The blood samples were centrifuged at 3000 rpm (10 min, 4 °C) to collect plasma, and then levels of albumin (ALB), total protein (TP), lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), blood urea nitrogen (BUN), and creatinine (CRE) were determined by Roche Automatic biochemical analyzer (cobas c311) according to the respective kits purchased from Roche Diagnostics GmbH (Shanghai, China).
Cobas c311 automatic biochemical analyzer
Manufactured by Roche
Sourced in China, Switzerland
The Cobas c311 is an automatic biochemical analyzer designed for clinical laboratory use. The core function of the Cobas c311 is to perform quantitative analysis of various biochemical parameters in biological samples, such as blood, serum, or urine.
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Lab products found in correlation
9 protocols using cobas c311 automatic biochemical analyzer
1
Biochemical Marker Analysis of Plasma
2
Biochemical Marker Profiling in Clinical Samples
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The plasma levels of albumin (ALB), alkaline phosphatase (ALP), alanine aminotransferase (ALT), ammonia (AMM), aspartate aminotransferase (AST), glucose (GLU), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C), total cholesterol (TC), triglyceride (TG), total protein (TP), and urea nitrogen (UN) were determined using commercially available kits (F. Hoffmann-La Roche Ltd, Basel, Switzerland) with the Roche automatic biochemical analyzer (Cobas c311, F. Hoffmann-La Roche Ltd, Basel, Switzerland).
3
Comprehensive Surgical Outcomes and Quality of Life Evaluation
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Record the time taken for surgery, intraoperative bleeding volume, length of the incision, number of dissected lymph nodes, and total length of hospital stay, and calculate the respective percentages
Evaluate the emotional status of the patients with HAMA scores and HAMD scores (the scores are positively related to the severity of depression and anxiety).5 (link)
Evaluate the quality of life in patients with PSQI scores and SF-36 scores (the PSQI score is negatively related to sleep quality, while the SF-36 score is positively related to overall quality of life)5 (link)
Detect inflammatory cytokine indexes including PCT, CRP, and IL-6 with a Roche Cobas c311 automatic biochemical analyzer.
Perform a 12-month follow-up visit to record the number of patients who have had recurrence or metastasis and the number of patients who have died and calculate the respective percentages.
Record the incidence of postoperative fat liquefaction, pulmonary infection, hoarseness, anastomotic fistula and arrhythmia and calculate the respective percentages.
4
HBsAg Detection and BDCA-2/ILT7 Immunohistochemistry
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The hepatitis B surface antigen (HBsAg) enhanced chemiluminescence detection kit was purchased from Roche Diagnostics. RNA extraction kit (cat. no. AP-MN-MS-RNA-250) was from Axygen; Corning Inc., and reverse transcription (RT) kit was purchased from Takara Biotechnology Co., Ltd. (cat. no. RR037A). Mouse anti-human BDCA-2 (cat. no. 748001) and ILT7 monoclonal antibodies (cat. no. 562500) were purchased from BD Pharmigen; BD Biosciences. Goat anti-mouse biotin-labeled secondary antibody (cat. no. 31800), diaminobenzidine (DAB) chromogenic reagent and immunohistochemistry (IHC) streptavidin peroxidase conjugated method (SP) kit were purchased from Zymed (Thermo Fisher Scientific, Inc.). The Labsystem Version 1.3.1 microplate reader was purchased from Bio-Rad Laboratories, Inc. The ABI 7700 Fast Quantitative PCR Reactor was from ABI (Thermo Fisher Scientific, Inc.). The Cobas c311 automatic biochemical analyzer was purchased from Roche Diagnostics. The C6015-2 B-ultrasonic instrument was purchased from SonoSite, Inc.
5
Comprehensive Liver Biochemical Analysis
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Liver samples were accurately weighed and homogenized (diluted 1:10) in Phosphate buffered saline (PBS) (Solarbio, Beijing, China) with a tissue crusher. The homogenization was 900 × g centrifuge for 10 min at 4°C and retained the supernatant. The protein content of the homogenate was measured using Folin-phenol reagent (16 (link), 17 (link)). The contents of albumin (ALB), cholesterol (CHO), high-density lipoprotein cholesterol (HDLC), low-density lipoprotein cholesterol (LDLC), glucose (GLUC), creatine kinase (CK), iron (IRON), magnesium (MG), calcium (CA), phosphate (PHOS), alkaline phosphatase (ALP), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and creatinine (CREA) were detected by Roche Cobas C311 automatic biochemical analyzer (Shanghai, China). The kits used were Cobas C311 products.
