5-(and-6)-carboxy-2′,7′-dichlorofluorescein (CDCF) and 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA) were purchased from AAT Bioquest (Sunnyvale, CA, United States). Digoxin, estrone 3-sulfate (E3S), and lopinavir were purchased from Sigma-Aldrich (St. Louis, MO, United States). Nelfinavir was obtained from APExBIO Technology (Houston, TX, United States). Darunavir was purchased from Toronto Research Chemicals (North York, ON, Canada). A rabbit monoclonal P-gp antibody (ab120904), a rabbit monoclonal MRP2 antibody (ab172630) and a rabbit monoclonal GAPDH antibody (ab181602) were purchased from Abcam (Cambridge, MA, United States), and a rabbit monoclonal BCRP antibody (CST42078) was obtained from Cell Signaling Technology (Danvers, MA, United States). All other chemicals and solvents were of the highest grade commercially available.
Ab172630
Manufactured by Abcam
Sourced in United States
Ab172630 is a laboratory reagent product manufactured by Abcam. It is a polyclonal antibody that targets a specific antigen. The core function of this product is to serve as a detection and/or analytical tool in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Darunavir, by LGC (1 mentions)
Nelfinavir, by Apexbio (1 mentions)
Digoxin, by Merck Group (1 mentions)
Estrone 3 sulfate, by Merck Group (1 mentions)
Lopinavir, by Merck Group (1 mentions)
Ab181602, by Abcam (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Ab129089, by Abcam (1 mentions)
Ab234982, by Abcam (1 mentions)
Ab131084, by Abcam (1 mentions)
Ab155421, by Abcam (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
One step rt pcr kit, by Thermo Fisher Scientific (1 mentions)
Rnase free dnase set, by Qiagen (1 mentions)
Ab137550, by Abcam (1 mentions)
Ab32087, by Abcam (1 mentions)
Ab181598, by Abcam (1 mentions)
Ab16667, by Abcam (1 mentions)
Ab32572, by Abcam (1 mentions)
Mounting medium, by Thermo Fisher Scientific (1 mentions)
6 protocols using ab172630
1
Fluorescent Probe Assay for Transporter Inhibition
2
Comprehensive Analysis of Hepatic Transporters
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HB (collection in Sichuan, 20042901), ZZ (collection in Fujian, 210303), DH (collection in Gansu, 20201211), and MX (collection in Jiangsu, 21032501) were obtained from Simcare Ltd. Primary antibodies against ZO-1 (21773-1-AP, 1:1000), occludin (13409-1-AP, 1:1000), claudin-1 (13050-1-AP, 1:1000), and the second antibody of beta-actin (20536-1-AP, 1:10000) were purchased from Proteintech Group Inc. Primary antibodies against NTCP (ab131084, 1:1000), BSEP (ab155421, 1:1000), CYP7A1 (ab234982, 1:1000), MRP2 (ab172630, 1:1000), and FXR1 (ab129089, 1:1000) were obtained from Abcam Inc. ANIT (N106389), UDCA(U110695), and olive oil (O108685) were purchased from the Aladdin Chemical Reagent Co. Dimethyl sulfoxide (DMSO) was purchased from Sigma-Aldrich (reference D4540) (St-Louis, MO, USA). The TRIzol total RNA extraction kit was obtained from Life Technologies.
3
Rosemary Extract's Antioxidant Mechanisms
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rosemary extract (RE, contained carnosic acid 23.2%, carnosol 12.4%), carnosic acid (CA, above 99% purity), and carnosol (CL, above 99% purity) were obtained from OnRoad Biotech Co. (Changsha, China). MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] was from THEMO cleaved caspase-3. ELISA kit was obtained from Cusabio Biotech Co. Ltd. (Wuhan, China). Antibodies for Western Blot: anti-Nrf2 antibody (ab137550) and anti-MRP2 antibody (ab172630) were from Abcam (USA). Sestrin2 (H-62) antibody was from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). BCA protein assay kit was obtained from Beijing ComWin Biotech Co., Ltd. (Beijing, China). One-Step RT-PCR kit was obtained from Life Technologies (Grand Island, NY, USA). RNeasy mini kit and RNase-Free DNase set for RNA extraction were obtained from QIAGEN (Santa Clarita, CA, USA).
