LC-MS/MS was performed using a Waters series 2695 HPLC instrument coupled with an amaZon SL ion trap Mass spectrometer (Bruker, Karlsruhe, Germany), with a Xchange C18 column (Acchrom Co., CO, USA) 250 mm × 4.6 mm, 5 μm, 0.5 mL/min). The organic portion was dissolved in MeOH at 10 mg/mL, filtered through a Gracepure C18 SPE cartridge and analyzed by LC–MS/MS. Ten μL aliquot of each sample was injected and eluted with a gradient program of MeOH-H2O (0.1% formic acid) (0–20 min 10–100%, 21–25 min 100%; 1.0 mL/min). Mass spectra were obtained in positive ESI mode and with an automated fully dependent MS/MS scan from 100 to 1000 Da. MS/MS data were converted digitally to mzXML files using Filezilla software. The molecular networking was performed using the GNPS data analysis workflow using the spectral clustering algorithm [28 (link)]. The spectral networks were imported into Cytoscape 3.9.1 and visualized using the force-directed layout.
Amazon sl ion trap mass spectrometer
Manufactured by Bruker
Sourced in Germany, United States
The AmaZon SL ion trap mass spectrometer is a versatile analytical instrument designed for high-performance mass spectrometry. It utilizes an ion trap technology to efficiently capture, store, and analyze ions, enabling sensitive and accurate measurements of a wide range of compounds. The AmaZon SL provides robust and reliable performance for various applications in analytical chemistry, life sciences, and materials research.
Automatically generated - may contain errors
Lab products found in correlation
Compass dataanalysis 4, by Bruker (5 mentions)
Maxis uhr esi time of flight mass spectrometer, by Bruker (4 mentions)
Uhplc 3000 rs system, by Thermo Fisher Scientific (4 mentions)
F 4600 fluorescence spectrophotometer, by Hitachi (3 mentions)
Kinetex xb c18 column, by Phenomenex (3 mentions)
Ultimate 3000 series system, by Thermo Fisher Scientific (3 mentions)
Av 400 spectrometer, by Bruker (2 mentions)
Avance 3 600 mhz spectrometer, by Bruker (2 mentions)
Avance 3 500 mhz spectrometer, by Bruker (2 mentions)
2400 chn elemental analyzer, by PerkinElmer (2 mentions)
Ultimate 3000 rslc system, by Thermo Fisher Scientific (2 mentions)
F 4600 spectrophotometer, by Hitachi (2 mentions)
8453 uv vis spectrophotometer, by Agilent Technologies (2 mentions)
Av400 400 mhz spectrometer, by Bruker (2 mentions)
Data analysis software, by Bruker (2 mentions)
Esi l tuning mix, by Agilent Technologies (2 mentions)
Advance 3 nmr spectrometer, by Bruker (1 mentions)
Agilent 1260 infinity hplc series, by Agilent Technologies (1 mentions)
400 mhz or 500 mhz spectrometer, by Bruker (1 mentions)
Spectrum two ft ir spectrometer, by PerkinElmer (1 mentions)
43 protocols using amazon sl ion trap mass spectrometer
1
LC-MS/MS Analysis of Organic Compounds
2
Characterization of Novel Photosensitive Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the chemical reagents used in the experiments were purchased from commercial sources and were not purified further prior to use. The solutions of metal ions (0.1 mol L−1) used in the tests were prepared by dissolving the corresponding metal nitrates in distilled water except for K+, Ba2+, Hg2+ and Sn2+ (their counter ions were chloride ions). The NMR spectra were measured with tetramethylsilane (TMS) as the internal standard on a Bruker AV400 spectrometer. Mass spectra were recorded with a Bruker Amazon SL ion trap mass spectrometer (ESI) using methanol as the solvent. The melting points were determined on a WRS-1B melting point apparatus. The absorption spectra and fluorescence spectra were collected on an Agilent 8454 UV/vis spectrometer and a Hitachi F-4600 fluorescence spectrophotometer, respectively. Moreover, MUA-165 UV lamp and MVL-210 visible lamp were used for photoirradiation. The fluorescence quantum yield was determined on an Absolute PL Quantum Yield Spectrometer QYC11347-11. Infrared spectra (IR) were collected on a BrukerVertex-70 spectrometer. Unless otherwise indicated, all measurements were made at room temperature, and the sample concentration was maintained at 2.0 × 10−5 mol L−1.
