Ma1 38223

Cells were fixed with 4% paraformaldehyde (PFA) in PBS for 10 min and permeabilized with 0.5% Triton X-100 (Sigma-Aldrich) in PBS for 5 min at room temperature. Cells were stained with the primary antibodies for 1 h and secondary antibodies for 45 min each at room temperature. DNA was counterstained with DAPI. Primary antibodies included the following: TP63 (1:100, CM163A; Biocare Medical), TTF1 (1:200, ab76013; Abcam), SOX2 (1:200, 09-0024; ReproCELL USA Inc., Beltsville, MD, USA), PE-conjugated human EpCAM (1:200, 12-9326-42; Thermo Fisher Scientific), MUC5AC (1:200, MA1-38223; Thermo Fisher Scientific), APC-conjugated human CD47 (1:20, 323123; Biolegend), PE-conjugated human CD26 (1:20, 302705; Biolegend, San Diego, CA, USA), Alexa Fluor 647-conjugated human Podoplanin (1:200, 337007; Biolegend), and Alexa Fluor 488-conjugated human SP-C (1:200, sc-518029AF488, Santa Cruz Biotechnology, Dallas, TX, USA). Secondary antibodies included Alexa Fluor 488 goat anti-mouse IgG (H+L) secondary antibody (1:200, A11029; Thermo Fisher Scientific), Alexa Fluor 568 donkey anti-rabbit IgG (H+L) secondary antibody (A10042; Thermo Fisher Scientific), and Alexa Fluor 594 goat anti-mouse IgG (H + L) secondary antibody (A11032; Thermo Fisher Scientific).