Facscan

Manufactured by Beckman Coulter

Sourced in United States

The FACScan is a flow cytometry instrument manufactured by Beckman Coulter. It is designed to analyze and sort cells based on their physical and fluorescent characteristics. The FACScan utilizes a laser to excite fluorescent labels within the sample, and then measures the emitted light signals to gather data on the cells.

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Lab products found in correlation

Rnase a, by Merck Group (25 mentions) Propidium iodide, by Merck Group (4 mentions) Annexin 5 fitc pi, by Thermo Fisher Scientific (4 mentions) Fitc annexin 5 apoptosis detection kit, by BD (3 mentions) Cell counting kit 8 (cck8), by Dojindo Laboratories (2 mentions) Annexin 5, by BD (2 mentions) Fitc conjugated annexin 5 staining, by Beyotime (2 mentions) Annexin 5 fitc pi apoptosis detection kit, by Beyotime (2 mentions) Fitc annexin 5 apoptosis detection kit 1, by BD (2 mentions) Annexin 5 pe and 7 aad, by BD (2 mentions) Propidium iodide, by Beyotime (2 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Fitc conjugated annexin 5, by Merck Group (1 mentions) Caspase 3 activity assay kit, by Beyotime (1 mentions) Annexin 5 phycoerythrin pe apoptosis detection kit, by Beyotime (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Thermo Fisher Scientific (1 mentions) Sybr green pcr master mix, by Thermo Fisher Scientific (1 mentions) Mirvana qrt pcr mirna detection kit, by Thermo Fisher Scientific (1 mentions) Rna pure rapid extraction kit, by BioTeke (1 mentions) Superscript 4 first strand synthesis system, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

FACScan ﬂow cytometer FACScan laser FACScan flow cytometry FACScan analyzer FACScan laser flow EPICS XL-MCL FACScan FACScan flow cytometry system FACScan flow cytometer EPICS XL-MCL FACScan flow cytometer

143 protocols using facscan

Quantifying Antibody Binding on Microspheres

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The average number of α-ICAM-1 mAbs on the surfaces of the imaging probes was determined using flow cytometry. Non-fluorescent microspheres (Polysciences, Inc., Warrington, PA) conjugated to α-ICAM or IgG were incubated with either FITC conjugated goat-α-mouse Ab or its isotype control. Microspheres were centrifuged, washed twice, and resuspended in PBS. The fluorescence intensities of the microspheres were measured using a FACScan (Coulter EPICS XL) equipped with the ‘System Work II’ software.
To quantify the number of copies of the molecules conjugated on the surface of the microspheres, mean fluorescence Intensity (MFI) was plotted against a calibration curve constructed as previously described.[11 (link)]

Frimmel S., Zandi S., Sun D., Zhang Z., Schering A., Melhorn M.I., Nakao S, & Hafezi-Moghadam A. (2017). Molecular Imaging of Retinal Endothelial Injury in Diabetic Animals. Journal of Ophthalmic & Vision Research, 12(2), 175-182.

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Cell Death Apoptosis Analysis by Flow Cytometry

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Cell death (apoptosis) was studied by flow cytometry using a FACScan
(fluorescence-activated cell sorter) flow cytometer (Coulter Corporation,
Hialeah, FL). For this assay, 24-well plates were used, seeding at
a cell density of 5 × 10⁴ for B16-F10, 6 × 10⁴ for HT-29, and 15 × 10⁴ for HepG2. The cells
were incubated for 24 h with 1.5 mL of culture medium. Subsequently,
the cells were treated with compounds 1 and 2 in triplicate for 4, 24, and 72 h, at the concentration of their
corresponding IC₅₀. The cells were collected and resuspended
in a binding buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 2.5 mM
CaCl₂). Annexin V-FITC conjugate (1 μg/mL) was then
added and incubated for 1 h at room temperature in darkness. Just
before analysis by flow cytometry, cells were stained with 5 μL
of 1 mg/mL PI solution. In each experiment, approximately 10 ×
10³ cells were analyzed, and the experiment was duplicated
twice.

