Sw620

Manufactured by Lonza

Sourced in Switzerland, United Kingdom, France

The SW620 is a laboratory instrument designed for cell culture applications. It serves as an incubator, maintaining optimal temperature, humidity, and atmospheric conditions for cell growth and proliferation.

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Lab products found in correlation

Sw620, by American Type Culture Collection (5 mentions) Ht 29, by Lonza (4 mentions) Sw480, by Lonza (3 mentions) Sw480, by American Type Culture Collection (3 mentions) Caco 2, by Lonza (2 mentions) Caco 2, by American Type Culture Collection (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin, by Lonza (1 mentions) Streptomycin, by Lonza (1 mentions) Foetal bovine serum (fbs), by Lonza (1 mentions) Turbogfp, by Merck Group (1 mentions) Mcf 7, by Merck Group (1 mentions) Mda mb 231, by Merck Group (1 mentions) Hcc1954, by American Type Culture Collection (1 mentions) Sw480, by Thermo Fisher Scientific (1 mentions) Hec1a, by Lonza (1 mentions) Hcc1937, by Lonza (1 mentions) Hcc1954, by Lonza (1 mentions) Penicillin streptavidin, by Thermo Fisher Scientific (1 mentions)

6 protocols using sw620

Glucose Dosage Effects on Colon Cancer Cells

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The American Type Culture Collection provided the colon cancer cell line Caco2, SW620, and normal colon cells FHC (ATCC, Manassas, VA, USA). The cells were grown in DMEM media with low glucose content (1.0 g/L, 5.6 mmol/L). In addition, 10 percent foetal bovine serum (FBS) (Lonza Bioproducts), penicillin 100 U/mL, and streptomycin 100 mg/mL (Lonza Bioproducts) were added to the media, which was incubated at 37 °C in a humidified chamber with 5% CO 2 .
Caco2, SW620, and FHC colon cancer cell lines were planted in 6-well plates as indicated above. All cell lines were cultured for 24 hours (3 wells per treatment) with different doses of D-(+)-glucose [5 Mm (90 mg/dL), 10 mM (181 mg/dL), and 15 mM (271 mg/dL)].

Mosaad H., Shalaby S.M., Mahmoud N.M., Ahmed M.M., Fayed A., Ashour H.R, & Sarhan W. (2024). LncRNA ANRIL Promotes Glucose Metabolism and Proliferation of Colon Cancer in a High-Glucose Environment and is Associated with Worse Outcome in Diabetic Colon Cancer Patients. Asian Pacific journal of cancer prevention : APJCP, 25(4).

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Establishing Diverse Cell Line Panel

Cited 1 time

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The following tumour cell lines were purchased from ECACC directly from Sigma-Aldrich:breast: MDA-MB-231[16 (link)](92020424), MCF7[17 (link)] (86012803); colon: HT29[18 (link)] (91072201), SW480[18 (link)] (87092801), SW620[18 (link)] (87051203); lung: A549[19 (link)] (86012804) and prostate: PC-3[20 (link)] (90112714); as well as endothelial cells: HUVEC (S200-05N) and leucocytes: Jurkat cells[21 (link)] (88042803).The brest cancer tumour cell HCC1937 lines[22 (link)](ATCC-CCL-247)and HCC1954[23 (link)] (ATCC CRL-2338) were purchased from ATCC; the endometrial cell lines HEC1A and HEC1A stably expressing the ETV5 transcription factor (HEC1A-ETV5) were previously described [24 (link)–26 (link)]. Medium for cell culture were obtained from Gibco (DMEM: SW480, SW620, A549, MCF7, MDA-MB-231, PC-3; McCoyy᾿s: HT29, HEC1A, HEC1A-ETV5; RPMI 1640: HCC1937, HCC1954,Jurkat), and from Lonza (Basel, Switzerland) (EGM-2: Huvec). The medium was supplemented with 10% FBS and 1% streptavidin-penicillin (Life Technologies, Carlsbad Ca, USA). Cells were maintained at 37°C in a 5% CO₂ incubator. For selected experiments, we transduced MDA-MB-231 and SW480 cells with lentiviral transduction particles (turboGFP, Sigma-Aldrich) to establish cells that expressed green fluorescent protein (GFP); cells were further selected with puromycin (5 μg/mL) and checked by flow cytometry (96%).

Vila A., Abal M., Muinelo-Romay L., Rodriguez-Abreu C., Rivas J., López-López R, & Costa C. (2016). EGFR-Based Immunoisolation as a Recovery Target for Low-EpCAM CTC Subpopulation. PLoS ONE, 11(10), e0163705.

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Culturing Human Colon Cancer Cell Lines

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Eight human colon cancer cell lines, SW1116, SW480, DLD-1, SW620, HT-29, Caco-2, COLO205 and T84 were obtained from the American Type Culture Collection (ATCC). SW1116, SW480, DLD-1, SW620, HT-29 and Caco-2 were cultured in DMEM media (Lonza) supplemented with 10% fetal bovine serum (FBS) (Sigma-Aldrich), 1% L-glutamine (Sigma-Aldrich) and 1% penicillin/streptomycin (Sigma-Aldrich). COLO205 was maintained in RPMI 1640 media (Lonza) supplemented with 10% fetal bovine serum (FBS), 1% L-glutamine and 1% penicillin/streptomycin. T84 was maintained in DMEM/Ham's F12 media (Lonza) supplemented with 10% FBS, 1% L-glutamine and 1% penicillin/streptomycin. Cells were grown at 37°C in a humidified atmosphere of 5% CO₂.
Dukes' stage and tissue origin for each cell line is shown in Table S1.

