The visual acuity of the mice was determined by use of optomotor response recordings using OptoMotry 1.7.7 (Cerebral Mechanics, Canada) [44 (link), 45 (link)].
Optomotry 1
Manufactured by Cerebral Mechanics
Sourced in Canada
The OptoMotry 1.7.7 is a laboratory equipment used for visual function assessment. It is a high-resolution virtual reality system that generates virtual environments for the measurement of optokinetic responses in rodents and other small animals.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using optomotry 1
1
Optomotor Response for Visual Acuity
2
Quantitative Assessment of Visual Acuity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To obtain an objective and quantitative assessment of visual function capacity, we employed the optokinetic reflex (OKR) method, a computer-based means of assessing visual [52 (link)] (OptoMotry© 1.7.7; Cerebral Mechanics). Experiments were carried out as previously described in Gil et al. [53 (link)]. Spatial frequency was increased gradually, and visual acuity was determined when the mouse no longer reacted to the rotating gratings.
3
Evaluating Visual Acuity in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the optokinetic response, a computer-based visual acuity response test (OptoMotry 1.7.7, Cerebral Mechanics) was used in all cohorts of mice. Briefly, mice were tested for visual acuity by observing their optokinetic responses to rotating sinusoidal gratings (65 (link), 66 (link)). Mice were placed on a platform at the center of a virtual reality chamber composed of four monitors that display sine wave gratings rotating at 12°/s. Mice were monitored through a video camera, which was continuously recentered on the head of the animal, and the presence or absence of the optokinetic reflex was recorded. At the beginning, mice were allowed to settle on the platform for 3 to 5 min. To assess visual acuity, initially, the grating began at a 100% contrast with spatial frequency of 0.05 cycles/deg for both directions of rotation, which gradually increased until head turning was no longer observed. Spatial frequency threshold, a measure of visual acuity, was determined automatically with the Opto-kinetic tracking (OKT) software.
4
Optomotor Response Assay for Visual Acuity
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To evaluate the visual acuity, we used a computer-based optomotor response (OMR) test (OptoMotry 1.7.7; Cerebral Mechanics, Lethbridge, Alberta, Canada) as described earlier.20 (link) In brief, free moving mice were tested for visual acuity by observing head movements that track rotating sinusoidal gratings. These observations were made in real time, via a video camera projecting to the computer screen. The observer was masked to the genotype of mice. Mice were placed on the platform and allowed to settle for 5 minutes. In the rotating virtual drum, spatial frequency at 100% contrast was systematically increased in the staircase method until the mice were unable to track them. The highest spatial frequency each eye could track is reported as a measure of visual acuity.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!