3
Purification and Characterization of Punicalagin
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Tertiary fraction 8, produced by HPLC–UV/MS, was further purified on an Agilent 1260 Infinity Series HPLC (Agilent Technologies, Santa Clara, CA, USA) with an online SPE solution in combination with a Bruker amazon SL IonTrap mass spectrometer (Bruker Daltonics, Bremen, Germany). A 13 mg/mL solution was prepared and a Luna® C18(2) (250 × 4.6 mm, 5 μm) column was used for separation together with Oasis® On-Line SPE trapping cartridges for collection. The following gradient method was used with H2O (0.1% FA) and MeOH (0.1% FA) as the mobile phases with a 12-µL injection volume and a flow rate of 0.5 mL/min: 20% MeOH isocratic hold for 15 min, linear increase to 100% MeOH at 15.5 min, an isocratic hold at 100% MeOH until 20 min, before returning to starting conditions at 23 min. A 5-min column equilibration phase with the starting conditions was incorporated before the subsequent injection.
Mass-directed fractionation was used in ESI negative mode and peaks corresponding to 541 g/mol (λmax = 217 nm, 258 nm, and 378 nm) were trapped.
Punicalagin was isolated and analysed on a 400 MHz Bruker Advance III NMR spectrometer at 25 °C. The compound was dissolved and analysed in deuterated acetone-d6 (Sigma-Aldrich, Milwaukee, WI, USA). The resulting spectrum was compared to that published in literature [23 ].
Mass-directed fractionation was used in ESI negative mode and peaks corresponding to 541 g/mol (λmax = 217 nm, 258 nm, and 378 nm) were trapped.
Punicalagin was isolated and analysed on a 400 MHz Bruker Advance III NMR spectrometer at 25 °C. The compound was dissolved and analysed in deuterated acetone-d6 (Sigma-Aldrich, Milwaukee, WI, USA). The resulting spectrum was compared to that published in literature [23 ].
4
Spectroscopic Characterization of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The specific optical rotation of compounds ([α]) was measured using an Optical Activity Limited AA65 Automatic Polarimeter, analytical version (589 nm), with a path length of 1.0 dm, with concentrations (c) quoted in g 100 ml-1. IR spectra were collected using UATR Two, PerkinElmer Spectrum Two FT-IR spectrometer over the range 4000–400 cm-1. Low resolution ESI mass spectrometry (LRMS) was performed using a Bruker amaZon SL ion trap mass spectrometer. High resolution ESI mass spectra (HRMS) were collected on a Bruker FTICR mass spectrometer. 1H and 13C NMR spectra were obtained at 300 K on a Bruker 400 MHz or 500 MHz spectrometer.
5
Analytical Procedures for Compound Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All solvents and reagents were
obtained from commercial suppliers and used without further purification.
Elemental analyses were performed on a Perkin Elmer 2400 CHN elemental
analyzer at the Microanalytical Laboratory of the University of Vienna
and are within ±0.4%, confirming >95% purity. Electrospray
ionization
(ESI) mass spectra were recorded on a Bruker amaZon SL ion trap mass
spectrometer in positive mode by direct infusion. High-resolution
mass spectra were measured on a Bruker maXis UHR ESI time of flight
mass spectrometer. One-dimensional 1H NMR spectra of the
precursors were recorded on a Bruker Avance III 500 MHz spectrometer
at 298 K. One- and two-dimensional 1H NMR and 13C NMR spectra of the final products were recorded on a Bruker Avance
III 600 MHz spectrometer at 298 K. For 1H NMR spectra,
the solvent residual peak was taken as an internal reference (s =
singlet, d = doublet, t = triplet, quint = quintet, dd = doublet of
doublets, ddd = doublet of doublet of doublets, br = broad signal,
m = multiplet, py = pyridine). For the NMR numbering scheme of the
compounds, see Supporting InformationFigures S8 and S9 .
obtained from commercial suppliers and used without further purification.
