Electron paramagnetic resonance (EPR) spectra of H64D Mb (0.3 mM, in 100 mM KPi buffer, pH 6.0) and A15C/H64D Ngb (0.3 mM, in 100 mM KPi buffer, pH 7.0) in the absence or presence of MG (0.3 mM) were recorded on a Bruker A300 spectrometer (X-band) equipped with Bruker ER4141VTM liquid nitrogen system. The sample was transferred into an EPR tube with a volume of 300 μL. The spectrum was measured at 100 K, with a frequency of 9.27 GHz, centerfield 2750 G and sweep width 4500 G, microwave power 0.595 mW, and modulation amplitude 3.0 G.
Er4141v liquid nitrogen system
Manufactured by Bruker
The ER4141VTM is a liquid nitrogen system designed for use with Bruker's electron paramagnetic resonance (EPR) spectrometers. It provides a regulated and stabilized flow of liquid nitrogen to the sample cavity, maintaining the necessary cryogenic temperatures for EPR experiments. The system is engineered to ensure reliable and consistent performance for EPR applications.
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3 protocols using er4141v liquid nitrogen system
1
EPR Analysis of Heme Protein Variants
2
Structural and Morphological Analysis of Samples
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The morphology and structure of the sample was investigated by transmission electron microscopy (TEM, Hitachi HT 7700) and high-resolution TEM (HRTEM, JEOL-2010). X-ray diffraction patterns were obtained using a Bruker D8 Advance with Cu-Kα radiation. X-ray photoelectron spectra (XPS) of the samples were recorded on an ESCALAB 250. The thermogravimetric analysis (TGA) was carried out on SDT 2960 with a heating rate of 10 °C min−1 from 20 to 600 °C. The BET surface area was determined on an ASAP 2460 sorption apparatus. All the as-prepared samples were degassed at 150 °C for 10 h prior to nitrogen adsorption measurements. Electron paramagnetic resonance (EPR) tests were carried out on a Bruker A300 spectrometer (X-band, frequency 9.43 GHz) equipped with Bruker ER4141VTM liquid nitrogen system. The microwave power was 0.595 mW and modulation amplitude 3.0 G. The samples were measured at 90 K with center field 3500 G and sweep width 5000 G.
3
EPR Spectroscopy of Myoglobin Mutants
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Electron paramagnetic resonance (EPR) spectra of F43H/H64A Mb (0.5 mM, in 100 mM KPi buffer, pH 6.0) in the absence or presence of MG (1 mM) were recorded on a Bruker A300 spectrometer (X-band) equipped with Bruker ER4141VTM liquid nitrogen system. The sample was transferred into an EPR tube with a volume of 300 μL. The spectrum was measured at 100 K, with frequency of 9.43 GHz, center field 2200 G and sweep width 3600 G, microwave power 0.595 mW and modulation amplitude 3.0 G.
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