Icosl

Manufactured by BioLegend

Sourced in United States

ICOSL is a cell surface protein that functions as a co-stimulatory molecule. It is expressed on antigen-presenting cells and plays a role in the activation of T cells.

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Lab products found in correlation

Desmoglein 2, by Santa Cruz Biotechnology (1 mentions) Ox40l, by BD (1 mentions) Gallios flow cytometer, by BD (1 mentions) Kaluza software, by BD (1 mentions) 7 aminoactinomycin d 7 aad, by BD (1 mentions) Ionomycin, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate, by Thermo Fisher Scientific (1 mentions) Cd62l, by Thermo Fisher Scientific (1 mentions) Type 2 collagen cii, by Chondrex (1 mentions) Interleukin 4 (il 4), by BioLegend (1 mentions) Il 22, by BioLegend (1 mentions) Hyaluronidase, by Merck Group (1 mentions) Ifn γ, by BioLegend (1 mentions) Streptavidin alexa 488, by Thermo Fisher Scientific (1 mentions) Streptavidin alkaline phosphatase, by Southern Biotech (1 mentions) Anti igg alkaline phosphatase, by Southern Biotech (1 mentions) Np osu, by Biosearch Technologies (1 mentions) Pd l1, by BioLegend (1 mentions) Anti igm f ab 2, by Jackson ImmunoResearch (1 mentions) Mhc class 2, by Thermo Fisher Scientific (1 mentions)

4 protocols using icosl

Characterization of Immune Cell Populations

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Type II collagen (CII) was purchased from Chondrex (Redmond, WA, USA). Hyaluronidase was obtained from Sigma-Aldrich (St. Louis, MO, USA). Phorbol 12-myristate 13-acetate (PMA) and ionomycin were all products from eBioscience (San Diego, CA, USA). Fluorochrome-conjugated anti-human monoclonal antibodies (mAbs) including CD4 (clone: RPA-T4), CD14 (clone:M5E2), CD19 (clone: HIB19), ICOS (clone:C398.4A), ICOSL (clone:2D3) were from Biolegend (San Diego, CA, USA). Fluorochrome-conjugated anti-mouse mAbs including CD3 (clone:145-2C11), CD19 (clone:6D5), NK1.1 (clone:PK136), ICOS (clone:15F9), ICOSL (clone:HK5.3), CD44 (clone:IM7), B220 (clone:RA3-6B2), CD138 (clone:281-2), GL7 (clone:GL7), CD25 (clone:3C7), Foxp3 (clone:150D), CXCR5 (clone:L138D7), IFN-γ (clone:XMG1.2), TNF-α (clone:MP6-XT22), IL-4 (clone:11B11), IL-9 (clone:RM94A), IL-17 (clone:TC11-18H10.1) and IL-22 (clone:poly5164) were from Biolegend. Fluorochrome-conjugated anti-mouse mAbs CD4 (clone: GK1.5), CD38 (clone:90), CD95 (clone:15A7), CD62L (clone: MEL-14), IL-6 (clone:MP5-20F3), IL-21 (clone: FFA21) were from eBioscience. Anti-mouse CD11b mAb was from Mitenyi Biotec (Bergisch Gladbach, Germany). The anti-mouse ICOSL blocking antibody (clone HK5.3) was from Biolegend.

Ding S., Sun Z., Jiang J., Chang X., Shen Y., Gu Y, & Liu C. (2022). Inducible costimulator ligand (ICOSL) on CD19+ B cells is involved in immunopathological damage of rheumatoid arthritis (RA). Frontiers in Immunology, 13, 1015831.

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Monoclonal Antibody Staining for Flow Cytometry

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The following monoclonal antibodies conjugated with fluorochrome were used: Desmoglein-2(Santa Cruz Biotechnology), 41BBL, ICOSL (BioLegend), OX40L, CAR, CD46 (BD Biosciences), FAP (R&D Systems). Cells were stained with these antibodies for 30 min at 4 °C. Live/dead discrimination was determined via inclusion of 7-aminoactinomycin D (7AAD; BD Pharmingen). Stained cells were analyzed using Gallios flow cytometer with Kaluza software (BD Biosciences) according to the manufacturer’s instructions.

Rosewell Shaw A., Porter C., Biegert G., Jatta L, & Suzuki M. (2022). HydrAd: A Helper-Dependent Adenovirus Targeting Multiple Immune Pathways for Cancer Immunotherapy. Cancers, 14(11), 2769.

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Antibody Reagents for B Cell Immunology

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Antibodies against mouse CD4, CD19, CD95, GL-7, ICOS, ICOSL, PD-1, and B220-647 were purchased from Biolegend, CXCR5, B220, IgG₁, IgG_2a, IgG_2b, and IgG₃ antibodies from BD Biosciences, Bcl-6, XBP-1, and IRF-4 from Santa Cruz, and BAFF (1C9) from Enzo. Anti-μ F(ab)₂ was purchased from Jackson ImmunoResearch. Anti-μ (clone B7.6), anti-NP (clones H33L and B1-8), 2.4G2 (FcγRIIb /FcγRIII block) and Ac38 idiotype antibodies were purified from hybridoma supernatants. (Ac38 is an idiotype antibody that recognizes B-1-8 specificities generated during NP immunization). Recombinant mouse BR3-Fc and isotype control protein were generated using mammalian expression systems and standard purification protocols. H33L and B1-8 were gifts from Dr. Garnett Kelsoe (Duke University). IL-4 and IL-5 were purchased from Peprotech, recombinant BAFF (rBAFF) from R&D Systems, NP-OSu from Biosearch Technologies, KLH and PNA-biotin from Sigma Aldrich, alum from Thermo Scientific, and streptavidin-Alexa 488 and Alexa 647 from Invitrogen. Streptavidin-alkaline phosphatase and anti-IgG alkaline phosphatase were purchased from Southern Biotech. The Fc-binding TG19320 peptide was synthesized as described (39 (link), 40 (link)).

Kang S., Keener A.B., Jones S.Z., Benschop R.J., Caro-Maldonado A., Rathmell J.C., Clarke S.H., Matsushima G.K., Whitmire J.K, & Vilen B.J. (2015). IgG-immune complexes promote B cell memory by inducing BAFF. Journal of immunology (Baltimore, Md. : 1950), 196(1), 196-206.

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Costimulatory Marker Profiling in Murine B Cells

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IDO2 wt and ko C57BL/6 spleen cells were harvested and stimulated for 72h with anti-IgM F(ab′)₂ (Jackson Immunoresearch, 20μg/ml) or anti-CD40 (eBioscience, 2μg/ml) + IL-21 (eBioscience, 100ng/ml) in IMDM + 10% FCS + 5μM 2-mercaptoethanol, 2mM glutamax (Gibco), and 50μg/ml gentamycin. Levels of the following costimulatory markers were directly measured by flow cytometry on a BD FACSCanto with subsequent analysis using FlowJo Software (TreeStar): CD40 (eBioscience), CD80 (eBioscience), CD86 (eBioscience), ICOSL (BioLegend), IL-21R (BD Biosciences), OX40L (BioLegend), PDL1 (BioLegend), and MHC Class II (eBioscience). A minimum of 3 replicate mice were analyzed per condition.

Merlo L.M., DuHadaway J.B., Grabler S., Prendergast G.C., Muller A.J, & Mandik-Nayak L. (2016). IDO2 modulates T cell-dependent autoimmune responses through a B cell-intrinsic mechanism. Journal of immunology (Baltimore, Md. : 1950), 196(11), 4487-4497.

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