The sample of 0.2% 11S was made with the 0.01 mM pH 7.0 phosphate buffer solution. The structural changes of 11S with MEL-A at different concentrations (0–1.0 mg/mL) and 11S by the addition of 0.5 mg/mL MEL-A after heat treatment of 0–9.0 h. CD spectroscopy was performed at 25 °C using an instrument J-1500–150ST (JASCO, Tokyo, Japan) from 195 nm to 300 nm. Each measurement was repeated three times. The structural parameters were analyzed by Dichroweb.
J 1500 150st
Manufactured by Jasco
Sourced in Japan
The J-1500-150ST is a laboratory equipment product designed for general use in research and testing environments. It provides a core function of measuring and analyzing scientific data. The specific details and intended applications of this product are not available for an unbiased and factual description.
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Lab products found in correlation
4 protocols using j 1500 150st
1
Structural Changes of 11S by MEL-A
2
Silk Protein Secondary Structure Analysis
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CD spectroscopy
was used to study the secondary structure of the silk proteins. Silk
films were prepared on the side of a quartz crystal cuvette using
50 μL of 0.5 mg/mL silk protein. CD spectra between 180 and
260 nm were measured with a Jasco J-1500-150ST instrument (1 nm bandwidth
and average of eight accumulations).
was used to study the secondary structure of the silk proteins. Silk
films were prepared on the side of a quartz crystal cuvette using
50 μL of 0.5 mg/mL silk protein. CD spectra between 180 and
260 nm were measured with a Jasco J-1500-150ST instrument (1 nm bandwidth
and average of eight accumulations).
3
CD Spectrometry of Biomolecules
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A J-1500-150ST (Jasco) CD Spectrometer with a peltier temperature control system was used to collect CD spectra. All measurements were collected at 25 °C between 190 and 260 nm with a pitch of 1 nm at a scan speed of 50 nm min–1, a response time of 4 s, slit widths of 2 nm, and standard sensitivity.
4
CD Spectroscopy of Enzyme Solutions
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Enzymes with or without sonication treatment (optimal ultrasonic conditions) were dissolved in 10 mmol/L phosphate buffer (0.3 mg/mL, pH 7.0). The CD (JASCO, J-1500-150ST) was captured in the wavelength range 190–260 nm, with a bandwidth of 1 nm and a scan speed of 100 nm/min, using a 1.0 mm quartz cell. Each spectrum was the average of three consecutive scans and background signal subtracted (phosphate buffer (10 mmol/L, pH 7.0)). The raw data was analyzed on the DichroWeb (http://dichroweb.cryst.bbk.ac.uk/ ) [31] (link).
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