4
Profiling Tumor Microenvironment Characteristics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Relative changes in proliferative ability, physioxia, anti-apoptosis, drug resistance and intracellular pH levels were measured.
Monolayer cells or spheroids were fixed with 4% neutral formalin and then stained with primary antibodies against Ki-67 (ab16667, 1:100, Abcam), HIF-1α (ab1, 1:100, Abcam), MCL-1 (ab32087, 1:100, Abcam), and MRP2 (ab172630, 1:100, Abcam) to determine their characteristics.
Cytosolic pH was measured using the fluorescent dye probe Protonex™ Red 600 (Cat# 21,207, 1 μM; AAT Bioquest) following the manufacturer's instructions. Monolayer cells or spheroids were incubated at 37°C for 30 min. Cells were then gently washed in Hanks solution with 20 mM HEPES and analyzed.
In addition, monolayer cells or spheroids were also stained with N-cadherin (66219-1-Ig, 1:100, Proteintech), β-catenin (ab32572, 1:250, Abcam), and cytokeratin 7 (ab181598, 1:100, Abcam) to determine cell adhesion and interaction.
Monolayer cells or spheroids were fixed with 4% neutral formalin and then stained with primary antibodies against Ki-67 (ab16667, 1:100, Abcam), HIF-1α (ab1, 1:100, Abcam), MCL-1 (ab32087, 1:100, Abcam), and MRP2 (ab172630, 1:100, Abcam) to determine their characteristics.
Cytosolic pH was measured using the fluorescent dye probe Protonex™ Red 600 (Cat# 21,207, 1 μM; AAT Bioquest) following the manufacturer's instructions. Monolayer cells or spheroids were incubated at 37°C for 30 min. Cells were then gently washed in Hanks solution with 20 mM HEPES and analyzed.
In addition, monolayer cells or spheroids were also stained with N-cadherin (66219-1-Ig, 1:100, Proteintech), β-catenin (ab32572, 1:250, Abcam), and cytokeratin 7 (ab181598, 1:100, Abcam) to determine cell adhesion and interaction.
5
Immunofluorescence Microscopy of MRP2 and F-actin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunofluorescence analysis was performed as described previously [9 (link)]. The cells were incubated with a primary antibody directed against rabbit anti-MRP2 (ab172630; Abcam) and mouse anti-flag (#8146; Cell Signaling technology) at 4 °C overnight. After three washes with phosphate-buffered saline for 5 min each, the cells were incubated with anti-rabbit Alex 647 and anti-mouse Alex 488-conjugated secondary antibodies (1:200; Invitrogen) for 1 h at room temperature. For F-actin staining, cells were incubated with FITC-conjugated phalloidin at 50 μg/ml (P5282; Sigma) for 40 min. Then cells were mounted on a slide in mounting medium (Molecular Probes). Finally, the cells were visualized and photographed using an FV 300 confocal microscope (Olympus, Tokyo, Japan).
6
Western Blot Analysis of Cellular Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein was extracted from the cells using RIPA lysis buffer (CWBIO, China), followed by subjected to SDS-polyacrylamide gels and transferred to polyvinylidene difluoride membranes. The membranes were blocked with 5% BSA for 1 hour at room temperature. Corresponding primary antibodies including anti-LIF (1:500, ab138002, Abcam), anti-STAT3 (1:1000, 9139, CST), anti-p-STAS3(Tyr705) (1:1000, 9145, CST), anti-CAV1 (1:1000, ab32577, Abcam), anti-SOX2 (1:1000, ab92494, Abcam), anti-ABCC2 (1:1000, ab172630, Abcam), anti-CDA (1:1000, ab222515, Abcam), anti-ZNRF1 (1:1000, ab175125, Abcam), anti-ubiquitin (1:1000, 3936, CST), anti-GAPDH (1:1,000, abs132004, Absin) were added to the membrane at 4℃ overnight. HRP-conjugated secondary antibodies were used. The protein bands were visualized by ECL detection system (Millipore, Germany).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!