Invia raman microscope

Raman spectra of Np containing solid samples and solutions were collected on a Renishaw inVia Raman Microscope with a circularly polarized excitation line of 532 nm. Due to the radiological hazards associated with ²³⁷Np, each solid sample or 2 μL solution sample was placed on a glass drop slide covered with a transparent coverslip, which was sealed to the slide using epoxy. Numerous spectra from multiple spots were collected on multiple samples for each compound and on each solution to ensure sample homogeneity. All Np solutions used for Raman spectral analysis were prepared individually by mixing an aliquot of the 0.24 M Np^V stock solution with appropriate volumes of ACl stock solutions and H₂O.

To evaluate the molecular structure for the untreated and treated samples, Raman spectroscopy was performed using a commercial instrument (inVia Raman microscope, Renishaw, Wotton-under-Edge, UK). Raman spectra were generated using a 784 nm laser source with 10 s exposure on the surface of the sample with a 50× objective. The spectra were obtained over wavelengths from 100 to 3200 cm⁻¹. Samples of material for Raman spectroscopy were sectioned from the grip sections of the fractured samples. Baseline correction, normalization and smoothing of the Raman spectra were achieved by data analysis software (Origin, OriginLab, Northampton, MA, USA). In addition, WiRE software (Version 5.2) was used to acquire and compare the Raman spectra for Ultem 1000 to the spectra for PC.

Raman microscopy was conducted on inorganic CND/host nanocomposites grown on glass substrates using a Renishaw inVia Raman Microscope (785 nm laser) with a 50× objective using MS20 encoded sample stage control through rollerball XYZ peripheral. Data acquisition was undertaken with Renishaw WiRE 3.4 with a laser intensity of 0.1% under three accumulated acquisitions (3 × scan time 30 s) between 1200 and 100 cm⁻¹.
Raman spectroscopy of CNDs was conducted using a Renishaw inVia Raman confocal inverted microscope integrated with a Leica DMi8/SP8 laser scanning confocal microscope system, with a 785 nm diode laser (laser power of 4.5 mW on the sample, intensity of ~ 5.7 × 105 W cm⁻²) and a 1200 l mm⁻¹ grating. Light was collected using a near infrared enhanced CCD array detector (1024 × 256 pixels). Prior to every experiment, a spectrum of a silicon sample was collected and the microscope was calibrated to the peak position (520.5 cm⁻¹). The sample was drop cast onto a quartz slide from an aqueous suspension and dried under nitrogen. Spectra were collected with a 40× objective (NA 0.85 HCX PL APO CORR CS) acquiring for 200 s. Baseline subtraction was done using the Matlab function f_baseline_corr with bandwidth of 350, smoothwidth of 30 and 20 iterations^{50 (link)}. Spectra were analysed with reference to Raman spectra of folic acid^{51 (link)} and riboflavin^{52 (link)}.