Lactate content was measured using the Lactate-Glo Assay system (Promega). Briefly, 10 3rd instar larvae were homogenized in 150 μL of mammalian cell lysis solution on ice and centrifuged at 14,000 × g at 4 °C for 5 min to remove insoluble material. The supernatant was diluted 1:10 in HBSS (Thermo Fischer Scientific) and quantified according to the manufacturer’s protocol. Lactate content was normalized by protein content assessed by Bradford method.
Lactate glo assay system
Manufactured by Promega
Sourced in United States
The Lactate-Glo Assay system is a bioluminescent assay designed to detect and quantify lactate levels in biological samples. The assay utilizes a luciferase-based detection method to generate a luminescent signal proportional to the amount of lactate present in the sample.
Automatically generated - may contain errors
2 protocols using lactate glo assay system
1
Larval Lactate Quantification
2
Lactate Quantification in Perfusate
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Perfusate samples were collected at different time points (0, 30, 90, 150, 210, and 270 minutes) after perfusion start to quantify lactate levels in the perfusate using the Lactate-Glo Assay System (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol. For analysis, perfusate samples were diluted 1:50 with DPBS (Lonza), and relative luminescence units (RLU) were calculated using Lumat LB 9507 (Berthold Technologies, Zug, Switzerland) luminometer. L-lactate concentrations were measured by extrapolation using a standard curve.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!