Elemental analyses were performed on a Perkin Elmer 2400 CHN elemental
analyzer at the Microanalytical Laboratory of the University of Vienna
and are within ±0.4%, confirming >95% purity. Electrospray
ionization
(ESI) mass spectra were recorded on a Bruker amaZon SL ion trap mass
spectrometer in positive mode by direct infusion. High-resolution
mass spectra were measured on a Bruker maXis UHR ESI time of flight
mass spectrometer. One-dimensional 1H NMR spectra of the
precursors were recorded on a Bruker Avance III 500 MHz spectrometer
at 298 K. One- and two-dimensional 1H NMR and 13C NMR spectra of the final products were recorded on a Bruker Avance
III 600 MHz spectrometer at 298 K. For 1H NMR spectra,
the solvent residual peak was taken as an internal reference (s =
singlet, d = doublet, t = triplet, quint = quintet, dd = doublet of
doublets, ddd = doublet of doublet of doublets, br = broad signal,
m = multiplet, py = pyridine). For the NMR numbering scheme of the
compounds, see Supporting Information
6
Comprehensive UHPLC-DAD-MS^n Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
UHPLC-DAD-MSn analysis was performed on UHPLC-3000 RS system (Dionex, Germany) with DAD detection and an AmaZon SL ion trap mass spectrometer with ESI interface (Bruker Daltonik GmbH, Germany). Separation was performed on a Kinetex XBC18 column (150 × 2.1 mm, 1.7 μm) Phenomenex (Torrance, CA, USA). The column temperature was 25 °C. The mobile phase (A) was H2O/HCOOH (100:0.1, v/v) and the mobile phase (B) was MeCN/HCOOH (100:0.1, v/v). A gradient system was used: 0–10 min 10–25% B; 10–40 min 25–35% B. The flow rate was 0.3 mL min−1. The column was equilibrated for 7 min between injections. UV spectra were recorded over a range of 200–450 nm; chromatograms were acquired at 325 nm. The LC eluate was introduced directly into the ESI interface without splitting. The nebulizer pressure was 40 psi; dry gas flow 9 L min−1; dry temperature 300 °C; and capillary voltage 4.5 kV. Analysis was carried out using scan from m/z 90 to 2,200. Compounds were analyzed in negative ion mode. The MS2 fragmentation was obtained for the most abundant ion at the time.
7
UHPLC-MS Analysis of Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromatographic separation was performed on a Hitachi LaChrom Elite HPLC ultra-high-pressure liquid chromatography (U-HPLC) system (Hitachi High-Technologies Corporation, Tokyo, Japan) fitted with a Bruker amaZon SL Ion Trap Mass Spectrometer. The Chromatographic separation was performed on an Inertsil ODS-3 150 mm × 4.6 mm, 5 μm (GL Sciences Inc., Tokyo, Japan). The mobile phase was composed of water (A) with formic acid solution (pH = 3.0) and 100% acetonitrile (B) with formic acid solution (pH = 3.0) under gradient elution conditions at 0–60 min, 0–50% B; 60–90 min, 50–100% B and 90–100 min, 100% B. The flow rate of the mobile phase was 0.4 ml/min, and the column temperature was maintained at 30°C. Mass spectrum analysis was performed using a Bruker amaZon SL Ion Trap Mass Spectrometer (Bruker, Yokohama, Japan) fitted with an ESI source and operated in negative ion mode. The key optimized ESI parameters were as follows: spray voltage: -3.5 KV; capillary temp: 220°C; Ultra scan. Data were collected with Bruker Compass Data Analysis 4.2 (Bruker, Yokohama, Japan) and analyzed by Mass++ software [31 ] with the MassBank mass spectral library.
