Digital camera

Manufactured by Kodak

Sourced in Japan

The Digital Camera is a laboratory equipment designed to capture digital images. It is capable of converting visual scenes into electronic data that can be stored, processed, and transmitted digitally.

Automatically generated - may contain errors

Lab products found in correlation

Fluorescence microscope, by Kodak (3 mentions) C57bl 6 mice, by Jackson ImmunoResearch (2 mentions) 300 mesh carbon coated copper grid, by Electron Microscopy Sciences (2 mentions) Ecl kit, by GE Healthcare (1 mentions) Bcatm protein assay kit, by Thermo Fisher Scientific (1 mentions) Image analysis system, by Uvitec (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Insulin, by Cell Signaling Technology (1 mentions) Fabp4, by Cell Signaling Technology (1 mentions) Sqstm1 p62, by Cell Signaling Technology (1 mentions) Tomm20, by Abcam (1 mentions) Fluorescence microscope, by Zeiss (1 mentions) Nf κb p65, by Cell Signaling Technology (1 mentions) Phospho nf κb p65, by Cell Signaling Technology (1 mentions) F4 80, by BioLegend (1 mentions) Cellstar, by Greiner (1 mentions) Glutaraldehyde, by Electron Microscopy Sciences (1 mentions) Cm100, by Philips (1 mentions) Dcode universal mutation detection system, by Bio-Rad (1 mentions)

16 protocols using digital camera

Western Blot and Immunofluorescence Analysis

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Cells were lysed in buffer containing 50 mM HEPES, 150 mN NaCl (4.38 g), 1 mM EDTA, 1% (w/v) CHAPS and Sigma protease inhibitor cocktail. Subsequently the cell lysates were resolved by10% SDS-PAGE and transferred to nitrocellulose membrane. The following antibodies were used: GFP antibody (632381) was purchased from Cell Signaling Technology, Bcl-x antibody (610211) was purchased from Clontech. α-Tubulin antibodies (T5168) were purchased from Sigma-Aldrich. Bound antibodies were visualized with the ECL kit (GE Healthcare).
Two hundred ninety-three cells stably transfected with circular RNA expression vector upon were seeded onto poly-lysine coated glass coverslips in a 6-well plate, and induced with 1 μg/mL tetracycline. At 48 h after induction, the cells were fixed with 4% formaldehyde in 1× PBS for 20 min, washed three times with 1× PBS, and then permeabilized with 0.2% Triton X-100. The cover slips were then mounted with mounting medium (Vector shield's mounting medium with DAPI). Cells were visualized using an Olympus fluorescence microscope, and photographs were generated using a Kodak digital camera.

Wang Y, & Wang Z. (2015). Efficient backsplicing produces translatable circular mRNAs. RNA, 21(2), 172-179.

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Bronchoalveolar Lavage and Lung Imaging

Cited 1 time

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At killing, collection of BAL fluid was performed using 1 ml of sterile Hanks’ balanced saline buffer. The BAL protein concentration was determined by a BCATM Protein Assay kit (Thermo Scientific, Pittsburg, PA). BAL inflammatory cell counting was performed using a standard hemacytometer technique (Fu et al., 2009 (link)). As an additional parameter reflecting increased lung vascular leakiness, Evans blue accumulation in the lung tissue was evaluated as described elsewhere (Fu et al., 2009 (link)). At the end of the experiment, a thoracotomy was performed, and the lungs were perfused in situ via the left atrium with PBS containing 5 mM EDTA to flush the blood off the lungs. The left lung and right lung were excised and imaged by a Kodak digital camera.

Ke Y., Karki P., Zhang C., Li Y., Nguyen T., Birukov K.G, & Birukova A.A. (2019). Mechanosensitive Rap1 activation promotes barrier function of lung vascular endothelium under cyclic stretch. Molecular Biology of the Cell, 30(8), 959-974.

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Visualizing BlaCTXM-1 Gene Expression

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After the electrophoresis, the resulting gel was visualised using an image analysis system (UVITEC Cambridge, United Kingdom). The images were photographed with a digital camera (Kodak, Japan), as shown in Figure 1, to detect the BlaCTXM-1 gene.

Deku J.G., Duedu K.O., Ativi E., Kpene G.E, & Feglo P.K. (2021). Occurrence and distribution of extended-spectrum β-lactamase in clinical Escherichia coli isolates at Ho Teaching Hospital in Ghana. Ghana Medical Journal, 55(4), 298-307.

