Log In Sign up
The largest database of trusted experimental protocols

Pharmingen pi rnase staining buffer

Manufactured by BD
Visit Supplier
Sourced in United States

BD Pharmingen PI/RNase staining buffer is a solution used for preparing cell samples for flow cytometric analysis. It is designed to permeabilize cells and remove RNA, allowing for the staining and detection of cellular DNA content.

Automatically generated - may contain errors

Lab products found in correlation

Lsrfortessa x 20 flow cytometer, by BD (2 mentions) Pharmingen stain buffer, by BD (2 mentions) Facscanto flow cytometer, by BD (2 mentions) Facscalibur flow cytometer, by BD (1 mentions) Facs caliber flow cytometer, by BD (1 mentions) Pe annexin 5 apoptosis detection kit, by BD (1 mentions) Celltiter 96 non radioactive cell proliferation assay kit, by Promega (1 mentions) Xmark microplate spectrophotometer, by Bio-Rad (1 mentions) Cytofix fixation buffer, by BD (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Accuri c6 flow cytometer, by BD (1 mentions) Pharmingen staining buffer, by BD (1 mentions) Cellquest pro 6, by BD (1 mentions) Pe annexin 5 apoptosis detection kit 1, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Fitc annexin 5 apoptosis detection kit, by BD (1 mentions) Facsaria, by BD (1 mentions)

Spelling variants of this product

PI/RNase Staining Buffer (643 citations) Staining buffer (101 citations) PI/RNase (38 citations) PI/RNase buffer (23 citations) PI) staining buffer (11 citations) RNase staining buffer (9 citations) PI/RNase staining (6 citations) BD Pharmingen TM PI/RNase (5 citations) BD Pharmingen™ PI/RNase staining (4 citations) BD Pharmingen staining buffer (2 citations)

15 protocols using pharmingen pi rnase staining buffer

1

Cell Cycle Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
The cells were fixed with 70% ethanol at −20 °C overnight, then washed with PBS and resuspended in BD Pharmingen™ PI/RNase Staining Buffer (BD Biosciences). The cells were incubated at 37 °C for 40 min, and the cell cycle was detected by FACS Calibur Flow Cytometer (BD Biosciences). The results were processed by FlowJo10 software.
Huang X., Xiao S., Zhu X., Yu Y., Cao M., Zhang X., Li S., Zhu W., Wu F., Zheng X., Jin L., Xie C., Huang X., Zou P., Li X, & Cui R. (2020). miR-196b-5p-mediated downregulation of FAS promotes NSCLC progression by activating IL6-STAT3 signaling. Cell Death & Disease, 11(9), 785.
+ Open protocol
+ Expand
2

Annexin V and PI Assays for Apoptosis and Cell Cycle

Check if the same lab product or an alternative is used in the 5 most similar protocols
A PE Annexin V Apoptosis Detection Kit (BD Biosciences, Rockville, MD, USA) was applied to measure cell apoptosis. Cells were gently trypsinized and washed with cold PBS twice, resuspended in 500 μL Annexin V binding buffer; then 5 μL 7‐AAD and 2.5 μL Annexin V were added to each sample, which were incubated in the dark for 15 minutes at room temperature, and analyzed with a FACSCaliber flow cytometer (BD Biosciences). For cell cycle analysis, cells were washed with PBS twice and fixed with 70% ethanol at 4°C overnight. After centrifugation, cells were washed and resuspended in 500 μL BD Pharmingen PI/RNase staining buffer (BD Biosciences). Then cells were incubated for 15 minutes at room temperature and analyzed on the same flow cytometer.
He J., Jin Y., Zhou M., Li X., Chen W., Wang Y., Gu S., Cao Y., Chu C., Liu X, & Zou Q. (2018). Solute carrier family 35 member F2 is indispensable for papillary thyroid carcinoma progression through activation of transforming growth factor‐β type I receptor/apoptosis signal‐regulating kinase 1/mitogen‐activated protein kinase signaling axis. Cancer Science, 109(3), 642-655.
+ Open protocol
+ Expand
3

Cell Cycle Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and washed in PBS. Cells were fixed in cold 70% ethanol for at least 2 hours at −20 ℃. Cells were then washed twice in PBS and once in BD Pharmingen stain buffer (BD Biosciences, 554656). The supernatant was discarded after spinning down. Cell pellets were re-suspended in 0.5 ml of BD Pharmingen PI/RNase staining buffer (BD Biosciences, 550825) and incubated for 15 min at room temperature (RT), and cells were immediately analyzed using an LSRFORTESSA X-20 flow cytometer (BD Biosciences). The data was analyzed with the FlowJo software.
Fang Z., Wang Y., Wang Z., Xu M., Ren S., Yang D., Hong M, & Xie W. (2020). ERINA is an estrogen-responsive lncRNA that drives breast cancer through the E2F1/RB1 pathway. Cancer research, 80(20), 4399-4413.
+ Open protocol
+ Expand
4

