Anti aib1

Equal amounts of protein lysates were separated by SDS-PAGE and transferred onto PVDF membranes. Filters were probed with the following specific primary antibodies: anti-GCN5 (Santa-cruz), anti-AIB1 (BD Bioscience) anti-p21^Cip1/Waf1 (BD Biosciences), PCNA (Abcam, Cambridge, MA, USA), β-actin (Sigma, St Louis, MO, USA), Akt and phosphorylated-Akt (Cell signaling, Danvers, MA, USA), Anti-histone H3 (acetyl K9) antibody-ChIP Grade (Abcam, Cambridge, MA, USA) anti-CyclinD1 (Abcam, Cambridge, MA, USA). Blots were then incubated with horseradish peroxidase-conjugated secondary antibody (Pierce, Rockford, IL, USA) and visualized by chemiluminescence. The band density was quantified by densitometry using Scion Image software and normalized to β-actin levels.