At 48 h after the last allergen skin sensitization, the pups were weighed and baseline rectal temperatures of the pups were taken using a BAT-12 Microprobe Thermometer with a RET-4 thermocouple sensor type T rectal probe for neonatal mice (Physitemp Instruments Inc). Then, the pups received PNE by gavage (100 μl of 10 mg protein/ml sterile water) using a 24 gauge gavage needle (Pet Surgical, Agoura Hills, CA). Rectal temperatures were taken every 15–20 min for up to 80 min. The pups were placed back with the mothers. Then 4 h later, the pups were euthanized and tissues collected.
Bat 12 microprobe thermometer
Manufactured by Physitemp
Sourced in United States
The BAT-12 Microprobe Thermometer is a laboratory instrument designed to measure temperature. It features a digital display and can be used with various temperature probes.
Automatically generated - may contain errors
Lab products found in correlation
Ret 3 rectal probe, by Physitemp (3 mentions)
Mds032280, by Physitemp (3 mentions)
Ret 3 rectal probe for mice, by Physitemp (3 mentions)
Flir c2, by Teledyne (2 mentions)
Cl 100 temperature controller, by Harvard Apparatus (2 mentions)
Cl316 243, by Merck Group (2 mentions)
Fura 2 am, by Thermo Fisher Scientific (2 mentions)
Tix660 infrared camera, by Fluke (1 mentions)
Pclamp 9, by Molecular Devices (1 mentions)
Tse labmaster, by TSE Systems (1 mentions)
Activity monitoring chambers, by Harvard Apparatus (1 mentions)
Shm 828, by Harvard Apparatus (1 mentions)
It 23, by Physitemp (1 mentions)
Ret 3 probe, by Physitemp (1 mentions)
Flir i3, by Teledyne (1 mentions)
D12492, by Research Diets (1 mentions)
E60bx compact infrared thermal imaging camera, by Teledyne (1 mentions)
Clams hc, by Columbus Instruments (1 mentions)
Flir t650sc, by Teledyne (1 mentions)
Clampex software, by Molecular Devices (1 mentions)
47 protocols using bat 12 microprobe thermometer
1
Allergen-Induced Thermogenic Response
2
Thermoregulation in UCP1 Knockout Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were approved by the North Stockholm Animal Ethics Committee. UCP1 KO mice originated from the institute's own breeding and were the descendants of those earlier described [18] ; they had been back-crossed to wildtype C57Bl/6 for at least ten generations; the wildtype (C57Bl/6) mice were obtained from Nova, Germany. During experimentation, mice were housed at 30 °C with relative humidity of 45–52%, and a 12 h/12 h light–dark cycle. They were single caged and had free access to standard chow diet and water. Body weight and food consumption were measured daily. Food consumption was determined by weighing the food offered and the remaining food in the cages every day. Rectal temperature was measured using a rectal probe for mice and a BAT-12 microprobe thermometer (Physitemp Instruments, Inc. USA). These procedures were performed daily at 30 °C ambient temperature between 10 a.m. and 2 p.m.
3
Body Temperature Monitoring in Mice
4
Rectal Temperature Measurement in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Measurement of rectal temperature in mice was performed in the breeding room to avoid possible additional stressful factors. Mice were arbitrarily picked up without identification and the probe of a BAT-12 Microprobe Thermometer (Physitemp Instruments, Clifton, NJ, USA), which was immersed in mineral oil before use, was placed approximately 10 mm into the rectal canal after lifting the tail to measure the rectal temperature. During the procedure, the mice were placed on a metal grid. Mouse identification was performed after the measurement of each subject. After the measurement, the mice were placed in a holding cage until all the mice from the same home cage had completed the test.
5
Daily Metabolic Monitoring in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Every day (from day 0 to day 6) between 10:30–11:00 AM, mice were taken out of the chambers (if relevant), and body temperature was determined with a rectal probe for mice (BAT-12 microprobe thermometer, Physitemp Instruments, Inc; Clifton, NJ, USA). Body weight and the quantity of food consumed were also measured at this time. Food was weighed when supplied, and remaining food was subtracted to calculate the food eaten per day.
6
Selective Brain Hypothermia Induction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Selective cerebral hypothermia was performed according to a previously published protocol (Kurisu et al., 2016a), Briefly, 4°C (cold) saline was infused (20 mL/kg) through a microcatheter placed in the right internal carotid artery via the external carotid artery for 15 minutes immediately after removal of the filament in the hypothermia group. To control for the effect of hemodilution by the infused saline, 37°C (warm) saline was infused in the same manner in the normothermia group. To ensure that selective brain hypothermia was successfully produced, cortical and rectal temperatures were monitored during the saline infusion. One rat died from hypothermia, and was replaced in subsequent experiments. Needle thermistor probes (BAT-12 Microprobe Thermometer; Physitemp Instruments, Inc., Clifton, NJ, USA) were placed into the cortex through holes 3 mm lateral to the bregma, 3 mm posterior to the bregma, and 3 mm lateral to the bregma on the ipsilateral side to monitor cortical temperature. Body temperatures were measured through the rectum. The rats were returned to their cages with free access to food and water and were closely monitored.
7
Cold Exposure Thermal Monitoring
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The mice were subjected to a cold room (4 °C) without access to food or water. The rectal temperature was measured at the indicated time after exposure to the cold, using a rectal probe connected to digital thermometer (BAT-12 Microprobe-Thermometer; Physitemp, USA). Skin temperature was recorded with an infrared camera (Fluke TiX660 Infrared Camera, Fluke Corporation, Washington, USA) and analyzed with a specific software package (Smartview3.14.45 Fluke Corporation, Washington, USA).
8
Acute Cold Tolerance in Mice
9
Modeling Salmonella Typhimurium Infection in Mice
10
Locomotor Activity and Analgesia Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Locomotor activity was assessed in clear Plexiglas activity chambers (47 cm × 25.5 cm × 22 cm). Each chamber was surrounded by 2 arrays of 4 × 8 photocell infrared beams, interfaced with software for automated data collection (San Diego Instruments, San Diego, CA, USA). Temperature readings were taken using a BAT-12 Microprobe Thermometer with RET-3 Rectal Probe (PhysiTemp Instruments Inc., Clifton, NJ, USA). Analgesia was measured with a Tail Flick Analgesia Meter (IITC Inc. Life Science, Woodland Hills, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!