen Reveals Inhibitors of Early and Late Life Cycle Stages. Antimicrobial Agents and Chemotherapy, 66(12), e01032-22.

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Screening Antiviral Compounds against RSV

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HEp-2 and HEK293T cells were purchased from the American Type Culture Collection (ATCC) and maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 2 mM l-glutamine. RSV A2 was obtained from Wuhan University, China. RSV A2-F_K394R was isolated and identified in our previous study (29 (link)). All the viruses were grown in HEp-2 cells and stored at −70°C. Viral titers were determined by plaque assay. Salvianolic acid R (LF-6) was isolated from the aerial part of Mesona chinensis Benth. (Lamiaceae) by the authors, following our previously reported procedure (30 (link)). The purity of LF-6 (>98%) was validated through high-performance liquid chromatography (HPLC) analysis. Ribavirin, heparin, and BMS-433771 were purchased from Sigma-Aldrich, Inc. TMC-353121, JNJ-53718678, GS-5806, and AK-0529 were purchased from MedChem Express, Inc.

Tang W., Li Y., Song Q., Wang Z., Li M., Zhang Q., Wang Y., Ye W, & Li Y. (2021). Mechanism of Cross-Resistance to Fusion Inhibitors Conferred by the K394R Mutation in Respiratory Syncytial Virus Fusion Protein. Journal of Virology, 95(20), e01205-21.

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