Medina-O’Donnell M., Vega-Granados K., Martinez A., Sepúlveda M.R., Molina-Bolívar J.A., Álvarez de Cienfuegos L., Parra A., Reyes-Zurita F.J, & Rivas F. (2022). Synthesis, Optical Properties, and Antiproliferative Evaluation of NBD-Triterpene Fluorescent Probes. Journal of Natural Products, 86(1), 166-175.

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Cell Cycle Analysis via FACS

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Sub-G1population staining was performed as previously reported [73 (link)] and the analysis was performed by FACScan (Coulter).

Hernandez-Muñoz I., Figuerola E., Sanchez-Molina S., Rodriguez E., Fernández-Mariño A.I., Pardo-Pastor C., Bahamonde M.I., Fernández-Fernández J.M., García-Domínguez D.J., Hontecillas-Prieto L., Lavarino C., Carcaboso A.M., de Torres C., Tirado O.M., de Alava E, & Mora J. (2016). RING1B contributes to Ewing sarcoma development by repressing the NaV1.6 sodium channel and the NF-κB pathway, independently of the fusion oncoprotein. Oncotarget, 7(29), 46283-46300.

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Quantification of Apoptosis by Flow Cytometry

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Apoptosis was quantified by flow cytometry, using a FACScan (fluorescence-activated cell sorter) flow-cytometer (Coulter Corporation, Hialeah, FL, USA), analyzing between 5000 and 10,000 events. HepG2 cells were plated at 1.5 × 10⁵ cells per well on a 24-well plate, in 2 mL of medium, and treated with OADP for 24, 48, and 72 h, at the IC₂₀, IC₅₀, and IC₈₀ values. Cells were collected and resuspended in binding buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 2.5 mM CaCl₂). Annexin V-fluorescein isothiocyanate (FITC) conjugate (1 µg/mL) was then added and incubated for 30 min at rt in the dark. Just before the FACS analysis, we stained the cells with 20 µL of 1 mg/mL propidium iodide (PI) solution. All experiments were performed two times, in triplicate, including the control.

Jannus F., Medina-O’Donnell M., Rivas F., Díaz-Ruiz L., Rufino-Palomares E.E., Lupiáñez J.A., Parra A, & Reyes-Zurita F.J. (2020). A Diamine-PEGylated Oleanolic Acid Derivative Induced Efficient Apoptosis through a Death Receptor and Mitochondrial Apoptotic Pathway in HepG2 Human Hepatoma Cells. Biomolecules, 10(10), 1375.

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Cell Cycle and Apoptosis Analysis

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Flow cytometry was utilized to detect the cell cycle and apoptosis. Cells were seeded in 6-well plates and cultured for 48 h and then were collected with trypsin digestion solution, washed twice PBS. For cell cycle analysis, cells were resuspended in 200 μl PBS with 10 μl propidium iodide (PI) and incubated at room temperature in the dark for 15 min. For cell apoptosis analysis, cells (1 × 10⁵ cells/well) were seeded in 6 well-plate, then washed with cold PBS and centrifuged at 2000 rpn for 10 min to resuspend in binding buffer. Then 5 μl Annexin V-FITC was added and incubated for 15 min in the dark. 5 μl propidium iodide (PI) was added to the cells before analyzed. Flow cytometry analysis was carried out using a FACS can (Beckman Coulter, Fullerton, CA, USA).

Ding S., Zhang H., Zhao X., Dang J, & Li G. (2020). UNC5A, an epigenetically silenced gene, functions as a tumor suppressor in non-small cell lung cancer. Saudi Journal of Biological Sciences, 27(11), 3009-3017.

