Histamine dihydrochloride, chloroquine diphosphate salt, VUF 8430 dihydrobromide, histamine trifluoromethyl toluidide (HTMT), osthole, capsaicin, and AMG9810 were obtained from the Sigma-Aldrich Corp. (St. Louis, MO, USA). With the exception of histamine and chloroquine, which were dissolved in water, all the other drugs were dissolved in DMSO. When the drugs were used in the behavior experiments, the drugs were diluted in saline, then the calcium imaging and the electrophysiological experiments, all the drugs were diluted in normal perfusion solution, the final concentration of DMSO or water did not exceed 0.5%.
Osthole
Manufactured by Merck Group
Sourced in United States
Osthole is a natural compound extracted from various plants. It is a colorless crystalline solid used as a research tool in laboratory settings. Osthole serves as a chemical standard and reference material for analytical and biochemical studies.
Automatically generated - may contain errors
Lab products found in correlation
Capsaicin, by Merck Group (2 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Chloroquine diphosphate salt, by Merck Group (1 mentions)
Vuf 8430 dihydrobromide, by Merck Group (1 mentions)
Amg9810, by Merck Group (1 mentions)
Histamine trifluoromethyl toluidide, by Merck Group (1 mentions)
Histamine dihydrochloride, by Merck Group (1 mentions)
Rhodamine 123, by Merck Group (1 mentions)
Cell counting kit 8 cck 8, by Merck Group (1 mentions)
Hoechst 33342, by Merck Group (1 mentions)
Annexin 5 propidium iodide apoptosis kit, by Thermo Fisher Scientific (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Eif2α, by Cell Signaling Technology (1 mentions)
P bcl 2, by Cell Signaling Technology (1 mentions)
α tubulin tuba, by Santa Cruz Biotechnology (1 mentions)
Cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
Bcl xl, by Cell Signaling Technology (1 mentions)
P70s6k, by Cell Signaling Technology (1 mentions)
Cleaved caspase 9, by Cell Signaling Technology (1 mentions)
13 protocols using osthole
1
Pharmacological Characterization of Neuroactive Compounds
2
Osthole Cytotoxicity and Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Osthole was purchased from Sigma-Aldrich; Merck KGaA (Darmstadt, Germany) (Fig. 1A ), dissolved in dimethyl sulfoxide (DMSO), and stored at −20°C. The final DMSO concentration used was <0.1%. The cell counting kit-8 (CCK-8), Hoechst 33342, and rhodamine 123 were purchased from Sigma-Aldrich; Merck KGaA. The Annexin V/propidium iodide (PI) apoptosis kit was purchased from Invitrogen; Thermo Fisher Scientific, Inc. (Waltham, MA, USA). All antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). All cell culture supplies were obtained from Gibco; Thermo Fisher Scientific, Inc.
3
Osthole Signaling Pathway Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Osthole (catalog number: O9265) was purchased from Sigma (St. Louis, MO, USA). Osthole was dissolved in DMSO to prepare a chemical stock for treatment. Antibodies against phosphorylated Akt (Ser473, catalog number: 4060), P70S6K (Thr421/Ser424, catalog number: 9204), S6 (Ser235/Ser236, catalog number: 2211), ERK1/2 (Thr202/Tyr204, catalog number: 9101), p90RSK (Thr573, catalog number: 9346), JNK (Thr183/Tyr185, catalog number: 4668), total Akt (catalog number: 9272), P70S6K (catalog number: 9202), S6 (catalog number: 2217), ERK1/2 (catalog number: 4695), p90RSK (catalog number: 9335), JNK (catalog number: 9252), IRE1α (catalog number: 3294), eIF2α (catalog number: 5324), Bak (catalog number: 12105S), and Bax (catalog number: 2772) were purchased from Cell Signaling Technology (Beverly, MA, USA). Bcl-xL, p-Bcl-2, cleaved caspase 3 and cleaved caspase 9 were also purchased from cell Signaling Technology. Antibodies against GRP78 (catalog number: sc-13968), ATF6 α (catalog number: sc-166659), and α-tubulin (TUBA, catalog number: sc-32293) were purchased from Santa Cruz Biotechnology, Inc (Santa Cruz, CA, USA). Inhibitors of ERK1/2 (U0126, catalog number: E1282) and JNK (SP600125, catalog number: E1305) were purchased from Enzo Life Sciences, Inc (Farmingdale, NY, USA), and a PI3K/Akt inhibitor (LY294002, catalog number: 9901) was purchased from Cell Signaling Technology, Inc.
