Anti human il 8 antibody

Anti-GAPDH (6c5), and anti-AR antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-MMP1 and Anti-MMP13 antibodies were purchased from Bioss (bs-4597R, bs-0575R, Woburn, MA). Anti-Human IL-8 antibody was purchased from Peprotech (500-p28, Rocky Hill, NJ). Recombinant Human IL-8 was purchased from R and D Systems (208-IL-010, Minneapolis, MN). MMP1 inhibitor was purchased from EMD Millipore (#444250, Gibbstown, NJ) and MMP13 inhibitor was purchased from Sigma (CL-82198, St Louis, MO). Polyvinylidene defluoride membrane (PVDF) was from Thermo Fisher Scientific (Rochester, NY). Anti-mouse/rabbit secondary antibody for Western blot was from Invitrogen (Grand Island, NY).

The following drugs and antibodies were used in our experiments: paclitaxel (PTX) (Sigma-Aldrich, St. Louis, MO, USA), erlotinib (Selleck Chemicals, Houston, TX, USA), anti-MUC1 antibody (Thermo Scientific, Hudson, NH, USA), anti-phospho-EGFR Tyr1068 antibody (Cell Signaling Technology, Danvers, MA, USA), and anti-EGFR antibody (Proteintech Group, Chicago, IL, USA), anti-Histone H3 (acetyl K27) (H3K27Ac) antibody (Abcam, Cambridge, MA, USA), IL-6 antibody (Novus, Littleton, CO, USA), horseradish peroxidase (HRP)-linked secondary antibody (Cell Signaling Technology, Beverly, MA, USA), anti-β-actin antibody (Merck Millipore, Billerica, MA, USA), FITC-conjugated mouse anti-human CD227 (MUC1) (BD Pharmingen, San Diego, CA, USA), PE-conjugated mouse anti-human CD133/1 (AC133) (Mitenyi Biotec, Bergisch Gladbach, Germany), IL-6 neutralizing antibody (Sino Biological Inc., Beijing, China), and anti-human IL-8 antibody (PeproTech, Rocky Hill, NJ, USA).