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Apoptosis Evaluation by Flow Cytometry

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Cell apoptosis analysis was performed using propidium iodide (PI) and fluorescein isothiocynate (FITC)-conjugated annexin V stain and followed by flow cytometry. In brief, cells after corresponding administration were washed twice by cold phosphate-buffered saline (PBS; Sigma-Aldrich) and then stained with PI/FITC–annexin V in the presence of 50 μg/ml RNase A (Sigma-Aldrich). Afterward, cells were incubated for 1 h at room temperature in the dark. Flow cytometry analysis was performed by FACScan (Beckman Coulter, Brea, CA, USA) to differentiate apoptotic cells (annexin V⁺ and PI⁻) from necrotic cells (annexin V⁺ and PI⁺). Data were analyzed using the FlowJo software.

Lu P., Gu Y., Li L., Wang F., Yang X, & Yang Y. (2018). Long Noncoding RNA CAMTA1 Promotes Proliferation and Mobility of the Human Breast Cancer Cell Line MDA-MB-231 via Targeting miR-20b. Oncology Research, 26(4), 625-635.

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Detecting Cell Apoptosis by FITC Annexin V

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Cell apoptosis was detected by FITC Annexin V staining. First, the cells were harvested and washed with ice‐cold phosphate‐buffered saline (PBS), and then subjected to the FITC Annexin V apoptosis detection kit (BD Pharmingen) for staining. Finally, the cells were analyzed using a FACScan instrument (Beckman Coulter) and the data were measured using WinMDI 2.9.

Li Y., Zu L., Wu H., Zhang F., Fan Y., Pan H., Du X., Guo F, & Zhou Q. (2021). MiR‐192/NKRF axis confers lung cancer cell chemoresistance to cisplatin via the NF‐κB pathway. Thoracic Cancer, 13(3), 430-441.

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Annexin V-FITC and PI Apoptosis Assay

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Cell apoptosis analysis was performed using propidium iodide (PI)/fluorescein isothiocynate (FITC)-conjugated annexin V staining. Briefly, treated cells were washed with phosphate-buffered saline (PBS) and fixed in 70% ethanol. The cells were then washed twice with PBS and stained with PI/FITC–annexin V in the presence of 50 μg/ml RNase A (Sigma-Aldrich, St. Louis, MO, USA). Finally, they were incubated at room temperature for 1 h in the dark. Flow cytometry analysis was performed using a FACScan (Beckman Coulter, Fullerton, CA, USA).

Wang P., Cai Y., Lin D, & Jiang Y. (2017). Gamma Irradiation Upregulates B-cell Translocation Gene 2 to Attenuate Cell Proliferation of Lung Cancer Cells Through the JNK and NF-κB Pathways. Oncology Research, 25(7), 1199-1205.

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Cell Viability and Proliferation Assays

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Cell viability was measured using Cell Counting Kit-8 (Dojindo, Japan). Cell cycle were analyzed by flow cytometry on a FACScan (Beckman Instruments, Fullerton, CA, USA). The cell-proliferation assays, colony-formation assays and cycle analysis were performed as described previously.^{42 (link)} Experiments were independently repeated in triplicate.

Wang Z.Q., Cai Q., Hu L., He C.Y., Li J.F., Quan Z.W., Liu B.Y., Li C, & Zhu Z.G. (2017). Long noncoding RNA UCA1 induced by SP1 promotes cell proliferation via recruiting EZH2 and activating AKT pathway in gastric cancer. Cell Death & Disease, 8(6), e2839-.

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Apoptosis Quantification via Flow Cytometry

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Flow cytometry was carried out with PI and FITC-conjugated annexin V staining (C1062M, Beyotime, China). PBS washed the fixed cells twice and PI/FITC-Annexin V stained, followed by hatch in the dark at 1 h. This assay was implemented by FACS can (Beckman Coulter, Fullerton, CA, USA). The data was analyzed by FlowJo software (Tree Star Software, San Carlos, California, US).

Dong D., Lun Y., Sun B., Sun H., Wang Q., Yuan G, & Quan J. (2020). Silencing of long non-coding RNA PCAT6 restrains gastric cancer cell proliferation and epithelial-mesenchymal transition by targeting microRNA-15a. General physiology and biophysics, 39(1).

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