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Localization of TEAD4 and YAP by Immunofluorescence

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To determine the localization of TEAD4-FL, TEAD4-S and YAP, we performed immunofluorescence assay. In brief, cells were plated on coverslips to appropriate density. Transfected cells were fixed on the coverslips with 4% paraformaldehyde in 1 × PBS for 15 min at room temperature and washed with 1 × PBS three times. Cells were then permeabilized with 0.2% Triton X-100 for 10 min. After blocking in 3% bovine serum albumin for 30 min, slides were incubated with indicated antibodies (Flag or Myc, 1:100 dilution) antibody diluted in 1% bovine serum albumin for 2 h. Subsequently, slides were washed with 1 × PBS for three times, and then incubated with fluorophore-conjugated secondary antibodies for 1 h. The coverslips were then washed and mounted with mounting medium (Vector shield's mounting medium with 4,6-diamidino-2-phenylindole). Cells were visualized using an Olympus fluorescence microscope, and photographs were generated using a Kodak digital camera.

Qi Y., Yu J., Han W., Fan X., Qian H., Wei H., Tsai Y.H., Zhao J., Zhang W., Liu Q., Meng S., Wang Y, & Wang Z. (2016). A splicing isoform of TEAD4 attenuates the Hippo–YAP signalling to inhibit tumour proliferation. Nature Communications, 7, ncomms11840.

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Endothelial SOCS1 Knockout Mice in LPS-Induced Lung Injury

Cited 2 times

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All animal care and treatment procedures were approved by the Institutional Animal Care & Use Committee of University of Chicago and University of Maryland. C57/BL6 mice were obtained from Jackson Laboratories (Bar Harbor, ME). EC-specific SOCS1 knockout mice were generated by cross-breeding of the endothelial VECad-Cre-ER^T2 line (42 (link)) and SOCS1^flox/flox mice (43 (link)) in the C57B6 background, as we previously described (44 (link)). Both sets of mice were anesthetized with an intraperitoneal injection of ketamine (75 mg/kg) and xylazine (7.5 mg/kg). Then, sterile saline solution or LPS (0.75 mg/kg body wt.) were given intratracheally. For the evaluation of lung injury parameters, BAL fluid was collected after intratracheal injection of 1 ml of sterile Hanks balanced salt buffer and total cells and protein content in BAL was measured as described previously (45 (link)). The vascular leak was analyzed by injecting Evans blue dye (30 mg/kg) into the external jugular vein 2 h before the end of the experiment as described earlier (45 (link)) and following perfusion excised lungs were imaged by a Kodak digital camera.

Karki P., Cha B., Zhang C.O., Li Y., Ke Y., Promnares K., Kaibuchi K., Yoshimura A., Birukov K.G, & Birukova A.A. (2021). Microtubule-dependent mechanism of anti-inflammatory effect of SOCS1 in endothelial dysfunction and lung injury. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(4), e21388.

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Ultrastructural Analysis of Extracellular Vesicles

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Electron microscopy analysis was performed as already described [10 (link)]. Briefly, for scanning electron microscopy (SEM) the cells were fixed in PBS (Sigma-Aldrich) 2% glutaraldehyde (Sigma-Aldrich) and dehydrated with ethanol solutions. Next, samples were sputter-coated with an SCD040 Balzer Sputterer (Oerlikon Balzers, Balzers, Liechtenstein). Samples were analyzed with an SEM Philips 505 microscope (Philips, Amsterdam, Netherlands). Transmission electron microscopy was performed on concentrated EV preparations resuspended in PBS (Sigma-Aldrich) to analyze their ultrastructural morphology. According to proper dilutions, the samples were adsorbed to 300 mesh carbon-coated copper grids (Electron Microscopy Sciences) for 5 min in a humidified chamber at room temperature. EVs on grids were then fixed in 2% glutaraldehyde (Sigma-Aldrich) in PBS for 10 min and then briefly rinsed in milli-Q water. Grids with adhered EV were examined with a Philips CM 100 transmission electron microscope TEM at 80 kV, after negative staining with 2% phosphotungstic acid, brought to pH 7.0 with NaOH. Images were captured by a Kodak digital camera.

Barilani M., Peli V., Cherubini A., Dossena M., Dolo V, & Lazzari L. (2019). NG2 as an Identity and Quality Marker of Mesenchymal Stem Cell Extracellular Vesicles. Cells, 8(12), 1524.

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Protein Purification and Characterization

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Protein amounts in the extracts were determined by Bradford method, using bovine serum albumin as the standard. Proteins samples were analyzed by SDS-PAGE. The gels were captured with Kodak digital camera, and purity of the target protein was quantified with Image-Quant TL software. For Western blot analysis, the fusion protein was analyzed by SDS-PAGE and transferred to polyvinylidene fluoride membrane, immunoblotted, and treated with the anti-GFP antibodies diluted to 5000 folds and HRP conjugated secondary antibodies diluted to 5000 folds as well. The bands were appeared and photographed by adding 0.08% hydrogen peroxide and 4-chloro-1-naphthol solution (dissolved in 20% methanol). For observation the fluorescence emitted from the fusion protein on the gel, protein samples were mixed with SDS-PAGE sample buffer, incubated at 45 °C for 10 min, centrifuged briefly, and separated by SDS-PAGE. The gel was later visualized under UV light and photographed.

