For transport of the isolates from Lagos to Munich (Germany), strains were cultured on GC agar serum plates (see above), passaged twice and transferred into Portagerm Pylori (bioMérieux SA Mercy L’Etoile France) medium. In this state they were shipped to Munich (via DHL couriers).
Portagerm pylori
Manufactured by bioMérieux
Sourced in France
Portagerm pylori is a lab equipment product manufactured by bioMérieux. It is designed to facilitate the detection and identification of Helicobacter pylori, a bacterium commonly associated with gastritis and peptic ulcers.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by bioMérieux (1 mentions)
Tetracycline, by bioMérieux (1 mentions)
Clarithromycin, by bioMérieux (1 mentions)
Pylori agar, by bioMérieux (1 mentions)
Etest, by bioMérieux (1 mentions)
Cool transport container, by Sarstedt (1 mentions)
Generbag, by bioMérieux (1 mentions)
Generbox microaer, by bioMérieux (1 mentions)
7 protocols using portagerm pylori
1
Shipping Helicobacter pylori Isolates
2
Helicobacter pylori antibiotic resistance
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The biopsy specimens for histology were fixed in formalin, embedded in paraffin, sectioned, and stained with haematoxylin-eosin. The biopsy specimens for the bacterial culture were immediately placed in an appropriate transport medium (Portagerm-Pylori, bioMérieux, France) and then homogenised and cultured on both selective (Pylori agar, bioMérieux) and nonselective (10% horse blood agar, Kima, Italy) media. After seven days of incubation at 37°C under microaerophilic conditions, typical oxidase and catalase positive colonies were identified by API Campy strips (bioMérieux) and subsequently tested for antibiotic sensitivity (E test) [14 (link)]. The following antibiotics were tested: ampicillin, tetracycline, metronidazole, and clarithromycin (AB Biodisk, Sweden). The minimum inhibitory concentration (MIC) interpretative values used to define resistance (R) to each antibiotic are reported in Table 3 .
Histologically all patients had chronic nonatrophic gastritis.
Histologically all patients had chronic nonatrophic gastritis.
3
Isolation and Antibiotic Susceptibility of H. pylori
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The biopsy samples obtained for bacterial culture were transported in a special H. pylori transport medium (i.e., Portagerm pylori from BioMérieux SA, Marcy l’Etoile, France), and inoculated after a few hours onto selective medium Pylori Agar (BioMérieux Italia). For 72 h, the plates were incubated at 37 °C in a microaerobic environment. After incubation, Gram stain and oxidase, catalase, and urease tests were used to identify the H. pylori colonies. To perform an E-Test on pylori agar, suspensions from the primary plates were prepared in a sterile solution. An E-Test strip (E-Test; AB Bio disk, Solna, Sweden) was placed onto each separate plate and immediately incubated in a microaerobic atmosphere at 37 °C for another 72 h. This procedure involved streaking an agar plate in three directions with a swab dipped into each bacterial suspension to create a lawn of growth. Isolated strains were screened for several antibiotic resistance (e.g., amoxicillin, clarithromycin, metronidazole, and levofloxacin) according to the recommendations of the European Committee on Antimicrobial Susceptibility Testing.
4
Transporting Bacterial Isolates Safely
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For transport of the isolates from Nigeria or South Africa to Munich (Germany), strains were cultured on GC agar serum plates, passaged at least twice and transferred into Portagerm Pylori (bioMérieux SA Mercy L’Etoile France) medium for shipment at room temperature, as described previously22 .
5
Gastric Antrum Sampling Protocol
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Samples of antrum were entered into a transport medium (Portagerm pylori, bioMérieux, Marc l'Etoile, France) for culturing and sent by courier in a cool transport container (Sarstedt, Orsay, France) to a referral laboratory. They were processed based on a previously described protocol (3, 4) .
6
H. pylori Antibiotic Susceptibility in Poland
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Evaluation of drug susceptibility was performed on the total of 108 strains of H. pylori originating from adult patients from West Poland. All the strains were isolated from gastric mucosa before treatment. Group 1 involved 66 strains isolated in years of 1998/1999. Group 2 comprised 42 isolates obtained in years of 2013/2014. Biopsies isolated from the prepyloric portion were immediately placed in a transport medium (Portagerm pylori; bioMerieux). The obtained biopsies were plated on Columbia agar supplemented with 7% of sheep blood and a set of antibiotics (H. pylori selective supplement Dent SR 147E; Oxoid). The incubation was performed in microaerophilic conditions (Generbag or Generbox microaer; bioMerieux) at the temperature of 37 °C for 4-7 days. For the drug susceptibility test a suspension of grown bacteria was used, in PBS, manifesting density 2 in McF scale. The cultured strains were identifi ed based on colony morphology, Gram staining and urease and catalase tests. All the research protocols were reviewed and approved by the Ethics Committee of the Poznan University of Medical Sciences, Poland.
7
H. pylori Infection Detection Protocol
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H. pylori infection was detected via cultivation of two biopsy samples taken from antrum of stomach in a transport medium (Portagerm pylori, bioMe´rieux, Marc l'Etoile, France) and Urease Breath Test (UBT) viaa CLOtest (Tri-Med Specialities, Osborne Park, Western Australia). In addition, two biopsy samples were taken from antrum and corpus of stomach for histopathologic assessment. Three different tests including UBT, histology and cultivation of H. pylori were performed for confirmation of infection and if the results of all tests were positive, the patient was considered as an infected individual for H. pylori.
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