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Perilipin

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Perilipin is a lipid droplet-associated protein that regulates the storage and mobilization of cellular lipids. It serves as a core structural component of lipid droplets and plays a crucial role in the regulation of lipolysis.

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Lab products found in correlation

Ab28364, by Cedarlane (2 mentions) Cl8942ap, by Cedarlane (2 mentions) Lsm 510 confocal microscope, by Zeiss (2 mentions) Ab13970, by Abcam (2 mentions) Fluorescently labeled secondary antibody, by Thermo Fisher Scientific (1 mentions) Vldlr, by Abcam (1 mentions) Cd11b, by Thermo Fisher Scientific (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) Srebp1, by Santa Cruz Biotechnology (1 mentions) Pparγ, by Santa Cruz Biotechnology (1 mentions) Glut4, by Santa Cruz Biotechnology (1 mentions) Alkaline phosphatase, by Jackson ImmunoResearch (1 mentions) Horseradish peroxidase hrp, by Jackson ImmunoResearch (1 mentions) Anti caspase 3, by Cell Signaling Technology (1 mentions) Nf kb, by Santa Cruz Biotechnology (1 mentions) Hsp70, by Stressgen (1 mentions) Adipophilin, by Biosynth (1 mentions)

Close spelling variants of this product

Anti-perilipin (3 citations) Perilipin 1 (2 citations)

4 protocols using perilipin

1

Immunohistochemical Analysis of Invasive Breast Cancer

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Patient slices were obtained from the National Disease Research Interchange. All patients were diagnosed with invasive lobular/ductal carcinoma breast, Grade 2. Patients received total mastectomy or simple radical mastectomy with no known presurgical therapy. Samples were analyzed under the approved University of Texas Southwestern Medical Center Institutional Review Board protocol # STU 012017-043 (Endotrophin Upregulation in Tumor Tissues). Mouse tissues were dissected and fixed in 10% formalin overnight. Paraffin processing, embedding, sectioning and hematoxylin/eosin staining and trichrome staining were performed by John Sheldon at UTSW Medical Center. Immunostaining was performed following standard protocols, with the following antibodies: Perilipin (1:1000, #20R-PP004, Fitzgerald), EGFP (1:500, ab13970, Abcam), α-SMA (1:500, eBioscience™, #14-9760-80), CD31 (1:200, Abcam, #ab28364), Mac2 (1:750, CL8942AP, CEDARLANE) and collagen hybridizing peptide (#5276, Advanced BioMatrix). Images were acquired with LSM510 confocal microscope (Zeiss) and analyzed by Image J software.
Zhu Q., Zhu Y., Hepler C., Zhang Q., Park J., Gliniak C., Henry G.H., Crewe C., Bu D., Zhang Z., Zhao S., Morley T., Li N., Kim D.S., Strand D., Deng Y., Robino J.J., Varlamov O., Gordillo R., Kolonin M.G., Kusminski C.M., Gupta R.K, & Scherer P.E. (2022). Adipocyte mesenchymal transition contributes to mammary tumor progression. Cell reports, 40(11), 111362.
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2

Immunostaining of Whole-mounted EATs

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Whole-mounted EATs were incubated with primary antibodies against VLDLR (1:500 dilution; Abcam, #92943, Cambridge, MA), ceramide (1:500 dilution; Sigma-Aldrich, #C8104, St. Louis, MO), perilipin (1:1000; Fitzgerald, #20R-PP004, Stow, MA), CD11b (1:1000), and CD11c (1:1000; eBioscience, #E01079, #4290718, San Jose, CA). After incubation with fluorescently labeled secondary antibodies (Thermo Fisher Scientific, Waltham, MA) and 4′,6-diamidino-2-phenylindole (DAPI; Vector Laboratory, Burlingame, CA) staining, samples were examined under a Zeiss LSM 700-NLO confocal microscope.
Shin K.C., Hwang I., Choe S.S., Park J., Ji Y., Kim J.I., Lee G.Y., Choi S.H., Ching J., Kovalik J.P, & Kim J.B. (2017). Macrophage VLDLR mediates obesity-induced insulin resistance with adipose tissue inflammation. Nature Communications, 8, 1087.
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3

Western Blot Antibody Characterization

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For Western, the following antibodies were used: adipophilin (1:10,000, guinea pig) and perilipin (1:100,000, guinea pig) from Fitzgerald Industries International (Acton, MA, USA); CHOP (1:200, rabbit), SREBP1 (1:200, mouse), NFkB (1:200, mouse), PPARγ (1:200, rabbit), GLUT4 (1:200, mouse) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-KDEL (BiP, 1:2500, mouse) from MBL International (Woburn, MA, USA); anti-caspase 3 (1:1000, rabbit) from Cell Signaling Technology (Danvers, MA); FAS (1:3000, mouse) from BD Transduction Laboratories (Franklin Lakes, NJ, USA); actin (1:10,000, rabbit), and polyubiquitin (1:500, rabbit) from Sigma-Aldrich; Hsp70 (1:1000, mouse) from Stressgen Bioreagents (Victoria, BC, Canada), while anti-β1 (1:5000 rabbit) and anti-S4 (1:2000, rabbit) antibodies were custom obtained 24 (link). Secondary antimouse, anti-rabbit and anti-guinea pig Fab' fragments labelled with horseradish peroxidase (HRP) or alkaline phosphatase (AP) were from Jackson Immunoresearch (West Grove, PA, USA).
Wójcik C., Lohe K., Kuang C., Xiao Y., Jouni Z, & Poels E. (2014). Modulation of adipocyte differentiation by omega-3 polyunsaturated fatty acids involves the ubiquitin-proteasome system. Journal of Cellular and Molecular Medicine, 18(4), 590-599.
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4

Immunohistochemical Analysis of Invasive Breast Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
Patient slices were obtained from the National Disease Research Interchange. All patients were diagnosed with invasive lobular/ductal carcinoma breast, Grade 2. Patients received total mastectomy or simple radical mastectomy with no known presurgical therapy. Samples were analyzed under the approved University of Texas Southwestern Medical Center Institutional Review Board protocol # STU 012017-043 (Endotrophin Upregulation in Tumor Tissues). Mouse tissues were dissected and fixed in 10% formalin overnight. Paraffin processing, embedding, sectioning and hematoxylin/eosin staining and trichrome staining were performed by John Sheldon at UTSW Medical Center. Immunostaining was performed following standard protocols, with the following antibodies: Perilipin (1:1000, #20R-PP004, Fitzgerald), EGFP (1:500, ab13970, Abcam), α-SMA (1:500, eBioscience™, #14-9760-80), CD31 (1:200, Abcam, #ab28364), Mac2 (1:750, CL8942AP, CEDARLANE) and collagen hybridizing peptide (#5276, Advanced BioMatrix). Images were acquired with LSM510 confocal microscope (Zeiss) and analyzed by Image J software.
Zhu Q., Zhu Y., Hepler C., Zhang Q., Park J., Gliniak C., Henry G.H., Crewe C., Bu D., Zhang Z., Zhao S., Morley T., Li N., Kim D.S., Strand D., Deng Y., Robino J.J., Varlamov O., Gordillo R., Kolonin M.G., Kusminski C.M., Gupta R.K, & Scherer P.E. (2022). Adipocyte mesenchymal transition contributes to mammary tumor progression. Cell reports, 40(11), 111362.
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