4
Vascular Smooth Muscle Contractility Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BaCl2, glucose, magnesium sulfate (MgSO4), potassium phosphate monobasic (KH2PO4), KCl, sodium chloride (NaCl), sodium hydrogen carbonate (NaHCO3), CaCl2, and urethane were sourced from Daejung Chemicals & Metals Co., Ltd. (Siheung, Republic of Korea). Dimethyl sulfoxide (DMSO) was purchased from Junsei (Tokyo, Japan). Phenylephrine, acetylcholine (Ach), and ethylene glycol-bis(2-aminoethylether)-N,N‚N′,N′-tetraacetic acid (EGTA), Ang II, SK&F96365, nifedipine, imperatorin (>98.0% purity verified by HPLC, CAS: 482-44-0), and osthole (≥95.0% purity verified by HPLC, CAS: 484-12-8) were purchased from Sigma Aldrich, Inc. (St. Louis, MO, USA). 4-AP, Glib, and TEA were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
5
Natural Compounds Screening Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Eugenol, β-escin, curcumin, berberine hydrochloride, sclareol, capsaicin, parthenolide, ellagic acid, glutathione, L -ascorbic acid were from Sigma–Aldrich (United Kingdom); osthole and pterostilbene were from Stratech (United Kingdom) and mitoquinol from Cayman Chemical Company (United Kingdom); all other NPs were components of the Puretitre natural compound library from Caithness Biotechnologies (United Kingdom). All of the above except Eugenol (70% ethanol), glutathione, and L -ascorbic acid (dH2O) were dissolved in dimethyl sulfoxide (DMSO, Sigma United Kingdom) and added to growth media from the following stock solutions prepared in those solvents: Eugenol, 500 mM; glutathione, 375 mM, L -ascorbic acid, 500 mM; osthole, 200 mM; pterostilbene, 200 mM; β-escin, 50 mM; curcumin, 50 mM; berberine hydrochloride, 200 mM; sclareol, 130 mM; capsaicin, 200 mM; parthenolide, 130 mM; ellagic acid, 33.3 mM, mitoquinol, 2.94 mM.
6
Osthole Enhances Bone Fracture Healing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Osthole (UHPLC 98 %) used in animal administration was purchased from LKT Laboratories, Inc. (MN, USA). Osthole reagent was freshly prepared by dissolution with 0.5 % (v/v) Tween 80 (Sigma-Aldrich, MO, USA) in distilled water. Three dosages of Osthole, 5 (low), 20 (middle) and 50 (high) mg/kg of body weight (derived from Li et al. [18 (link)]) were given to mice by daily oral gavage from week 1 (day 7) post-osteotomy until euthanasia, and mice in control group were fed with vehicle solvent only. Body weights were monitored throughout whole experimental process.
Animals in control and treatment groups were subject to X-ray radiography, micro-computed tomography (µ-CT), histology and molecular imaging assessments. Fractured and contralateral femurs were harvested at the end of experiment at week 2, 3 and 4 post-operation. Soft tissues were removed from the operated and contralateral femurs. The specimens were fixed within 4 % paraformaldehyde (Life Technologies, NY, USA) solution for 24 h. Internal fixation needle was removed from the intramedullary cavity and the femur specimens were stored in 70 % ethanol for µ-CT and histologic processing.
Animals in control and treatment groups were subject to X-ray radiography, micro-computed tomography (µ-CT), histology and molecular imaging assessments. Fractured and contralateral femurs were harvested at the end of experiment at week 2, 3 and 4 post-operation. Soft tissues were removed from the operated and contralateral femurs. The specimens were fixed within 4 % paraformaldehyde (Life Technologies, NY, USA) solution for 24 h. Internal fixation needle was removed from the intramedullary cavity and the femur specimens were stored in 70 % ethanol for µ-CT and histologic processing.