He X., Zhang S., Dang D., Lin T., Ge Y., Chen X, & Fan J. (2023). Detection of human annexin A1 as the novel N-terminal tag for separation and purification handle. Microbial Cell Factories, 22, 2.

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Histological and Immunofluorescence Analysis of Adipose Tissue

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Formalin-fixed paraffin-embedded tissues were cut into 5-µm-thick sections. Hematoxylin and eosin staining were conducted following standard protocols, and the stained tissue sections were visualized under a light microscope equipped a Kodak digital camera. Adipocyte size was determined using ImageJ software (NIH).
Immunofluorescence analysis was performed as previously described (Zhou et al., 2016 (link)) using primary antibodies against the following proteins and peptides: Trx2 (Santa Cruz; catalog no. sc-50336), FABP4 (Cell Signaling Technology; catalog no. 3544; and Abcam; catalog no. ab93945), p62/SQSTM1 (Cell Signaling Technology; catalog no. 7695), phospho–NF-κB p65 (Cell Signaling Technology; catalog no. 3033), NF-κB p65 (Cell Signaling Technology; catalog no. 8242), F4/80 (BioLegend; catalog no. 123116), TOMM20 (Abcam; catalog no. ab56783), insulin (Cell Signaling Technology; catalog no. 8138), and glucagon (Abcam; catalog no. ab10988). TUNEL (Roche; catalog no. 11684795910) staining performed according to the manufacturer’s instructions. Fluorescent images were acquired using a fluorescence microscope (Zeiss).

He F., Huang Y., Song Z., Zhou H.J., Zhang H., Perry R.J., Shulman G.I, & Min W. (2020). Mitophagy-mediated adipose inflammation contributes to type 2 diabetes with hepatic insulin resistance. The Journal of Experimental Medicine, 218(3), e20201416.

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Immunofluorescence Staining of Transfected Cells

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Transfected cells with appropriate density were fixed on the coverslips with 4% paraformaldehyde in 1× PBS for 15 min at room temperature and washed with 1× PBS three times. Cells were then permeabilized with 0.2% Triton X-100 for 10 min and washed with 1 × PBS three times. After blocking in 3% BSA for 30 min, slides were incubated with indicated antibodies (p-H2AX, SRSF1, or HA, 1:100 dilution) antibody diluted in 3% BSA for 2 h. Subsequently, slides were rinsed three times with 1× PBS for 5 min each and then incubated with fluorophore-conjugated secondary antibodies for 1 h. The cover slips were then washed with 1× PBS for three times and mounted with mounting medium (Vector shield's mounting medium with DAPI). Cells were visualized using an Olympus fluorescence microscope, and photographs were captured using a Kodak digital camera.

Sheng J., Zhao Q., Zhao J., Zhang W., Sun Y., Qin P., Lv Y., Bai L., Yang Q., Chen L., Qi Y., Zhang G., Zhang L., Gu C., Deng X., Liu H., Meng S., Gu H., Liu Q., Coulson J.M., Li X., Sun B, & Wang Y. (2018). SRSF1 modulates PTPMT1 alternative splicing to regulate lung cancer cell radioresistance. EBioMedicine, 38, 113-126.

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Cell Proliferation and Clonogenic Assays

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The cell proliferation/viability assay (48 h exposure) was performed as described, using the sulforhodamine B method [22 (link)]. The data are presented as ‘% Inhibition’ of the media control.
For the clonogenic assay, exponentially grown cells were seeded at the density of 1000 cells/well into 6-well plates (Cellstar^®, Greiner Bio-One GmbH, Kremsmünster, Austria) and incubated at 37 °C for 4 h to allow the cells to adhere. Compound addition was similar as above. After 24 h, the compound-containing conditioned media were replaced with fresh medium containing FBS (10%) and antibiotics. The incubation continued for another 10 days with a change of fresh medium every 5 days, the cells were fixed with methanol and stained with crystal violet (1 mg/mL in 20% ethanol), and the images were acquired with a Kodak digital camera.

Zhou Y.D., Li J., Du L., Mahdi F., Le T.P., Chen W.L., Swanson S.M., Watabe K, & Nagle D.G. (2018). Biochemical and Anti-Triple Negative Metastatic Breast Tumor Cell Properties of Psammaplins. Marine Drugs, 16(11), 442.

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