Agilent 6890 series

The FAME was analyzed by gas chromatography using an Agilent 6890 Series chromatograph (Agilent Technologies, Santa Clara, CA, USA) equipped with a split-splitless injector operating in split mode with a 20:1 split ratio at 250 °C, a SGE BPX70 column, 25 m × 0.32 mm internal diameter, a film thickness of 0.25 µm (Trajan Scientific, Australia), and a flame ionization detector at 280 °C with hydrogen at 40 mL/min, air at 450 mL/min, and nitrogen as the auxiliary gas at 25 mL/min. The analyses were run using nitrogen as the carrier gas at 10 psi. The oven temperature was held for 5 min at 100 °C, followed by an increment of 4 °C/min up to 240 °C, and then held for 20 min. A calibration curve for caprylic acid (R² = 0.99) was separately constructed to quantify its concentration.

Target PCBs were determined using gas chromatograph (Agilent 6890 Series, Agilent, Palo Alto, CA) equipped with micro electron capture detector. Separation was performed on a DB-5 capillary column (60 m length, 0.25 mm i.d., 0.25 μm film thickness, J&W Scientific, USA). The initial oven temperatures was set at 80 °C for 2 min then increased to 185 °C at a rate of 30 °C min⁻¹ (3 min), then at 1.5 °C min⁻¹ to 230 °C (held for 15 min) and finally to 270 °C at a rate of 5 °C min⁻¹ (held for 25 min). Nitrogen as carrier gas was used at 1.8 mL min⁻¹ flow rate. Injector was set at 270 °C and split-less mode. Detector was set at 300 °C. Confirmation of target PCB congeners were carried out using a Varian Gas Chromatograph CP-3800 (Varian, CA, USA) coupled to ion trap mass spectrometry detector (Varian Saturn 2000, Varian, CA, USA). The mass spectrums were acquired in MS/MS mode. 2 μL of each sample was injected in a programmable temperature vaporizing (PTV) injector (split-less mode). A VF-5MS capillary column (55 m length, 0.25 mm i.d. and 0.25 μm film thicknesses, Factor Four ®, Varian, Palo Alto, CA, USA) was employed. The GC oven was programmed as: initial temperature 100 °C for 2 min then to 200 °C (held for 3 ° min) at a rate of 30 °C min⁻¹ and then to 230 °C (held for 15 min) at a rate of 3 °C min⁻¹ and finally to 270 °C (held for 15 min) at a 5 °C min⁻¹ [18 (link), 19 (link)].

LC-MS/MS analyses of DIMBOA-Glc β-glucosidase activity were performed as described in (Wouters et al., 2014 (link)). GC-MS analyses were performed in an Agilent 6890 series gas chromatograph (Agilent Technologies, Waldbronn, Germany) using an Agilent 19091S-433 capillary column (30 m × 0.25 mm × 0.25 μm), using 2 μL injection on splitless mode at 200°C. The temperature program was: 40°C for 3 min, a 10°C^∗min⁻¹ ramp to 130°C, a 60°C^∗min⁻¹ ramp to 300°C, held for 3 min. The total running time was 17.83 min.