7
Coumarin Compound Protocols for Research
8
Cisplatin and Osthole Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Transfected cells were seeded into 96-well culture plates at a density of 5,000 cells/well overnight. Cells were then treated with osthole (10 μmol/L) (Sigma-Aldrich, St. Louis, MO, USA) and different concentrations of cisplatin (0~30 μmol/L) (Sigma-Aldrich) for 48 h. Subsequently, CCK-8 (10 μl) (Beyotime, Shanghai, China) was added to each well and incubated for 2 h at 37°C. The absorbance of the plates was measured at 450 nm by using a microplate reader (Bio-Tek Instruments, Inc., Norcross, GA, USA). Half maximal inhibitory concentration (IC50) of cisplatin was calculated according to the cell viability curve. Combination index (CI) with cisplatin and osthole was calculated via the following formula: CI=E(A+B)/(EA+EB-EA×EB). EA represents as the inhibitory rate caused by cisplatin; EB represents as the inhibitory rate caused by osthole; E(A+B) represents as the inhibitory rate caused by Combination treatment with cisplatin and osthole. It is considered as simple addition of the two drugs when CI ranges from 0.85 to 1.15; It is considered as simple addition of the two drugs when CI ranges from 0.85 to 1.15; It is considered as the synergistic effect of the two drugs when CI is greater than 1.15; It is considered as the antagonistic effect of the two drugs when CI is less than 0.85.
9
HPLC Analysis of Coumarins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HPLC analysis was carried out as previously described [27 (link)] on a liquid chromatograph (LaChrom Elite, Hitachi, Tokyo, Japan) using DAD detection (200–400 nm). Quantification was carried out at two wavelengths (λ): 254 and 330 nm.
Reference substances were obtained from bergapten, imperatorin, xanthotoxin, and psoralen from Roth (Karlsruhe, Germany); 5,7-dimethoxycoumarin, 4-hydroxy-6-methylcoumarin, 6-methylcoumarin, osthole, and umbelliferone from Sigma-Aldrich (St Louis, MO, USA); coumarin and scopoletin from Fluka (Bucha, Switzerland); 4-methylumbelliferone, 4,6-dimethoxy-2H-1-benzopyran-2-one, and skimmianine from ChromaDex (Irvine, CA, USA); and isopimpinellin, isoimperatorin, daphnetin 7-methyl ether, rutaretin, daphnetin, osthenol, bergaptol, daphnetin dimethyl ether, γ-fagarine, and 7-isopentenyloxy-γ-fagarine from ChemFaces (Wuhan, China).
Reference substances were obtained from bergapten, imperatorin, xanthotoxin, and psoralen from Roth (Karlsruhe, Germany); 5,7-dimethoxycoumarin, 4-hydroxy-6-methylcoumarin, 6-methylcoumarin, osthole, and umbelliferone from Sigma-Aldrich (St Louis, MO, USA); coumarin and scopoletin from Fluka (Bucha, Switzerland); 4-methylumbelliferone, 4,6-dimethoxy-2H-1-benzopyran-2-one, and skimmianine from ChromaDex (Irvine, CA, USA); and isopimpinellin, isoimperatorin, daphnetin 7-methyl ether, rutaretin, daphnetin, osthenol, bergaptol, daphnetin dimethyl ether, γ-fagarine, and 7-isopentenyloxy-γ-fagarine from ChemFaces (Wuhan, China).
10
Preparation of Osthole, Histamine, and FXF Solutions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Osthole (PubChem CID: 10228), histamine (PubChem CID: 774), and FXF (PubChem CID: 63002) were obtained from Sigma Aldrich (St. Louis, MO, USA, cat. no. O9265, H7125, F9427, respectively). Osthole was dissolved in 96% ethanol (Chempur, Piekary Śląskie, Poland, cat. no. 653964200); FXF was dissolved in 8% dimethyl sulfoxide (DMSO; Sigma Aldrich, St. Louis, MO, USA, cat. no. D2650); and histamine was dissolved in the medium used for cell culture. The solutions were filtered through 0.22 µm pore filters and stored at −20 °C for later dilution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!