MTT Assay and Cell Cycle Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were seeded at 2,000 cells per well in 96-well culture plates, and MTT assays were performed with a CellTiter 96 Non-Radioactive Cell Proliferation Assay Kit (Promega, #G4100) following the manufacturer’s guidelines. The absorbance value was measured at 570 nm using an xMark Microplate Spectrophotometer (Bio-Rad) with a reference wavelength of 630 nm.
For the cell cycle assay, cells were collected, rinsed with PBS, and fixed for a minimum of 2 hr by adding 70% ice-cold ethanol at −20°C. Cells were then sequentially washed once in PBS and BD Pharmingen stain buffer (BD Biosciences, #554656). Cell pellets were resuspended in 0.5 ml of BD Pharmingen PI/RNase staining buffer (BD Biosciences, #550825) and incubated for 15 min at room temperature (RT), and cells were immediately analyzed using an LSRFORTESSA X-20 flow cytometer (BD Biosciences). The data were analyzed with FlowJo software.
Wang Z., Yang B., Zhang M., Guo W., Wu Z., Wang Y., Jia L., Li S., Xie W, & Yang D. (2018). LncRNA epigenetic landscape analysis identifies EPIC1 as an oncogenic lncRNA that interacts with MYC and promotes cell cycle progression in cancer. Cancer cell, 33(4), 706-720.e9.
+ Open protocol
+ Expand
5

Flow Cytometry Cell Fixation and Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed and stained for flow cytometry using BD Cytofix fixation buffer (554655, BD Biosciences, Franklin Lakes, New Jersey) and BD Pharmingen™ PI/RNase staining buffer (550825, BD Biosciences), following the manufacturer’s instructions. Side versus forward scatter plots obtained on a BD Biosciences LSRII flow cytometer are shown.
Lopez-Camacho C., van Wijnen A.J., Lian J.B., Stein J.L, & Stein G.S. (2014). Core Binding Factor β (CBFβ) and the Leukemogenic Fusion Protein CBFβ-Smooth Muscle Myosin Heavy Chain (SMMHC) Associate with Mitotic Chromosomes to Epigenetically Regulate Ribosomal Gene Expression. Journal of cellular biochemistry, 115(12), 2155-2164.
+ Open protocol
+ Expand
6

Cell Cycle Analysis in LX2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
LX2 cells were seeded in 6-well plates at 3 × 105 cells per well. After transfection for 48 h, cells were trypsinized and fixed in 70% ethanol at -20°C for 24 h. Then, cells were stained using BD Pharmingen PI/ RNase staining buffer (BD Biosciences, United States). The cell cycle distribution was analyzed using an Accuri C6 flow cytometer (BD Biosciences, United States) and ModFit LT software.
Wu J.C., Chen R., Luo X., Li Z.H., Luo S.Z, & Xu M.Y. (2019). MicroRNA-194 inactivates hepatic stellate cells and alleviates liver fibrosis by inhibiting AKT2. World Journal of Gastroenterology, 25(31), 4468-4480.
+ Open protocol
+ Expand
7

Cell Cycle Analysis by PI Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in 75% ethanol overnight and then stained with propidium iodide (PI) using BD Pharmingen™ PI/RNase Staining Buffer (BD, Biosciences) according to the manufacturer's protocol. The percentages of cells in G0/G1, S and G2/M phase were obtained and analysed using Wincycle‐32 bit.
Du X., Tu Y., Liu S., Zhao P., Bao Z., Li C., Li J., Pan M, & Ji J. (2019). LINC00511 contributes to glioblastoma tumorigenesis and epithelial‐mesenchymal transition via LINC00511/miR‐524‐5p/YB1/ZEB1 positive feedback loop. Journal of Cellular and Molecular Medicine, 24(2), 1474-1487.
+ Open protocol
+ Expand
8

Cell Cycle Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Harvested cells were washed and fixed in cold ethanol (70%) for 2 h at −20°C. Then, cells were washed twice using PBS and once in a BD Pharmingen staining buffer (BD Biosciences, 554,656). Resuspension of cell pellets was done in BD Pharmingen PI/RNase staining buffer (0.5 ml; BD Biosciences, 550,825) followed by 15 min of incubation at room temperature (RT). Detection of red fluorescence was done at an excitation wavelength of 488 nm, after which the abundance of cells at G0/G1, S, as well as G2/M phases were determined by the ModFit software.
Qu Y., He Y., Ruan H., Qin L, & Han Z. (2022). Abnormal downregulation of 10‐formyltetrahydrofolate dehydrogenase promotes the progression of oral squamous cell carcinoma by activating PI3K/Akt/Rb pathway. Cancer Medicine, 12(5), 5781-5797.
+ Open protocol
+ Expand
9

Cell Fixation and PI Staining

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed with PBS twice and fixed with 70% ethanol at 4°C overnight. After centrifugation, cells were washed and resuspended in 500 μL BD Pharmingen PI/RNase staining buffer (BD Biosciences). Then cells were incubated for 15 minutes at room temperature and analyzed on the same flow cytometer.
Qin S., Ning M., Liu Q., Ding X., Wang Y, & Liu Q. (2021). Knockdown of long non-coding RNA CDKN2B-AS1 suppresses the progression of breast cancer by miR-122-5p/STK39 axis. Bioengineered, 12(1), 5125-5137.
+ Open protocol
+ Expand
10

Cell Cycle Analysis by Flow Cytometry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells in a culture dish were collected after 24 h, washed twice in ice-cold PBS, and fixed in 75% ice-cold ethanol in PBS. The cells were washed twice in PBS, then stained with BD Pharmingen™ PI/RNase Staining Buffer (BD Biosciences, San Diego, CA, USA) for 30 min at 4°C. The cell suspension was subjected to flow cytometry (BD Biosciences).
Li W., Wang S., Shan B., Cheng X., He H., Qin J., Tang Y., Zhao H., Tian M., Zhang X, & Jin G. (2021). CircHECTD1 Regulates Cell Proliferation and Migration by the miR-320-5p/SLC2A1 Axis in Glioblastoma Multiform. Frontiers in Oncology, 11, 666391.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!