Primaquine and erythromycin were obtained from FUJIFILM Wako Pure Chemical Corporation (Tokyo, Japan). Aflatoxin B1 and dimethyl fumarate were obtained from Sigma–Aldrich (St. Louis, MO, USA). Terbinafine was obtained from Tokyo Chemical Industry (Tokyo, Japan). These compounds were dissolved in dimethyl sulfoxide (DMSO, Sigma–Aldrich). The final concentration of DMSO in culture medium was less than 0.1%. SDS and D-mannitol were purchased from Nacalai Tesque (Kyoto, Japan) and dissolved in distilled water.
D mannitol
Manufactured by Nacalai Tesque
Sourced in Japan
D-mannitol is a sugar alcohol that is commonly used in laboratory settings. It is a white, crystalline solid with a sweet taste. D-mannitol is commonly used as a component in various laboratory procedures and experiments.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium dodecyl sulfate (sds), by Nacalai Tesque (1 mentions)
Primaquine, by Fujifilm (1 mentions)
Dimethyl fumarate, by Merck Group (1 mentions)
Aflatoxin b1, by Merck Group (1 mentions)
Erythromycin, by Fujifilm (1 mentions)
Phlorizin, by Cayman Chemical (1 mentions)
Dapagliflozin, by Cayman Chemical (1 mentions)
D glucose, by Nacalai Tesque (1 mentions)
3 protocols using d mannitol
1
Compound Preparation for Cell Assays
2
Formulation Development of Solid Dosage
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PB (as sodium salt) was purchased from LKT Laboratories Inc. (St. Paul, MN, USA), while αCD, βCD, and D-Mannitol were obtained from Nacalai Tesque Inc. (Kyoto, Japan). Microcrystalline cellulose (PH 101) was obtained from Asahi Kasei Chemicals Corp. (Tokyo, Japan), whereas Crospovidone (Kollidon CL-SF) was obtained from BASF Aktiengesellschaft (Ludwigshafen, Germany). Citric acid and Magnesium stearate were sourced from Wako Pure Ind. Co. (Osaka, Japan). All other materials and chemicals were obtained from commercial sources and were of the highest pharmaceutical or analytical grade.
3
Cardiac Fibroblast Cell Cultivation
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Materials. The CS4Z055R (containing serum) and CS4Z3500R (not containing serum) media were purchased from Cell Systems Corporation, (Kirkland, WA, USA). D-(+)-Glucose, D-(+)-mannitol, and diamidino-2-phenylindole (DAPI) were purchased from Nacalai Tesque Inc., (Kyoto, Japan). Phlorizin and dapagliflozin were purchased from Cayman Chemical Company, (Ann Arbor, MI, USA). All other chemicals were of reagent grade and commercially available.
Cell culture. Primary human cardiac fibroblast cells (ACBRI 5118) at passage 2 were purchased from Cell Systems Corporation. All studies were performed with HCFs at passage 4-10. Passage Reagent Group (Cell Systems Corporation) were used for cell passaging. The cells were seeded on 6-well tissue culture plates at a density of 1x10 5 cells/well, maintained in the CS4Z055R medium, and grown in the cell incubator at 37˚C, containing 95% O 2 and 5% CO 2 . After sub-confluence, CS4Z3500R (without serum) medium was used to synchronize the cells for 24 h. The cells were washed twice with phosphate-buffered saline and cultured in the medium to be further subjected to different treatments. Cells were passaged in 0.05% trypsin-EDTA.
Cell culture. Primary human cardiac fibroblast cells (ACBRI 5118) at passage 2 were purchased from Cell Systems Corporation. All studies were performed with HCFs at passage 4-10. Passage Reagent Group (Cell Systems Corporation) were used for cell passaging. The cells were seeded on 6-well tissue culture plates at a density of 1x10 5 cells/well, maintained in the CS4Z055R medium, and grown in the cell incubator at 37˚C, containing 95% O 2 and 5% CO 2 . After sub-confluence, CS4Z3500R (without serum) medium was used to synchronize the cells for 24 h. The cells were washed twice with phosphate-buffered saline and cultured in the medium to be further subjected to different treatments. Cells were passaged in 0.05% trypsin-EDTA.
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