Anti puromycin

Manufactured by Cell Signaling Technology

Anti-puromycin is a monoclonal antibody that specifically recognizes puromycin, a translation inhibitor commonly used in cell culture experiments. This product can be used to detect and visualize newly synthesized proteins in live cells.

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Lab products found in correlation

2 protocols using anti puromycin

Protein Expression Profile Analysis

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Anti-puromycin (Kerafast EQ0001), pAKT S473 (Cell Signaling Technology (CST) 4060), p 4EBP1 T37/46 (CST 2855), Cyclin D1 (CST 55506), Estrogen Receptor Alpha N-terminus (CST 13258), Estrogen Receptor Alpha C-terminus (CST 8644), Progesterone Receptor (CST 8757), EIF4G (CST 2498), EIF4E (CST 9742), 4EBP1 (CST 9644), Beta Actin (CST 4967), Myc (CST 18583), GATA3 (CST 5852), Cyclin D3 (CST 2936), CDK4 (CST 12790), pRb S780 (CST 9307), E2F1 (CST 3742), GREB1 (CST 65171), TFF1/pS2 (CST 15571), IGFBP4 (CST 31025), cleaved PARP (CST 5625). Secondary Goat anti-Rabbit IgG (H+L) Secondary Antibody HRP (Thermo Fisher 65–6120).

Boyer J.A., Sharma M., Dorso M.A., Mai N., Amor C., Reiter J.M., Kannan R., Gadal S., Xu J., Miele M., Li Z., Chen X., Chang Q., Pareja F., Worland S., Warner D., Sperry S., Chiang G.G., Thompson P.A., Yang G., Ouerfelli O., de Stanchina E., Wendel H.G., Rosen E.Y., Chandarlapaty S, & Rosen N. (2024). eIF4A controls translation of estrogen receptor alpha and is a therapeutic target in advanced breast cancer. bioRxiv.

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Comprehensive Protein Regulation Analysis

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Cycloheximide was from Santa Cruz Biotechnology. Actinomycin-D was from Sigma. MG132 was from Selleck Chemical. Puromycin was from MP Biomedicals. Kainic acid and Thapsigargin were from Alomone Lab. Pifithrin-α was from Adipogen Corporation. Salubrinal was from Enzo Life Sciences. Dimethyl sulfoxide (DMSO) was from Fisher Scientific. DMSO was used as a vehicle in this study. The antibodies used in this study were purchased from Santa Cruz Biotechnology (anti-Mdm2, anti-Lamin A/C, anti-HSP-90), GenScript Corporation (anti-Gapdh), Millipore (anti-Puromycin), and Cell Signaling (anti-ubiquitin, anti-p-Mdm2, anti-p53, anti-BiP, anti-PDI, anti-PERK, anti-ATF6, anti-XBP1s, anti-eIF2α and anti-p-eIF2α). HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology and Cell Signaling. The lentiviral control non-target shRNA and p53 shRNA were from Santa Cruz Biotechnology.

Liu D.C., Eagleman D.E, & Tsai N.P. (2019). Novel roles of ER stress in repressing neural activity and seizures through Mdm2- and p53-dependent protein translation. PLoS Genetics, 15(9), e1008364.

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