Gold labeled goat anti mouse igg antibody

The mouse anti-FLAG (Sigma-Aldrich, USA), mouse anti-Myc (Sigma-Aldrich, USA), rabbit anti-ATP6V1A (Sigma-Aldrich, USA), mouse anti-β-actin (Proteintech, China), mouse anti-HA epitope tag (Proteintech, China), mouse anti-GST (Sigma-Aldrich, USA), mouse anti-His epitope tag (Proteintech, China), rabbit IgG (Solarbio, China) and the secondary antibodies (HRP-conjugated anti-mouse IgG antibody [Sigma-Aldrich, USA], HRP-conjugated anti-rabbit IgG antibody [Genescript, China], FITC-conjugated goat anti-mouse IgG antibody [ZSGB. Bio, China], Alexa Fluor 647-labeled goat anti-rabbit IgG (H + L) [Beyotime, China]) and gold-labeled goat anti-mouse IgG antibody (Sigma-Aldrich, USA) were purchased from the indicated vendors. Murine sera against M, P, or G protein were produced in our laboratory.

The cRVLPs were applied to grids, stained with 1% sodium phosphotungstate, and then observed using transmission electron microscopy. The cRVLPs were also bound to formvar-coated grids, incubated with mouse anti-flagellin or anti-LTB antibodies, and then incubated with gold-labeled goat anti-mouse IgG antibody (Sigma-Aldrich, Saint Louis, MO, USA). Finally, the grids were negatively stained and observed.