Vázquez-Iglesias L., Barcia-Castro L., Rodríguez-Quiroga M., Páez de la Cadena M., Rodríguez-Berrocal J, & Cordero O.J. (2019). Surface expression marker profile in colon cancer cell lines and sphere-derived cells suggests complexity in CD26+ cancer stem cells subsets. Biology Open, 8(7), bio041673.

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Authenticated Colorectal Cancer Cell Lines Protocol

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Authenticated CRC lines were obtained from ECACC: HT29 (ECACC 91072201), Colo205 (ECACC 87061208), Colo320DM (ECACC 87061205), SW480 (ECACC 87092801), and SW620 (ECACC 87051203). Authenticated primary human colonocytes were obtained from ATCC: CCD-18Co (ATCC CRL-1459) and CCD-841 CoN (ATCC CRL-1790). All lines were regularly monitored for mycoplasma contamination every 2 months by PCR^{40 (link)}, and only mycoplasma-free cells were used for studies. All cells were used for no more than 15 passages. All cells were grown at 37 °C in HEPA-filtered humidified air in 5% CO₂, in media supplemented with 10% heat-inactivated FBS (Gibco, Paisley, Scotland, UK, Cat. no. 10099-141), L-Glu (2 mM, Lonza, Burton on Trent, UK, Cat. no. 17-606E) and penicillin/streptomycin (100 U/ml, Gibco, Cat. no. DE17-602E). Colo205 and Colo320DM cells were grown in RPMI medium (Gibco, Paisley, Scotland, UK, Cat. no. 31870), HT29 were grown in McCoy’s medium (Lonza, Burton on Trent, UK, Cat. no. 12-688F), SW480 and SW620 were grown in L15 medium (Lonza, Burton on Trent, UK, Cat. no. BE12-700F), and HCT-116 (17) were grown in DMEM (Gibco, Paisley, Scotland, UK, Cat. no. 21885-025). CCD-18Co and CCD-841 primary cultures were grown in EMEM (Sigma-Aldrich, Saint Louis, MO, USA, Cat. no. M2279).

Stramucci L., Pranteda A., Stravato A., Amoreo C.A., Pennetti A., Diodoro M.G., Bartolazzi A., Milella M, & Bossi G. (2019). MKK3 sustains cell proliferation and survival through p38DELTA MAPK activation in colorectal cancer. Cell Death & Disease, 10(11), 842.

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Characterization of Cancer Cell Lines

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U2OS, PANC-1, NUGC-3, SW620, and MIA PaCa-2 were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA). The BJ-hTER and BJ-RasV12 cell were provided by W. Hahn (Dana-Farber Cancer Institute) and the DR-GFP U2OS cells were provided by M. Jasin (Memorial Sloan Kettering Cancer Center). U2OS, DR-GFP U2OS, BJ-hTERT, and BJ-RasV12 cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% fetal bovine serum (FBS, Life Technologies/ThermoFisher Scientific), and penicillin–streptomycin antibiotics (50 U/mL). MIA PaCa-2, PANC1, SW620, and SW480 were grown in DMEM (Lonza Biosciences), with 10% FBS (Life Technologies/ThermoFisher Scientific). Cells were grown at 37 °C in 5% CO₂ humidified incubators.

Gustafsson N.M., Färnegårdh K., Bonagas N., Ninou A.H., Groth P., Wiita E., Jönsson M., Hallberg K., Lehto J., Pennisi R., Martinsson J., Norström C., Hollers J., Schultz J., Andersson M., Markova N., Marttila P., Kim B., Norin M., Olin T, & Helleday T. (2018). Targeting PFKFB3 radiosensitizes cancer cells and suppresses homologous recombination. Nature Communications, 9, 3872.

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Culturing Colorectal Cancer Cell Lines

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The human colorectal carcinoma HCT116, colorectal adenocarcinoma HT29, HCT8, SW480, SW620, LoVo and SW48 cell lines were obtained from the American Type Culture Collection (ATCC). Some cell lines were grown in RPMI 1640 with ultraglutamine (Lonza, Levallois, France) (HCT116, HT29, HCT8, SW480) or in DMEM 4.5g/L glucose (Lonza) (SW620, LoVo, SW48) supplemented with 10% (vol/vol) fetal bovine serum (FBS; Lonza). The human normal mucosa cell line NCM460 was obtained from INCELL corporation LLC (San Antonio, TX) and was grown in M3Base medium (INCELL corporation LLC) supplemented with 10% FBS. All cell lines were grown in an atmosphere of 95% air and 5% CO₂ at 37°C.

Courtaut F., Derangère V., Chevriaux A., Ladoire S., Cotte A.K., Arnould L., Boidot R., Rialland M., Ghiringhelli F, & Rébé C. (2015). Liver X Receptor ligand cytotoxicity in colon cancer cells and not in normal colon epithelial cells depends on LXRβ subcellular localization. Oncotarget, 6(29), 26651-26662.

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