8
Synthesis and Characterization of COTI-2 and COTI-NH2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
COTI-2 and COTI-NH2 were prepared as described previously [16 (link)]. All solvents and reagents were obtained from commercial suppliers and used without further purification. A Perkin Elmer 2400 CHN Elemental Analyzer at the Microanalytical Laboratory of the University of Vienna was used for elemental analyses. All values were within ±0.4%, confirming >95% purity. Electrospray ionization (ESI) mass spectra were obtained using a Bruker amaZon SL ion trap mass spectrometer in the positive mode by direct infusion, while high-resolution mass spectra were obtained on a Bruker maXis UHR ESI time-of-flight mass spectrometer. One-dimensional 1H-NMR spectra of the precursors were recorded on a Bruker Avance III 500 MHz spectrometer at 298 K. One- and two-dimensional 1H-NMR and 13C-NMR spectra of the final products were recorded on a Bruker Avance III 600 MHz spectrometer at 298 K. For 1H-NMR spectra the solvent residual peak was taken as yjr internal reference (s = singlet; d = doublet; t = triplet; quint = quintet; dd = doublet of doublets; ddd = doublet of doublet of doublets; br = broad signal; m = multiplet; py = pyridine). For the NMR numbering of the compounds, see Scheme S1 and Scheme S2 .
9
Synthesis and Characterization of K2[PtCl4]
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Potassium tetrachloridoplatinate (K2[PtCl4]) was purchased from Johnson Matthey (Switzerland). Water for synthesis was taken from a reverse osmosis system. For HPLC measurements Milli-Q water (18.2 MΩ cm, Merck Milli-Q Advantage, Darmstadt, Germany) was used. Other chemicals and solvents were purchased from commercial suppliers (Sigma Aldrich, Merck and Fisher Scientific). Electrospray ionization (ESI) mass spectra were recorded on a Bruker Amazon SL ion trap mass spectrometer in positive and/or negative mode by direct infusion. High resolution mass spectra were measured on a Bruker maXis™ UHR ESI time of flight mass spectrometer. One- and two-dimensional 1H-NMR and 13C-NMR spectra were recorded on a Bruker Avance III 500 or AV III 600 spectrometer at 298 K. For 1H-NMR spectra the solvent residual peak was taken as internal reference. Elemental analysis measurements were performed on a PerkinElmer 2400 CHN Elemental Analyzer at the Microanalytical Laboratory of the University of Vienna. The compounds were purified by preparative RP-HPLC using a Waters XBridge C18 column on an Agilent 1200 Series system. Milli-Q water and acetonitrile were used as eluents with a flow rate of 17 ml min−1, unless otherwise stated.
10
UHPLC-MS Analysis of Phytochemicals
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chromatographic analysis was performed on a UHPLC-3000 RS system (Dionex, Germany) with a diode-array detector (DAD) and an AmaZon SL ion trap mass spectrometer with an ESI interface (Bruker Daltonik GmbH, Bremen, Germany). A Kinetex XB-C18 column (150 × 2.1 mm, 1.7 μm) (Phenomenex, Torrance, CA, USA) set at the temperature of 25 °C was used for separation. A mobile phase A was 0.1% formic acid in the water, and mobile phase B was 0.1% formic acid in acetonitrile. The gradient program with a flow rate 0.2 mL/min, used for the separation of phytochemicals, was as follows: 4–26% B, 0–60 min; 26–95% B, 60–90 min.; 4% (equilibration), 90–100 min. The UV chromatograms were registered at 240, 280, 325 nm, or 520 nm. The conditions of ESI parameters were described previously [22 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!