According to the product instructions, amylase (AMS), glucose-6-phosphate dehydrogenase (G-6-PD), malic enzyme (ME), pyruvate kinase (PK), acyl CoA oxidase (ACO), lipase (LPS), fatty acid synthase (FAS), carnitine acyl carnitine translocation (CACT), acetyl CoA carboxylase (ACC), lipoprotein lipase (LPL), superoxide dismutase (SOD), Phospholipid hydroperoxide glutathione peroxidase (GSH-PX), catalase (CAT) and malondialdehyde (MDA) were detected. SeeTable S1
According to the product instructions, amylase (AMS), glucose-6-phosphate dehydrogenase (G-6-PD), malic enzyme (ME), pyruvate kinase (PK), acyl CoA oxidase (ACO), lipase (LPS), fatty acid synthase (FAS), carnitine acyl carnitine translocation (CACT), acetyl CoA carboxylase (ACC), lipoprotein lipase (LPL), superoxide dismutase (SOD), Phospholipid hydroperoxide glutathione peroxidase (GSH-PX), catalase (CAT) and malondialdehyde (MDA) were detected. See
6
Serum Biomarker Quantification Protocol
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The concentrations of serum IgG were determined using a goat IgG ELISA Quantitation kit (Bethyl Laboratories, TX, USA) according to the manufacturer’s protocol as described by Morales-de la Nuez et al. (47 (link)). The concentrations of serum glucose (48 (link)) and urea nitrogen (49 (link)) were analyzed using the respective commercial kits (Jiancheng Bioengineering Institute, Nanjing, China) according to the manufacturer’s protocols. Serum TP, Alb, Glob, total cholesterol, triglycerides, HDL-C, and LDL-C were measured using a Cobas c311 automatic biochemical analyzer (Roche, Basel, Switzerland).
7
Hormonal and Lipid Profile Analysis
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Serum testosterone, estradiol, and progesterone levels were measured using commercial iodine [125I] RIA Kits (Beijing North Biotechnology Research Institute). Serum HDL (High Density Lipoprotein), LDL (Low Density Lipoprotein), CHO (Cholesterol), and TG (Triglyceride) levels were measured using the cobas c311 automatic biochemical analyzer (Roche, Shenzhen SHAHE Hospital). The intra- and inter-assay errors among all assays were less than 10 and 15%, respectively.
8
Serum Biochemistry and Fecal Nitrogen Analysis
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At the end of each trial, blood samples were collected from the jugular veins using 10-ml centrifuge tubes and centrifuged at 3,000 × g and 4°C for 15 min to recover the serum. Then, the samples were stored at −20°C until biochemical analyses. The contents of total protein (TP), albumin, urea nitrogen (UN), and NH3-N in serum were measured using a Cobas C311 automatic biochemical analyzer (Roche, Basel, Switzerland) and respective commercial assay kits (Leadman, Beijing, China).
During the 5 days before each trial ended, nearly 100 g of fresh feces was collected per pen per day and then added into 10% H2SO4 solution (W/V, 1:10). The feces collected were well-mixed according to the pens, dried in a forced air oven at 60°C for 72 h, and then ground through a 1-mm screen. The fecal samples were stored at −20°C before further analyses (20 (link)). The fecal nitrogen content was determined using a Mobile Injection Analyzer (AA3, Seal, Norderstedt, Germany).
During the 5 days before each trial ended, nearly 100 g of fresh feces was collected per pen per day and then added into 10% H2SO4 solution (W/V, 1:10). The feces collected were well-mixed according to the pens, dried in a forced air oven at 60°C for 72 h, and then ground through a 1-mm screen. The fecal samples were stored at −20°C before further analyses (20 (link)). The fecal nitrogen content was determined using a Mobile Injection Analyzer (AA3, Seal, Norderstedt, Germany).
9
Plasma Biochemical Parameters Analysis
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The plasma biochemical parameters, including albumin (ALB), alkaline phosphatase (ALP), alanine aminotransferase (ALT), amylase (AMS), aspartate aminotransferase (AST), urea nitrogen (UN), cholinesterase (CHE), cholesterol (CHO), glucose (GLU), high-density lipoprotein-cholesterol (HDL-C), lactate dehydrogenase (LDH), low-density lipoprotein-cholesterol (LDL-C), ammonia (NH3), triglyceride (TG), and total protein (TP) were analyzed using commercially available biochemical kits (Leadman Biochemistry Technology Company, Beijing, China) on Cobas c311 Automatic Biochemical Analyzer (Cobas c311, F. Hoffman-La Roche Ltd., Basel, Switzerland), according to the manufacturers’ instructions.
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