Cgp42112a

Manufactured by Merck Group

Sourced in United States

CGP42112A is a laboratory equipment product. It is a chemical compound used in various research and scientific applications.

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Lab products found in correlation

7 protocols using cgp42112a

Investigating HPMC Responses to HG and CGP-42112A

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HPMC (HmrSV5) was purchased from Shanghai LMAI Biotech (L547). The cells were cultured in low glucose Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco,10567-014) containing 10% fetal bovine serum (Gibco, 16000044). HPMCs were starved in serum-free DMEM containing 0.2% bovine serum albumin (BSA) (Sigma, Poole, Dorset, UK) for 24 h before the experiment. And the cells subsequently stimulated with HG, or CGP-42112A (Sigma, 127060-75-7).

Liu J., Jin B., Lu J., Feng Y., Li N., Wan C., Zhang Q.Y, & Jiang C.M. (2022). Angiotensin II type 2 receptor prevents extracellular matrix accumulation in human peritoneal mesothelial cell by ameliorating lipid disorder via LOX‐1 suppression. Renal Failure, 44(1), 1687-1697.

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Probing AT2R Signaling Pathways

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TGF-β1 and CGP42112A were obtained from Sigma (St. Louis, MO, USA). The AT₂R antagonists, PD123319 and PD123177, and cycloheximide were also obtained from Sigma. Antibodies against TGF-βRII and E-cadherin were rabbit anti-human polyclonal antibodies. The antibody against α-SMA was mouse monoclonal antibody. The antibody against AT₂R was an affinity purified goat polyclonal antibody. All of the antibodies were obtained from Santa Cruz Biotechnology, Inc (Dallas, TX, USA). All other chemicals for various buffers were of the highest purity available and purchased exclusively from Sigma or Gibco (Gibco, Grand Island, NY, USA).

Guo H.L., Liao X.H., Liu Q, & Zhang L. (2016). Angiotensin II Type 2 Receptor Decreases Transforming Growth Factor-β Type II Receptor Expression and Function in Human Renal Proximal Tubule Cells. PLoS ONE, 11(2), e0148696.

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NCC Phosphorylation in Hypertension

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Angiotensin II, hydrochlorothiazide (HCTZ), CGP42112A, losartan and PD123319 were purchased from Sigma (St Louis, MO). The NCC antibody was a gift from Robert Hoover at Emory University, whereas antibody of phosphorylated NCC at threonine 53 (Thr⁵³) was kindly provided by David H. Ellison at Oregon Health & Science University. The data are shown as mean ± SEM. We used the paired t test or one-way ANOVA test to determine the statistical significance.

Wu P., Gao Z.X., Duan X.P., Su X.T., Wang M.X., Lin D.H., Gu R, & Wang W.H. (2018). AT2R-mediated regulation of Na-Cl cotransporter (NCC) and renal K excretion depends on the K channel, Kir4.1. Hypertension (Dallas, Tex. : 1979), 71(4), 622-630.

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Signaling Pathway Modulation Assays

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Lipopolysaccharide (LPS), interferon-γ (IFN-γ), Ang II, CGP-42112A, Ang [1–7], candesartan, PD123319, and A779 were purchased from Sigma-Aldrich (St. Louis, MO, USA). Forskolin was purchased from Tocris (Bristol, UK). The concentrations of ligands used for signaling assays were selected on the basis of the dose–response experiments shown in Supplementary Figure S1.

Rivas-Santisteban R., Lillo J., Muñoz A., Rodríguez-Pérez A.I., Labandeira-García J.L., Navarro G, & Franco R. (2021). Novel Interactions Involving the Mas Receptor Show Potential of the Renin–Angiotensin system in the Regulation of Microglia Activation: Altered Expression in Parkinsonism and Dyskinesia. Neurotherapeutics, 18(2), 998-1016.

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Isolation and Culturing of Adult Neural Stem Cells

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NSCs were isolated from the V-SVZ of the lateral ventricle of adult young (3–4 months old), aged (20 months old) male mice, or young AT1-KO and AT2-KO mice as previously described [49 (link),50 (link)]. Primary neurospheres were counted after 7 days in vitro. For passages, neurospheres were mechanically dissociated to a single-cell suspension and reseeded in growth media supplemented with the specific treatments: Ang II (100 nM; Sigma-Aldrich); ZD7155 or candesartan (AT1 receptor blockers; 1 μM; Tocris, Bristol, United Kingdom); PD123319 (AT2 receptor blocker; 1 μM; Sigma-Aldrich); CGP42112A (Sigma-Aldrich) or C21 (AT2 receptor agonists; 1 μM); Ang1-7 (1 μM; Sigma-Aldrich); and A779 (MasR blocker; 1 μM; Bachem, Bubendorf, Switzerland). The doses were selected according to suggestions from previous studies [33 (link),35 (link),51 (link),52 (link),53 (link),54 (link),55 (link)]. The diameters of primary and secondary neurospheres were measured after 7 or 5 days, respectively.

Garcia-Garrote M., Perez-Villalba A., Garrido-Gil P., Belenguer G., Parga J.A., Perez-Sanchez F., Labandeira-Garcia J.L., Fariñas I, & Rodriguez-Pallares J. (2019). Interaction between Angiotensin Type 1, Type 2, and Mas Receptors to Regulate Adult Neurogenesis in the Brain Ventricular–Subventricular Zone. Cells, 8(12), 1551.

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Stimulating Immune Responses in Cells

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Lipopolysaccharide (LPS), interferon-γ (IFN-γ), and forskolin, Angiotensin II (Ang II), CGP-42112A (CGP), Candesartan (CAN) and PD123319 (PD) were purchased from Sigma-Aldrich (St Louis, MO).

Rivas-Santisteban R., Rodriguez-Perez A.I., Muñoz A., Reyes-Resina I., Labandeira-García J.L., Navarro G, & Franco R. (2020). Angiotensin AT1 and AT2 receptor heteromer expression in the hemilesioned rat model of Parkinson’s disease that increases with levodopa-induced dyskinesia. Journal of Neuroinflammation, 17, 243.

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Cardiovascular Pharmacological Interventions

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Captopril, Olmesartan, CGP42112A, and alloxan monohydrate were purchased from Sigma Chemical Co. (Saint Louis, MO, USA). PD123319 from Cayman Chemical Co. (Ann Arbor, MI, USA). Ethanol, methanol, and xylene from Merck (Rio de Janeiro, RJ, Brazil). Sodium heparin and sterile saline solution from Roche (São Paulo, SP, Brazil). All solutions were prepared immediately before use.

Chaves A.D., Magalhães N.S., Insuela D.B., Silva P.M., Martins M.A, & Carvalho V.F. (2022). Effect of the renin-angiotensin system on the exacerbation of adrenal glucocorticoid steroidogenesis in diabetic mice: Role of angiotensin-II type 2 receptor. Frontiers in Endocrinology, 13, 1040040.

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